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2001 Fiscal Year Final Research Report Summary

STUDIES ON REGULATION OF STEROID HORMONE SYNTHESIS AND METABOLISM IN FETO-PLACENTAL UNIT

Research Project

Project/Area Number 12672258
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKobe Pharmaceutical University

Principal Investigator

KOBAYASHI Yoshiharu  KOBE PHARMACEUTICAL UNIVERSITY, FACULTY OF PHARMACY, ASSOCIATE PROFESSOR, 薬学部, 助教授 (20133647)

Co-Investigator(Kenkyū-buntansha) FUJINAMI Aya  Kobe Pharmaceutical University, Faculty of Pharmacy, Assistant, 薬学部, 助手 (30299086)
TAGAWA Noriko  Kobe Pharmaceutical University, Faculty of Pharmacy, Assistant, 薬学部, 助手 (00206905)
Project Period (FY) 2000 – 2001
Keywords16-dehydropregnenolone / EIA / mother-placenta / pregnenolone / steroid metabolism / 17-hydroxylation / 16-hydroxylation / GC / MS
Research Abstract

We had identified 16-dehydropregnenolone sulfate (16-DHPS) as a novel steroid in serum from neonate. We also revealed that this steroid is synthesized according to the following process : Pregnenolone sulfate (PregS)→16-hydroxy-PregS (16-OH-PregS) →16-DHPS. However, the physiological significance of 16-DHPS is unclear. The aim of this study is to investigate whether this metabolic pathway regulate the known steroid pathway, PregS→17-hydroxy-PregS (17-OH-PregS)→dehydroepiandrosterone sulfate (DHEAS)→16-OH-DHEAS, because these pathways use common substrate, PregS. Firstly, we developed methods for the measurement of these steroids : PregS, 16-OH-PregS, 16-DHPS, 17-OH-PregS, DHEAS and 16-OH-DHEAS by GC/MS. Methods for the measurement of their unconjugated steroids by EIA were also established (Biol. Pharm. Bull. 24,867 (2001). Using these methods, serum concentrations of these steroids were determined in the samples from pregnant women. Key step of the above two pathways is 17-hydroxylation and 16-hydroxylation of Preg(S). 17- and 16-hydroxylase activities were obtained by the ratio of product/substrate concentration that is 17-OH-Preg(S)/Preg(S) ratio and 16-OH-Preg(S)/Preg(S) ratio, respectively. As a result, the 17-hydroxylase activities were decreased according to the course of pregnancy. Inversely, the 16-hydroxylase activities were increased at term compared with those at the first trimester of pregnancy. There were no significant changes of the product/substrate ratios for the other steroids in the above pathway. Present data suggest that 17-hydroxylation of Preg(S) is to be regulated by 16-hydroxylation of Preg(S).

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Noriko Tagawa, Aya Fujunami, Nobuyuki Amino, Yoshiharu Kobayashi et al.: "Heterogeneous enzyme immunoassay for serum androstenediol"Clin.Chim.Acta. 296. 193-201 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noriko Tagawa, Kayoko Saiki, Yoshiharu Kobayashi: "Development of an enzyme immunoassay for serum 16-dehydropregnenolone"Biol.Pharm.Bull.. 24. 867-871 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noriko Tagawa, Aya Fujinami, Yoshiharu Kobayashi et al.: "Heterogeneous enzyme immunoassay for serum androstenediol"Clin. Chim. Acta. Vol.296 No.1, 2. 193-201 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriko Tagawa, Kayoko Saiki, Yoshiharu Kobayashi: "Development of an enzyme immunoassay for serum 1 6-dehydropregnenolone"Biol. Pharm. Bull.. Vol.24 No.8. 867-871 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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