2001 Fiscal Year Final Research Report Summary
A study on immunomodulatory action by chronic exercise and gene expression of immunoregulatory cytokine.
| Project/Area Number |
12680045
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
体育学
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
SUGIURA Haruo Department of Pharmaceutical Science, Gifu Pharmaceutical University, Assistant, 薬学部, 助手 (40187646)
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| Project Period (FY) |
2000 – 2001
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| Keywords | marcophage / T-cell / cytokine / mRNA / chronic exercise |
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| Research Abstract |
This study was designed to evaluate the effects of 8-week voluntary running exercise on cytokines production and gene expression of immunocompetent cell. Seven-week-old male BALB/c inbred mice were divided into two groups : a group given voluntary exercise (exercise group), and the other, a non-exercise group (control group). Exercise consisted of spontaneous running in wheels for 3' days per week over 8 weeks. The levels of nitric oxide, interleukin (IL)-1β, IL-12 and granulocyte-macrophage colony stimulating factor (GM-CSF) production by lipopolysaccharide (LPS)-stimulated peritoneal macrophages from the exercise group were significantly higher than that in the control group. In comparison with the mRNA concentration of the control group, voluntary exercise increased the expression levels of iNOS, IL-12 and GM-CSF mRNA in the peritoneal macrophages. In the exercise group, IL-2 production of splenocytes stimulated by concanavalin A (Con A) was significantly higher than that in the control group. Interferon-γ production of splenocytes stimulated by Con A in the exercise group was higher than that in the control group however ; the difference was not statistically significant. The expression level of IL-2 mRNA in the exercise group was significantly higher than that in the control group. On the other hand, the expression level of IL-10 mRNA in the exercise group was significantly lower than that in the control group. These results suggest that 8-week voluntary running exercise effectively enhanced macrophage arid lymphocyte functions in mice. Moreover, the in vitro production of IL-2 and IL-10 suggests that chronic exercise may alter the balance of Th1 and Th2 lymphocytes.
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