2001 Fiscal Year Final Research Report Summary
Study of skin reconstruction by using the technique of tissue engineering
| Project/Area Number |
12671754
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
KUMAGAI Norio Faculty of Medicine, Dept of Plastic Surgery, St. Marianna University School of Medicine, Professor, 医学部, 教授 (30103477)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Hajime Faculty of Medicine, Dept of Plastic Surgery, St. Marianna University School of Medicine, Assistant professor, 医学部, 講師 (60193603)
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| Project Period (FY) |
2000 – 2001
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| Keywords | fibrin / keratinocyte / culture / scaffold / tissue engineered epithelium / tissue engineered dermis / tissue engineered skin |
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| Research Abstract |
In this investigation, tissue engineered skin was designed by using the fibrin and other one. Keratinocytes were cultured onto the fibrin gel matrix but keratinocytes secreted protease digested fibrin gel easily. Therefore, the mechanical strength of fibrin gel matrix as the scaffold was labile. When one soluble connective tissue material (patent pending) was mixed in the fibrin gel matrix, the protease digestion by keratinocytes was protected. Furthermore, by using the new type fibrin scaffold, dermal fibroblasts could be cultured in gel matrix and then keratinocytes onto the gel matirix. From these results, this new type fibrin scaffold might be useful device such as tissue-engineered skin. Now we attempted to apply to this tissue-engineered skin by using new type scaffold clinically.
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