2002 Fiscal Year Final Research Report Summary
Mechanism of ischemia-reperfusion injury
| Project/Area Number |
12671756
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
UEDA Kouichi Osaka Medical College Faculty of Medicine Associate Professor, 医学部, 助教授 (90257858)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Ryotaro Osaka Medical College Faculty of Medicine Associate Professor, 医学部, 助教授 (10124760)
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| Project Period (FY) |
2000 – 2002
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| Keywords | ischemia-reperfusion / macropharge / Dichloromethylene diphosphonate / rat skin flaps |
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| Research Abstract |
The purpose of this study is to evaluate whether macropharge is related to ischemia-reperfusion injury. The concern of macropharge was investigated by immuno-histochemical study in the ischemia-reperfusion model of rat skin flaps. The femoral artery and vein were clamped above and below the epigastric vessels for 11 h and then the clamp was released. Histological examination 12 h after reperfusion showed macropharge invasion into the flap and leukocyte invasion was recognized 24 h after reperfusion. Peroxidase activity in the flap presented a peak 48 h after reperfusion.
Dichloromethylene diphosphonate (DMDP) was administered intravenously just before elevation of the right abdominal epigastric flap. However the effect by DMDP to ischemia-reperfusion injury was not recognized.
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