2001 Fiscal Year Final Research Report Summary
Function of oxidized protein hydrolase in cells
| Project/Area Number |
12672123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
KIYOMI Kikugawa School of Pharmacy,Tokyo University of Pharmacy and Life Science,Professor, 薬学部, 教授 (90120146)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Oxidized protein / Red cell membrane / Oxidized protein hydrolase(OPH) / Cultured cell / Proteasome / プロテアソーム |
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| Research Abstract |
We have found the presence of oxidized protein hydrolase in the membranes in human erythrocytes. In this study, the following investigation was done.
(1)The enzyme was isolated from human erythrocytes, characterized as a serine protease, and named oxidized protein hydrolase (OPH).
(2)Cleaving sites of oxidized protein by OPH were clarified.
(3)OPH was distributed in a wide variety of tissues and cells
(4)COS-7 cells with highly expressing OPH(COS-7-OPH) were prepared, in which OPH was present uniformly in the cytosol.
(5)COS-7-OPH was highly resistant to oxidative stress, indicating that OPH protected the cells from oxidative stress.
(6)COS-7-OPH was resistant to oxidative stress in the presence of MG-132 that specifically inhibits proteasome. Involvement of Hsp70 in the activity of OPH was suggested.
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