2001 Fiscal Year Final Research Report Summary
Functional Development of Nociceptors in the Oral Mucosa
| Project/Area Number |
12671800
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Nagasaki University (2001)
Tokyo Medical and Dental University (2000) |
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Principal Investigator |
TODA Kazuo Oral Physiology, Nagasaki University, Prof., 歯学部, 教授 (80134708)
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| Project Period (FY) |
2000 – 2001
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| Keywords | nociceptor / subtype / development / jaw-nerve preparation / high threshold / A-delta fiber / C-fiber / mechanonociceptor |
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| Research Abstract |
Functional Development of the oral mucosal nociceptors was investigated using in vitro Jawnerve preparation which was recently developed by ours. A total of 36 male Wistar albino rats weighing 250-350 g were tested. The animals were housed in conventional acrylic cages with soft bedding under a 14 h light/10 h dark cycle. Only one recording was obtained from each animal and the animal was sacrificed immediately after experiment with an intracardiac injection of thiamylal sodium (100 mg/kg, Isozol^【○!R】, Yoshitomi Pharmacy, Osaka, Japan). All experiments were approved by the Animal Care Welfare Committee Nagasaki University.
In the oral mucosa, four subtypes of nociceptors were found: A-delta high threshold mechanonociceptor, A-delta polymodal nociceptor, C high threshold mechanonociceptor, C polymodal nocieptor. These nociceptors were maturated by 3-5 weeks after birth. The ratio of polymodal nociceptors innervated by A-delta or C afferent fiber was decreased during development.
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