2002 Fiscal Year Final Research Report Summary
Ecological method to control methicillin resistant Staphylococcus aureus
| Project/Area Number |
12672280
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎・地域看護学
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
KAKINOHANA Shige University of the Ryukyus, Faculty of Medicine Associate Professor, 医学部, 助教授 (50274890)
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| Co-Investigator(Kenkyū-buntansha) |
IWANAGA Masaaki University of the Ryukyus, Faculty of Medicine Professor, 医学部, 教授 (00112384)
OWAN Tomoko University of the Ryukyus, Faculty of Medicine Associate Professor, 医学部, 助教授 (90295311)
UEMURA Emiko University of the Ryukyus, Faculty of Medicine Assistant Professor, 医学部, 講師 (00223503)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Staphylococcus aureus / nosocomial infection / carrier / drug resistant |
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| Research Abstract |
Methicillin resistant Staphylococcus aureus (MRSA) as a causative agent of nosocomial infection has been notorious in the past 2 decades, and now control the pathogen in the hospital is a pressing problem in the world. The present study was planned to find a method to control MRSA not depending on anti-microbial chemotherapy but depend on an ecological method using some environmental organisms, and also the study was aimed at cultivating nurse specialists for infection control. Examinations of nasal vestibulum for the nurses working at the hospital revealed that about 40% of the nurses carried S. aureus and about 25% of the carriers had MRSA. In three times examinations every 2 months, it was clarified that there were constant carriers, constant non-carriers, and intermittent carriers.
Environmental organisms were collected and each organisms was examined for the inhibition of MRSA. Some organisms inhibited the growth of MRSA on an agar plate and in mixed culture in broth. One strain i-1 markedly inhibited the growth of MRSA. The inhibitory activity was examined on the mouse skin from which the hair was removed. The skin was colonized by MRSA for 10 to 14 days after inoculation but GPR strain i-1 disappeared within a few days. In 2 groups of mice, one inoculated MRSA, another inoculated MRSA and i-1, the period of colonization by MRSA was almost the same. GPR i-1 strain produced MRSA inhibitor in the culture supernatant. The inhibitor was concentrated, applied on the mouse skin, and the MRSA was inoculated. However, no inhibitory effect was found. In conclusion, we found many strains of environmental Gram positive rods which inhibited the growth of MRSA in vitro. However, it was not active on the animal skin surface. The inhibitor should be purified to investigate whether it can be used as anti-microbial drug or not.
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