2001 Fiscal Year Final Research Report Summary
Trial to reorganize retino-geniculate pathway in adult rats
| Project/Area Number |
12671706
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka University |
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Principal Investigator |
INOUE Tetsu Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (60263282)
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| Project Period (FY) |
2000 – 2001
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| Keywords | peripheral nerve graft / lateral geniculate body / retina / optic nerve / relay cell / bridge |
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| Research Abstract |
To reorganize a retino-geniculate pathway in adult mammals, I tried to make a bridge model between the retina and the dorsolateral geniculate body (LGd) with an autologous peripheral nerve graft. In female Wistar rats, 4 - 6 months of age, were anesthetized with pentobarbital (50 mg / kg). According to the way developed by Vidal-Sanz et al. (1997), a segment of the common peroneal nerve was grafted to the left optic nerve stump. To insert the free end of the graft into the contralateral LGd, I tackled the approach developed by Carter and Jhaveri (1997) ; the caudal half of the LGd was exposed through the cavity of an caudrolateral cerebral cortex. Then the graft end was inserted into the LGd with a glass electrode.
The grafted animals could not survive more than one year until the surface of the cortical cavity was made lass than 3 mm x 3 mm in area. In the seven grafted animals survived more than one year, two animals served histological examination. After perfusion-fixation with 4 % paraformaldehyde, coronal brain cryosections were made and stained with Eosin or Neutral Red. Under light microscope observation, the peroneal nerve graft was inserted into the dorsolateral LGd. Thus the animal model could be made as a basis for testing the functional reconnection of regenerating retinal axons to LGd relay cells.
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