2001 Fiscal Year Final Research Report Summary
Development of pedicled plastic bone flap using cultured osteogenic cells and its application for the reconstruction of mandibular bone defect
| Project/Area Number |
12671945
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SATOMURA Kazuhito University Dental Hospital, The University of Tokushima, Assistant Professor, 歯学部・附属病院, 講師 (80243715)
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| Co-Investigator(Kenkyū-buntansha) |
SHIKINAMI Yasuo Takiron Co, Ltd., Medical Division, Director, メディカル事業部, 部長
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| Project Period (FY) |
2000 – 2001
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| Keywords | Bone reconstruction / Bone substitute / Bone marrow stromal cell / Osteoblast / Cell culture / Biomaterial / Regenerative medicine |
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| Research Abstract |
Establishment of ideal reconstruction method for mandibular bone defects resulting from various diseases is important to improved quality of life. From this point of view, novel reconstruction method using cultured osteogenic cells should be developed. In this study, we tried to establish new reconstruction method for bone defects using pedicled plastic bone flap with membrane-type biomaterial and cultured osteogenic cells. The result obtained from the present study is as follows:
1. To decide on the best carrier for the transplantation of osteogenic cells, the capacity of type I collagen, hydroxyapatite granules and tricalcium phosphate-containing apatite to support the bone formation by human osteogenic cells was investigated. This experiment showed that three-dimensional type I collagen sponge was the best carrier for die transplantation of cultured osteogenic cells.
2. To elucidate the effect of glucocorticoid on the bone forming capacity of human osteogenic cells, human osteoblasts
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treated with/without dexamathasone were subcutaneous or intramuscularly transplanted into immunodeficient SCID mice using type I collagen as a carrier. This experiment revealed that dexemethasone strongly enhanced the bone forming capacity of human osteoblasts in vivo. This suggested that glucocorticoid might be a potent stimulator for bone formation in pedicled bone flap.
3. Mouse osteogenic cells treated with dexamethasone were transplanted around various arteries with type I collagen and membrane-type biomaterial. Histological observation of transplants showed that 32 of 37 osteogenic cells-containing transplants formed a vasucularized bone island. Mature transplants consisted of well-developed lamellar, corticocancellous bone whose osteocytes were derived from the grafted osteogenic cells. Transplants formed as early as 4 weeks and remained stable in size as late as 108 weeks.
Taken together, this study suggested the possibility of novel pedicled hybrid bone flap using osteogenic cells and membrane-type biomaterial Less
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