2002 Fiscal Year Final Research Report Summary
Induction of tooth differentiation by gene transfection using adenovirus
| Project/Area Number |
12671818
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tsurumi University, School of Dental Medicine |
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Principal Investigator |
OIDA Shinichro Tsurumi University, School of Dental Medicine, Associate Professor, 歯学部, 助教授 (10114745)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANE Akira , 講師 (20166763)
TERASHIMA Tatsuo Tokyo Medical and Dental University, Faculty of Dentistry, Associate Professor, 歯学部, 助教授 (20114770)
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| Project Period (FY) |
2000 – 2002
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| Keywords | BMP receptor / gene transfection / Tooth differentiation / adenovirus |
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| Research Abstract |
To investigate the molecular mechanism of the tooth development, we have examined the specific genes for tooth differentiation and the effect of the gene products on the tooth differentiation using adenovirus vector.
(1) Differentiation of tooth germ
We investigated the several genes for differentiation and reported the results.
(2) Gene transfection to cultured cells (ST2 and C2C12)
To confirm the activity of the used genes, ALK1〜7(receptor of BMP family) and Smad gene were transfected to the cells. Some of them stimulated the BMP signaling.
(3) Gene transfection to mouse tooth germs in organ culture system.
We investigated the effect of ALK1〜7 (constitutive active type) on mouse tooth germ in organ culture system. Some of them differentiated the tooth in early stage as well as BMP ligand.
These results suggest that the family genes are important role in tooth differentiation at early tooth development.
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[Publications] Oida, S., Nagano, T., Yamakoshi, Y., Ando, H., Yamada, M., and Fukae, M.: "Gene expressions of amelogenin and dentin sialophosphoprotein in porcine developing tooth"The proceedings of international symposium of oral science in Tsurumi University School of Dental Medicine. (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Nagano, T., Kanazashi, M., Oida, S., Gomi, K., Arai, T., and Fukae, M.: "Gene expressions of enamel proteins and proteinases involved in their degradation in the tooth germ of porcine developing tooth"The proceedings of international symposium of oral science in Tsurumi University School of Dental Medicine. (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamakosi, Y., Tanabe, T., Oida, S., Hu, C-C., Simmer, J.P., and Fukae, M.: "Calcium binding of enamel proteins and their deriveatives with emphasis on the calcium-binding domain of porcine sheathlin"Arch. Oral Biol.. 46. 1005-1014 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Oida, S., Nagano, T., Yamakoshi, Y., Ando, H., Yamada, M. and Fukae, M.: "Amelogenin gene expression in porcine odontoblasts"J. Dent. Res.. 81. 103-108 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Fukae, M., Tanabe, T., Nagano, T., Ando, H., Yamakoshi, Y., Yamada, M., Simmer, J.P. and Oida, S.: "Odintoblasts enhance the maturation of enamel crystals by secreting EMSP1 at the enamel-dentin junction"J. Dent. Res.. 81. 668-672 (2002)
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「研究成果報告書概要(欧文)」より
