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2001 Fiscal Year Final Research Report Summary

Genomic Study on Keloids and Hypertrophic Scars

Research Project

Project/Area Number 12671753
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionNIPPON MEDICAL SCHOOL

Principal Investigator

HYAKUSOKU Hiko  Nippon Medical School, Medical Science, Professor, 医学部, 教授 (00165135)

Co-Investigator(Kenkyū-buntansha) NAKAZAWA Nando  Nippon Medical School, Medical Science, Lecturer, 医学部, 講師 (20010051)
MURAKAMI Masahiro  Nippon Medical School, Medical Science, Instructor, 医学部, 助手 (00239500)
Project Period (FY) 2000 – 2001
KeywordsKELOID / HYPERTROPHIC SCAR / DNA-MICROARRAY / TUMOR SUPPRESSORS / SNP / PATHOLOGICAL GENOMICS / 細胞増殖 / Pathological Genomics
Research Abstract

We have studied and are continuing to study the cellular and molecular mechanism of pathogenesis of keloids and hypertrophic scars to develop the effective therapeutic procedure for the scar-forming diseases. The scope of this research is also directed further to elaborate clinical diagnostic and prognostic methods, and eventually to perform scar-free surgical operations.
The results are as follows ;
1) The tissue LDH levels assayed using a 1H-NMR technic were found to be closely associated with diagnostic and pathological gradings of the disease : a 1H-NMR assay of the tissue LDH levels provides effective conclusive diagnostic criteria for the disease.
2) Distribution of SNP of tumor suppressor gene, p53, at codon-72 region among keloid and hypertrophic scar patients was analyzed using RFLP method. It was indicated that Arginine-encoding sequence (CGC) and Proline-encoding sequence (CCC) confer genetic predisposition to earlobe keloids and hypertrophic scars, respectively.
3) To investiga … More te the function of P53 proteins with different amino - acid at codon-72 region, cells which express either Arg-P53 or Pro-P53 protein were grown in the various culture conditions. Arg-P53 cells were found to be more sensitive to high temperature conditions than Pro-P53 cells.
4) The pathological genomics of keloids was performed using the cDNA microarray technic after extracting mRNA from keloid fibroblastic cells in culture. Eight genes and 17 genes were expressed specificly from earlobe keloid cells and chest keroid cells, respectively. Thrombin receptor gene, KIAA0367 protein gene and matrilin-2 gene were typical genes expressed specifically in both of the keloid fibroblastic cells. Screening for the gene that is known to be involved in the growth regulating mechanism of cells revealed that a tumor suppressor gene is expressed only in the normal control skin fibroblasts but not in keloid cells. The result implies that the growth suppressor gene is implicated in the pathogenesis of keloid lesion. Less

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 王 春梅, 百束比古, 他: "1H-NMR法を用いた瘢痕組織の代謝学的分析-ケロイドと肥厚性瘢痕の分類-"Progress in Medicine. 20・11. 198-200 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 王 春梅, 百束比古, 他: "肥厚性瘢痕素因およびケロイド体質の分子伝学的解析"日形会誌. 21・4. 241-260 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 百束比古, 秋元正宇: "顔面熱傷瘢痕治療における遊離皮弁とその応用"形成外科. 43・8. 775-783 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石切 致, 王 春梅, 他: "1H-NMR法を用いたケロイド・肥厚性瘢痕の代謝学的研究"日形会誌. 21・8. 472-480 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 百束比古: "小川 龍(監修)坂本篤裕(編) 「内視鏡手術の術中管理とトラブル」"真興交易(株)医書出版部(分担執筆). 10 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chun-Mei Wang, Hiko Hyakusoku et al.: "1H-NMR Analysis of Metabolites of Scarring Tissues : Application for Diagnosis of Keloids and Hypertrophic Scars"Progress in Medicine. 20. 198-200 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chun-Mei Wang, Hiko Hyakusoku and Goro Asano: "Genetic Predisposition to Keloid and Hypertrophic Scar"J. Jpn. P.R.S.. 21. 241-246 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiko Hyakusoku, Masataka Akimoto et al.: "Application of Free Flap on Clinical Treatment of Facial Scars after Burn"Keisei Geka. 43. 775-783 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] "1H-NMR Analysis of Metabolites of Keloids and Hypertrophic Scars"J. Jpn. P.R.S.. 21. 472-480 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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