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2001 Fiscal Year Final Research Report Summary

The Study of Neuroprotection and Regeneration on Neurodegenerative Flatworm model using inverteverate Planarian

Research Project

Project/Area Number 12672136
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKyoto Pharmaceutical University

Principal Investigator

KITAMURA Yoshihisa  Pharmarmaceutical Sciences, Neurobiology, Kyoto Pharmaceutical University, Associate Professor, 薬学部・病態生理学, 助教授 (60195295)

Project Period (FY) 2000 – 2001
KeywordsPlanarian (Dugesia japonica) / Invertebrate Flatworm / Rotenone / MPTP / Dopaminergic Reagents / Caspase Inhibitors / Neurodegeneration / Novel Parkinsonian Flatworm Model
Research Abstract

Recently, it has been shown that rotenone and 1-methyl-4-phenylpyridinium (MPP^+, a metabolite of MPTP), which inhibit mitochondrial complex I, are useful tools for parkinsonian models in vertebrates such as primates and rodents. Planarian, an invertebrate flatworm, has a high potential for regeneration, and dopamine plays a key role in its behavior. We examined a cloned planarian, the GI strain from Dugesia japonica Planarians that were treated with rotenone or MPTP underwent autolysis and individual death in a concentration- and time-dependent manner. In addition, these effects induced by rotenone or MPTP were inhibited by several antiparkinsonian drugs and caspase inhibitors. These results suggest that exposure to rotenone and MPTP causes dopaminergic dysfunction, parkinsonism-like symptoms and caspase-dependent death even in a flatworm, planarian. Since planarian is a lower invertebrate than fruit fly (Drosophila melanogaster) and nematode (Caenorhabditis elegans), common functions of dopaminergic neurons and caspase-dependent pathway(s) may exist from lower invertebrates to mammals. However, DNA degradation, rather than DNA fragmentation, occurred in planarian. Thus, planarian treated with rotenone or MPTP is a novel parkinsonian flatworm model.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Yoshihisa Kitamura: "The parkinsonian models : Invertebrates to mammals"Japanese Journal of Pharmacology. 84・3. 237-243 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihisa Kitamura: "Decrease of tyrosine hydroxlase-, α-synuclein and parkin-positive neurons in the substantia nigra of MPTP-treated C57BL/6N mice"Biogenic Amines. 16・2. 127-136 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jun-ichi Kakimura: "Release and aggregation of cytochrome c and α-synuclein are inhibited by the antiparkinsonian drugs, talipexole and pramipexole"European Journal of Pharmacology. 417・1-2. 59-67 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jun-ichi Kakimura: "BiP/GRP78-induced production of cytokines and uptake of amyloid-β(1-42) peptide in microglia"Biochemical and Biophysical Research Communications. 281・1. 6-10 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihisa Kitamura, et al.: "The parkinsonian models: Invertebrates to mammals."Jpn. J. Pharmacol.. 84 (3). 237-243 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshihisa Kitamura, et al.: "Decrease of tyrosine hydroxylase-, α-synudein and parkin-positive neurons in the substantia nigra of MPTP-treated C57BL/6N mice"Biogenic Amines.. 16 (2). 127-136 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jun-ichi Kakimura, et al.: "Release and aggregation of cytochrome c and α-synuclein are inhibited by the antiparkinsonian drugs, talipexole and pramipexole"Eur. J. Pharmacol.. 417 (1-2). 59-67 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jun-ichi Kakimura, et al.: "BiP/GRP78-induced production of cytokines and uptake of amyloid-β(1-42) peptide in microglia"Biochem. Biophys. Res. Commun.. 281 (1). 6-10 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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