2002 Fiscal Year Final Research Report Summary
Adhesion molecule expression of gingival crevice epithelium against PMN infiltration
| Project/Area Number |
12672043
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Nippon Dental University |
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Principal Investigator |
KATSURAGI Hiroaki Nippon dental University, School of Dentistry at Niigata, Microbiology, Associate professor, 新潟歯学部, 助教授 (70224483)
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| Project Period (FY) |
2000 – 2002
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| Keywords | ICAM-1 / Integrin / Gingival crevice epithelium / PMN / Cytokine |
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| Research Abstract |
Discovery of the adhesion molecule expression on gingival epithelium, immunohistochemistry methods and air-liquid interface organotypic culture were performed in vivo and in vitro study. Consequently, both in vivo and in vitro assay, in gingival crevice epithelium expressed ICAM-1 in composition, and can consider that this is the maximum major candidate of the counter receptor against PMN infiltrations. On the other hand, the expression of serectins, such as CD62e and CD62p, was not shown in the gingival crevice epithelium, but the expression of alpha 6- beta 1 integrin.was shown the gingival crevice epithelium. CD62p and CD 106 expression were shown strongly at the vas capillare under the gingival epithelium. The interaction of PMN and gingival crevice epithelium, the epithelium was damaged by periodontopathic bacteria phagocytosed PMNs which produced of high oxygen radicals, erastase, TNFalpha, and MMP- 8 and 9. This cytotoxic effect of the epithelium from PMN was not inhibited at the individual each inhibitor against oxygen radicals, erastase, TNFalpha, and MMP- 8 and 9. So, these result suggested that this cytotoxic effect was total effect of PMN-bacteria interaction reactivity.
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Research Products
(6 results)
