Role of influenza virus-specific CD8+ killer T cell on the prevention of per-nasal infection

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 12671665
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Osaka University
• Principal Investigator: TAMURA Manabu Osaka University, School of Medicine Otorhinolaryngology, Associate Professor, 医学系研究科, 助教授 (50273644)
• Co-Investigator(Kenkyū-buntansha): KURANE Ichiro National Institute of Infections Diseases, Dept. of Virology, Chief, ウイルス部, 部長 (90278656)
• Project Period (FY): 2000 – 2001
• Keywords: mucosal immunity / influenza virus

Research Abstract

Upper air way infection is one of the most important disease in otorhinolaryngology field. Recently, the research of mucosal immunity has advanced in intestine and oral/nasal cavity. When we think about vaccination against viral or bacterial infection, oral immunization or nasal immunization would be the most attractive method. We have investigated the mucosal immunity by using mouse and influenza virus in order to develop the nasal or oral vaccine in future.
So far, we have developed several influenza virus specific T cell clones by immunizing the mouse with influenza virus via nasal infection. The epitopes, which each T cell clone recognized, were determined as the 9mer peptide. Mice were immunized with the 9mer peptide in the presence of adjuvant via subcutaneous route, and influenza virus specific T cell clones were developed.
The vector, which enable the gene transfer to the cells of intestine, has been developed by using chitosan. We are constructing the vector including the CTL epitope of influenza virus We are going to analyze the immunological response of the mouse which were immunized with the newly constructed vaccine.