2001 Fiscal Year Final Research Report Summary
DEVELOPMENT OF NOVEL ASSAY SYSTEM FOR ANTI-ALLERGIC COMPOUNDS FROM NATURAL SOURCES AND APPLICATION
| Project/Area Number |
12672166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
医薬分子機能学
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| Research Institution | Mukogawa Women's University |
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Principal Investigator |
ISHIGURO Kyoko Mukogawa Women's University, School of Pharmacy, Associate Professor, 薬学部, 助教授 (70151363)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIGURO Masaji Suntory Institute for Boirganic Research, Chief Scientist, 部長研究員 (10280687)
OKU Hisae Mukogawa Women's University, School of Pharmacy, Assistant, 薬学部, 助手 (90281518)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Blood flow monitiring / PAF / Anti-anaphylaxis assay method / Anaphylaxis / anti-allergy / compound 48 / 80 |
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| Research Abstract |
Monitoring the blood flow of unanesthesized mice was found to be a reliable and effective method for studying their Hen egg-white lysozyme (HEL)-specific anaphylaxis, in addition to the known method of monitoring blood pressure. This method is convenient for searching for both anaphylaxis and anti-anaphylactic substances from natural products. Using this system, we estimated the anti-anaphylactic effects of the 35% ethanol extract (IB) of petals of Impatiens balsamina L., as well as those of anti-allergic agents currently used. Flavonoids and 1, 4-naphthoquinones from IB significantly inhibited the decrease of blood flow. We also found that PAF and serotonin participate in decreasing the blood flow, but histamine does not.
The discovery of the blood flow lowering by single HEL-sensitization led to design of the assay system for screening of allergy inhibitory substances guided by blood flow lowering. By employing this assay method, we are able to clarify that the petals of Impatien textori Miq. suppress a decrease in blood flow.
The CHCl3-soluble part (HJ) of the MeOH extract from Hypericum japonicum afforded new bixanthones, jacarelhyperols A (1) and B (2). These compounds and HJ showed significant inhibitory effects against PAF-induced hypotension by an in vivo blood pressure method, which can reveal pharmacological action of the test material. The binding mechanism of the above compounds on PAF receptor is under investingation.
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