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2001 Fiscal Year Final Research Report Summary

Study on the role of heat shock protein 27 in the process of development and regeneration of dental pulp

Research Project

Project/Area Number 12671765
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionNiigata University

Principal Investigator

OHSHIMA Hayato  Graduate School of Medical and Dental Sciences, Niigata University, Professor, 大学院・医歯学部総合研究科, 教授 (70251824)

Co-Investigator(Kenkyū-buntansha) SATO Takuichi  Tohoku University, Graduate School of Dentistry, Assistant, 大学院・歯学研究科, 助手 (10303132)
KAWANO Yoshiro  Graduate School of Medical and Dental Sciences, Niigata University, Assistant, 大学院・医歯学部総合研究科, 助手 (60303129)
MAEDA Takeyasu  Graduate School of Medical and Dental Sciences, Niigata University, Professor, 大学院・医歯学部総合研究科, 教授 (40183941)
Project Period (FY) 2000 – 2001
KeywordsHeat shock protein (Hsp) 25 / 27 / Dental pulp / Odontoblasts / Tooth replantation / Cavity preparation / Tooth development / Regeneration / Rat
Research Abstract

The present study aims to clarify the functional significance of heat shock protein (Hsp) 25/27 during tooth development and pulpal regeneration. The present study demonstrated that Hsp 25/27 was expressed exclusively in fully-differentiated odontoblasts during tooth development. For the observation of pulp regeneration, Wistar rats, 4-week-old and 100-day-old, were used for tooth replantation and cavity preparation, respectively. Cavity preparation caused an edematous reaction between the injured odontoblasts and predentin, but the immunoreaction for Hsp 25/27 remained in the injured odontoblasts. Subsequently, Hsp 25/27-immunoreactivity disappeared in the degenerated odontoblast layer after 12 hours. On postoperative 3 days, newly differentiated odontoblasts replaced the degenerated odontoblasts, and became to exhibit the immunoreaction for Hsp 25/27. Tooth replantation also caused the disappearance of Hsp 25/27-immunopositive cells at the initial stages. On postoperative 5days, plump cells with clear nucleoli at the dentin-pulp border became to show Hsp 25/27-immureactivity. These findings indicate that newly differentiated odontoblasts acquire the immunoreaction for Hsp 25/27 in the regenerated pulpal tissue after both cavity preparation and tooth replantation. There was no Hsp 25/27-immunopositive cell along the pulp-dentin border in the case of bone-like tissue formation in the pulp space following tooth replatation. Thus, the alignment of Hsp 25/27-immunopostive cells along the pulp-dentin border is suggestive of the decisive factor to induce the reparative dentin formation after tooth replantation.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Ohshima, H: "Alteration in the expression of heat shock protein (Hsp) 25-immunoreactivity in the dental pulp of rat molars following tooth replantation"Archives of Histology and Cytology. 64. 425-437 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohshima, H: "Responses of odontoblasts to cavity preparation in rat molars as demonstrated by immunocytochemistry for heat shock protein (Hsp) 25"Archives of Histology and Cytology. 64. 493-501 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otsuka, Y: "Possible role of heat shock protein (Hsp) 25 in the enamel organ during amelogenesis in the rat molar"Archives of Histology and Cytology. 64. 369-378 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohshima, H: "Expression of heat shock protein (Hsp) 25-immunoreactivity in the dental pulp and enamel or an during odontogenesis in the rat molar"Connective Tissue Research. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamura, H: "Different distributions of immunocompetent cells in the dentogingival junction during root formation in rat molars"Journal of Periodontal Research. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohshima, H: "Functional significance of Msx2 gene during tooth development"Dentin Pulp Complex : Proceeding of the International Conference on Dentin Pulp Complex 2001. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大島勇人: "ザ・クインテッセンスYear Book今日の治療指針'01"クインテッセンス出版. 522 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohshima, H.: "Alteration in the expression of heat shock protein (Hsp) 25-immunoreactivity in the dental pulp of rat molars following tooth replantation"Archives of Histology and Cytology. 64 (4). 425-437 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohshima, H.: "Responses of odontoblasts to cavity preparation in rat molars as demonstrated by immunocytochemistry for heat shock protein (Hsp) 25"Archives of Histology and Cytology. 64 (5). 493-501 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otsuka, Y.: "Possible role of heat shock protein (Hsp) 25 in the enamel organ during amelogenesis in the rat molar"Archives of Histology and Cytology. 64 (4). 369-378 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohshima, H.: "Expression of heat shock protein (Hsp) 25-immunoreactivity in the dental pulp and enamel organ during odontogenesis in the rat molar"Connective Tissue Research. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamura, H.: "Different distributions of immunocompetent cells in the dentogingival junction during root formation in rat molars"Journal of Periodontal Research. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohshima, H.: "Functional significance of Msx2 gene during tooth development"Dentin Pulp Complex: Proceeding of the International Conference on Dentin Pulp Complex 2001. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohshima, H.: "Quintessence, Year Book, A Guide for Today's Treatment '01"Quintessence Publishing Co.. 522 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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