2001 Fiscal Year Final Research Report Summary
Study for enhancement of VEGF production and angiogenesis in human Human oral squamous cell carcinoma cell
| Project/Area Number |
12671926
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SHIOIRI Shigeaki Tokyo Medical and Dental University, Department of Dentistry. Assistant Professor, 歯学部・付属病院, 講師 (90124693)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Ikuo Tokyo Medical and Dental University, Graduate School, Associate Professor, 大学院・医歯学総合研究科, 助教授 (60100129)
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| Project Period (FY) |
2000 – 2001
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| Keywords | squamous cell / Angiogenesis / VEGF / PEDF / Metastasis / hyaluronidase-1 |
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| Research Abstract |
Angiogenesis is an important phenomenon for the growth and metastasis of solid tumors. The present study examined the characterization of angiogenic factors produced by human oral squamous cell carcinoma (oral SCC) cell lines. The conditioned medium of HSC3 with the strongest metastatic ability among the examined lines produced high amount of VEGF and enhanced tube formation of endothelial cells. The mechanisms for enhancing angiogenesis in HSC-3 is due to 1)constitutive expression of high SP-1, 2)production of the factors inducing expression of VEGF receptor. The cells also produced hyaluronidase-1 and produced low molecular hyaluronic acid, which is one of the angiogenic factor. HSC-3 also produced pigmental epithelium-derived factor, which is anti-angiogenic factor (PEDF). The production of PEDF is regulated by VEGF , and as a result the balance between VEGF and PEDF regulates
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