2001 Fiscal Year Final Research Report Summary
Changeinradiation sensitivityby mitochondrial damageand their mechanisms
| Project/Area Number |
12671844
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | Kagoshima University (2001)
National Institute of Radiological Sciences (2000) |
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Principal Investigator |
MAJIMA Hideyuki Kagoshima University, Dental School, Professor, 歯学部, 教授 (60165701)
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| Co-Investigator(Kenkyū-buntansha) |
ANDO Koichi National Institute of Radiological Sciences, Researcher, 研究員 (00159526)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Radiation / Apoptosis / Mitochondria / Sensitivity / Manganese SOD |
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| Research Abstract |
We observed previously that mitochondria DNA-less (Rho 0) cells are much more sensitive to radiation in terms of the radiationsensitivity (colony assay), apoptosis (Hoechst stain), DNA double strand breaks, and chromosome aberrations. An other finding is that Rho 0 cells contain much less amount of manganese superoxidedismutase (MnSOD) in themitochondria. A transfection vector containing MnSOD was made and was transfected to the Rhocells, and the change in apoptosis frequency was observed. The results showed that frequency of apoptosis by X-irradiation was decreased compared to that in the non-transfected cells, suggesting the MnSOD is a factor to determine the radiation sensitivity. These results indicate that changes in mitochondrial factors play an important key-role in determination of the radiation sensitivity. These evidences were also confirmed by transfecting to an other cell line, HLE, a hepatocarcinoma cell line, by transfecting the MnSOD gene containingvectors. These results suggest that change in superoxide levels in mitochondria is an important factor to determine the cellular radiation sensitivity.
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[Publications] Ando K, Furusawa Y, Suzuki M, Nojima K, Majima H, Koike S, Aoki M, Shimizu W, Futami Y, Ogino T, Murayama S, Ikeda H: "Relative Biological Effectiveness of the 235 MeV proton beams at the National Cancer Hospital East"J. Radiat. Res.. 42. 79-89 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Motoori S, Majima HJ, Ebara M, Kato H, Hirai F, Kakinuma S, Yamaguchi C, Ando K, Ozawa T, Nagano T, Tsujii H, Saisho H: "Overexpression of mitochondrial manganese superoxide dismutase protects against radiation-induced cell death in the human hepatocellular carcinoma cell line, HLE"Cancer Res.. 61. 5382-5388 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Yamada,S., Durante,M., Ando,K., Furusawa,Y., Kawata,T., Majima,H., Tsujii,H.: "Complex-type chromosomal exchanges in blood lymphocytes during radiation therapy correlate with acute toxicity"Cancer Lett.. 150. 215-221 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Durante,M., Yamada,S., Ando,K., Furusawa,Y., Kawata,T., Majima,H., Nakano,T., Tsujii,H.: "X-rays vs. carbon-ion tumor therapy: cytogenetic damage in lymphocytes"Int. J. Radiat. Oncol. Biol. Phys.. 47. 793-798 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ando,S., Nojima,K., Ishihara,H., Suzuki,M., Ando,M., Majima,H., Ando,K., Kuriyama,T.: "Induction by Carbon-ion irradiation of the expression of vascular endothelial growth factor in lung carcinoma cells"Int. J. Radiat. Biol.. 76. 1121-1127 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ando,K., Furusawa,Y., Suzuki,M., Nojima,K., Majima,H., Koike,S., Aoki,M., Shimizu,W., Futami,Y., Ogino,T., Murayama,S. and Ikeda,H.: "Relative Biological Effectiveness of the 235 MeV proton beams at the National Cancer Hospital East"J.Radiat. Res.. 42. 79-89 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Motoori,S., Majima,HJ., Ebara,M., Kato,H., Hirai,F., Kakinuma,S., Yamaguchi,C., Ando,K., Ozawa,T., Nagano,T., Tsujii,H. and Saisho,H.: "Overexpression of mitochondrial manganese superoxide dismutase protects against radiation-induced cell death in the human hepatocellular carcinoma cell line, HLE"Cancer Res. 61. 5382-5388 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Zhao,Y., Xue,Y., Oberley,TD., Kiningham,KK., Lin,S., Yen,HC., Majima,H., Hines,J. and Glair,St.D.: "Overexpression of MnSOD suppresses tumor formation by modulation of AP-1 signaling in a multistage skin carcinogenesis model"Cancer Res. 61. 6082-6088 (2001)
Description
「研究成果報告書概要(欧文)」より
