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2002 Fiscal Year Final Research Report Summary

Effects of retinoic cid on adhesion molecules involving desmosome and hemidesmosome in gingival epithelial cells

Research Project

Project/Area Number 12671778
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionIwate Medical University

Principal Investigator

HATAKEYAMA Setsuko  Iwate Medical University, School of dentistry, Lecturer, 歯学部, 講師 (70048495)

Project Period (FY) 2000 – 2002
KeywordsGingival epithelium / desmosome / hemidesmosome / retinoic acid / desmoglein / desmocollin / elecron microscopy / cytokeratin
Research Abstract

Although retinoic acid was reported to compromise desmosome expression in the human epidermis through downregulation of desmosomal cadherins, it is unclear whether retinoic acid inhibits the formation of desmosomes and/or hemidesmosomes in oral epithelia. Whether retinoic acid disintegrated cell-cell and cell-matrix adhesions of oral epithelia was investigated with stratified gingival epithelial (GE1) cells. Ultrastructurally, retinoic acid-treatment (1 μM, 5-10 days) drastically reduced the number of desmosomes and completely destroyed hemidesmosomes. Keratinocyte phenotypes disappeared, accompanied by loss of tonofilaments. The transcripts of desmoglein 1 (ANOVA, P<0.01), desmocollin 1 (P<0.05), and keratin 13 (P<0.05) were significantly decreased, but expression of α6 and β4 subunits of integrin, components of hemidesmosomes, were not changed. These results indicated that retinoic acid also disintegrated desmosomes by depriving the cells of desmoglein 1 and desmocollin 1 in the oral mucosal epithelium. The decrease of keratin filaments, the intracellular cytoskeletal attachments to hemidesmosomes, was probably a cause of hemidesmosome loss.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 畠山節子, 他: "レチノイン酸による不死化口腔上皮細胞の接着分子発現調節"口腔組織培養学会誌. 11(In press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatakeyama, S., et al.: "Retinoic acid inhibits the formation of desmosome and hemiodesmosome in multilayered oral mucosal epithelial cells"J Oral Pathol & Med. 31.5. 294 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatakeyama, S., et al.: "Establishment of gingival epithelial cell lines from transgenic mice harboring temperature -sensitive simian virus 40 large T-antigen gene"J Oral Pathol & Med. 30. 296-304 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatakeyama, S., et al.: "Retinoic acid inhibits the formation of desmosome in oral mucosal epithelial cells"J Dent Res (IADR abstracts). 80. 775 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohara-Nemoto, Y., Hatakeyama, S., et al.: "Gingival epithelial responses to lipopolysaccharide through membrane CD14"J Dent Res (IADR abstracts). 80. 578 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 畠山節子 他: "マウス歯肉上皮由来不死化細胞株の樹立"口腔組織培養学会誌. 9. 21-30 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatakeyama, S., et al.: "Retinoic acid inhibits the formation of desmosome and hemidesmosome in multilayererd oral mucosal epithelial cells"J Oral Pathol & Med. 31. 294 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatakeyama, S., et al.: "Establishment of gingival epithelial cell lines from transgenic mice harboring temperature -sensitive simian virus 40 large T-antigen gene"J Oral Pathol & Med. 30. 296-304 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatakeyama, S., et al.: "Retinoic acid inhibits the formation of desmosome in oral epithelial cells"J Dent Res (IADR abstracts). 80. 775 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohara-Nemoto, Y., Hatakeyama, S., et al.: "Gingival epithelial responses to lipopolysaccharide through membrane CD14"J Dent Res (IADR abstracts). 80. 594 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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