2001 Fiscal Year Final Research Report Summary
Analysis of molecular biology in growth snd invasion of oral squamous cell carcinoma
| Project/Area Number |
12671782
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
TACHIKAWA Tetsuhiko Showa University, School of Dentistry, Professor, 歯学部, 教授 (10085772)
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| Co-Investigator(Kenkyū-buntansha) |
AIDA Tadateru Showa University, School of Dentistry, Assistant, 歯学部, 助手 (10307051)
IRIE Taro Showa University, School of Dentistry, Assistant Professor, 歯学部, 講師 (00317570)
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| Project Period (FY) |
2000 – 2001
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| Keywords | squamous cell carcinoma / keratinocvte / progression / carcinogenesis / doc-1 / apoptosis / dysplasia / cvclin |
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| Research Abstract |
Disruption of the homeostatic balance between proliferation and apoptosis is widely believed to contribute to human oral carcinogenesis. Recently, p12doc-1 is a growth suppressor that negatively regulates cyclin-dependent kinase 2 (CDK 2) activities. Expression of p2ldoc-l is reduced and /or lost in tumor tissue.
Using the Syrian hamster oral cancer model, we examined normal, hyperplastic, dysplastic and malignant oral epithelium for the fraction of apoptotic, proliferating and p21doc-1 expressing keratinocytes using TUNEL assay, as well as PCNA and p21doc-1 immunostaing, respectively. The percentage of TUNEL positive cells progressively increased from normal to dysplastic cells increased progressively through hamster oral malignant progression. The overall ratio of apoptotic to proliferating keratinocyte remains similar until the transition between dysplastic and malignant epithelium, where the ratio is markedly reduced. P21doc-1 labeling demonstrated a similar expression pattern. This study demonstrates that the apoptosis, proliferation and expression of p21doc-1 reflect alterations reported during human oral carcinogenesis.
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Research Products
(12 results)
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[Publications] Shintani, S., Ohyama, H., Zang, X., Mcbrige, J., Matsuo, K., Tsuji, T., Hu, G., Kohno, Y., Lerraan, M., Todd, R. and Wong, D: "p21(Doc-1)is a novel cyclin-dependent kinase 2-associated protein."Mol. Cell. Biol. 20. 6300-6307 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tachikawa, T., Hasegawa, I., Tsuchiya, R., Ymamamoto, G., Maeda, Y., Irie, T., Tamura, Y., Hamaoka, M., Nemori, R. and Bando, T: "A new method of in situ zymography for matrix metalloprotenase of oral squamous cell carcinoma."Oral Oncology. 7. 471-480 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tsuji, T., Usui, S., Aida, T., Tachikawa, T., Hu, G., Sasaki, A., Matsumura, T., Todd, R.and Wong, T: "Induction of epithelial differentiation and DNA demethylation in hamster malignant oral keratinocyte by ornithin decarboxylase antizyme."Oncogene. 20. 24-33 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shintani, S., Mihara, M., Terakado, N., Nakahara, Y., Matsumura, T., Kohno, Y., Ohama, H., McBride, J., Kent, R., Todd, R., Tsuji, T and Wong, D.: "Reduction of pl2doc-l expression is a negative progrestic indicator in patient with surgically resected.oral squamous cell carcinoma."Clin. Cnacer Res.. 7. 2777-2782 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Tachikawa, T., Irie, T., Saito, Y., Aida, T., Yamamoto, G. and Tsuchiya, R: "A cell diversity of oral squamous cell carcinoma in diagnosis of malignancy"J. Jpn. Soc. Oral Tumor. 13(in press). (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Kohno, Y., Patel, V., Kirn, Y., Tsuji, T., Chin, B., Sun.M., Donoff, B., Kent, R., Wong, D. and Todd, R.: "Apoptosis, proliferation and pl2doc-l profiles in normal, dysplastic and malignant squamous cell epithelium of the Syrian hamster cheek pouch model."Oral Oncology. (in press). (2002)
Description
「研究成果報告書概要(欧文)」より
