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2002 Fiscal Year Final Research Report Summary

Synthesis, metabolism andfibrillogenesis of serum amyloid A bymonocytes or macrophages

Research Project

Project/Area Number 12672251
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionJUNTENDO UNIVERSITY

Principal Investigator

YAMADA Toshiyuki  JUNTENDO UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF CLINICAL PATHOLOGY, ASSOCIATE PROFESSOR, 医学部, 助教授 (50211636)

Project Period (FY) 2000 – 2002
Keywordsserum amyloid A / amyloidosis / monocyte / macrophage / amyloidosis / inflammation / fibrillogenesis / glucocorticoid
Research Abstract

Serum amyloid A (SAA) is a precursor of AA proteins, the main constituent of reactive amyloid deposits. Macrophages play a central role in AA fibrillogenesis and the cells can produce SAA. In this study, using a monocytic leukemia cell line THP-1 as a model of macrophages, several features in SAA synthesis by monocytes/macrophages were examined. The cells secrete SAA mainly by IL-1 and glucocorticoid is critical for SAA production. Indeed, in the presence of glucocorticoid, THP-1 can produce SAA under the culture condition using common media containing calf serum. These features are quite different from those of hepatocytes, the main producer of SAA. These results imply that SAA may be produced by macrophages, and possibly other non-hepatic tissues under the physiological conditions although the amount is not comparable with that from the liver at inflammation. The possibility that macrophages utilize self-produced SAA in fibril formation when glucocorticoid is used therapeutically cannot be ruled out. Next, whether or not THP-1 cells can make amyloid fibrils when SAA proteins are added to its culture media was assessed. Several conditions were attempted, but failed to yield congo red positive materials. THP-1 cells were highly aggregated by the addition of SAA, it may represent the feature of SAA as chemoattractant. For fibrils formation, further alteration of experimental conditions may be necessary.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 山田 俊幸: "Serum amyloid A secretion from monocytic leukaemia cell line THP-1 and cultured human peripheral monocytes"Scandinavian Journal of Immunology. 52. 7-12 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 俊幸: "Relative serum amyloid A (SAA) values : the influence of SAA1 genotypes and corticosteroid treatment in Japanese patients with rheumatoid arthritis"Annales of Rheumatic Diseares. 60. 124-127 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 俊幸: "Further characterization of serum amyloid A4 as a minor acute phase reactant and a possible nutritional indicator"Clinical Chemistry and Laboratory Medicine. 39. 7-10 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Murai T: "Ferofibrate inhibits reactive amyloidosis in mice"Arthritis and Rheumatism. 46. 1683-1688 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 俊幸: "An allele of serum amyloid A1 associated with amyloidosis in both Japanese and Caucasians"Amyloid. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada T, Okuda Y, Takasugi K, Itoh K, Igari J: "Relative serum amyloid A (SAA) values : the influence of SAA1 genotypes and corticosteroid treatment in Japanese patients with rheumatoid arthritis"Ann Rheum Dis. 60. 124-127 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada T, Miyake N, Itoh K, Igari J: "Further characterization of serum amyloid A4 as a minor acute phase reactant and a possible nutritional indicator"Clin Chem Lab Med. 39. 7-10 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada T, Mukaiyama I, Miyake N, Igari J: "Measurement of cystatin C in cerebrospinal fluid"Rinsho Byori. 50. 613-617 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murai T, Yamada T, Miida T, Arai K, Endo N, Hanyu T: "Fenofibrate inhibits reactive amyloidosis in mice"Arthritis Rheum. 46. 1683-1688 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko K, Tsuchiya K, Kawamura R, Ohtomo Y, Shimizu T, Yamashiro Y, Yamada T, Yamauchi K, Kitagawa T: "Low prevalence of hypercalciuria in Japanese children"Nephron. 91. 439-443 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Fukushima K, Ogawa H, Kitayama T, Yamada T, Naito H, Funayama Y, Matsuno S, Sasaki I: "Epithelial induction of serum amyloid A in experimental mucosal inflammation"Dig Dis Sci. 47. 1438-1446 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada T, Okuda Y, Takasugi K, Wang L, Marks D, Benson MD, Kluve-Beckerman B: "An allele of serum amyloid A1 associated with amyloidosis in both Japanese and Caucasians"Amyloid : J Prot Fold Dis. 10 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada T, Sotoh T: "Changes in individual serum beta2-microglobulin in regular hemodialysis"Ckinical Nephrology. (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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