2002 Fiscal Year Final Research Report Summary
Effects of retinoic cid on adhesion molecules involving desmosome and hemidesmosome in gingival epithelial cells
| Project/Area Number |
12671778
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
HATAKEYAMA Setsuko Iwate Medical University, School of dentistry, Lecturer, 歯学部, 講師 (70048495)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Gingival epithelium / desmosome / hemidesmosome / retinoic acid / desmoglein / desmocollin / elecron microscopy / cytokeratin |
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| Research Abstract |
Although retinoic acid was reported to compromise desmosome expression in the human epidermis through downregulation of desmosomal cadherins, it is unclear whether retinoic acid inhibits the formation of desmosomes and/or hemidesmosomes in oral epithelia. Whether retinoic acid disintegrated cell-cell and cell-matrix adhesions of oral epithelia was investigated with stratified gingival epithelial (GE1) cells. Ultrastructurally, retinoic acid-treatment (1 μM, 5-10 days) drastically reduced the number of desmosomes and completely destroyed hemidesmosomes. Keratinocyte phenotypes disappeared, accompanied by loss of tonofilaments. The transcripts of desmoglein 1 (ANOVA, P<0.01), desmocollin 1 (P<0.05), and keratin 13 (P<0.05) were significantly decreased, but expression of α6 and β4 subunits of integrin, components of hemidesmosomes, were not changed. These results indicated that retinoic acid also disintegrated desmosomes by depriving the cells of desmoglein 1 and desmocollin 1 in the oral mucosal epithelium. The decrease of keratin filaments, the intracellular cytoskeletal attachments to hemidesmosomes, was probably a cause of hemidesmosome loss.
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