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2003 Fiscal Year Final Research Report Summary

Analysis of Behavior of Boron Compounds for Neutron Capture Therapy in Animal Organism by Nuclear Magnetic Resonance

Research Project

Project/Area Number 12680507
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nuclear engineering
Research InstitutionShinsyu University

Principal Investigator

YOSHINO Kazuo  Shinsyu University, Department of Chemistry, Assoc.Prof., 理学部, 助教授 (70143964)

Co-Investigator(Kenkyū-buntansha) HIRATSUKA Jyunichi  Kawasaki Medical School, Assoc.Prof., 助教授 (30192298)
Project Period (FY) 2000 – 2003
Keywordsp-BPA / Melanoma / Boron Neutron Capture Therapy / Melanin / Melanin Precursor / DHI / DHICA / ^<11>B-NMR
Research Abstract

Boron Neutron Capture Therapy (BNCT) is the therapy which utilize the nuclear fission energy to destroy the cancer by irradiation of neutron to the cancer part, where ^<10>B is previously selectively accumulated. The fission reaction is that ^<10>B + n-^7Li + a. At present, p-Boronophenylalanine (p-BPA) is used to accumulate ^<10>B selectively into malignant melanoma. In melanoma melanin synthesis is accentuated, and in the melanin synthesis organism, DOPA, DHI, DHICA, which are melanin precursors, exist in large quantities. By our ^<11>B-NMR study of aqueous solutions, it has become apparent that p-BPA complexes with these melanin precersors, and when the complexes are oxidized p-BPA is decomposed into boric acid and tyrosine. We have considered that the complex formation and decomposition correspond to the ^<10>B accumulation and release mechanism respectively.
In this study, using hamster melanoma, cell organelles which contain melanin precursors were fractionated, and p-BPA was addi … More ng to each fractions, and the interactions between p-BPA and melanin precursors were investigated. Two kinds of fractions were prepared, that is, the small granule fractions(SGF), which contain many coated vesicles (CV) where melanin precursors are abundant and the large granule fractions(LGF), which contain premelanosome and melanosome, in both premelanosome and melanosome melanin polymers are abundant. Into the both fractions p-BPA was added and ^<11>B-NMR were measured. In SGF-p-BPA system, two hours after the mixing, a signal was appeared. The signal seems to be due to the p-BPA-melanin precursors complex. The signal increased with time and 8 hours after the mixing, the signal became a broad signal. In LGF p-BPA system, such signal was not observed.
Into the melanoma bearing hamsters p-BPA-fructose complex was administrated. At 2 and 10 hours after administrations, melanoma of hamsters were fractionated, and ^<11>B-NMR was measured. The peak of p-BPA-melanin precursors was not observed in any samples, but the peak of boric acid was observed in all samples.
By ^<11>B-NMR measurements, the following results were obtained. Melanin is divided into two types, i. e., pheomelanin and eumelanin. p-BPA inhibits both melanin formations. p-BPA more complexes with monosaccharides than m-BPA. p-BPA complexes with dopamine. Less

  • Research Products

    (24 results)

All 2003 2002 2001 2000

All Journal Article (22 results) Book (2 results)

  • [Journal Article] ^<11>B NMR Study of p-Carboxybenzeneboronic Acid Ions for Complex Formation with Some Monosaccharides2003

    • Author(s)
      T.M.Bulbul Islam, K.Yoshino, A.Sasane
    • Journal Title

      Anal.Sci. 19

      Pages: 455-460

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Boron Neutron Capture Therapy (BNCT) for Malignant Melanoma with Special Reference to Absorbed Doses to the Normal Skin and Tumor2003

    • Author(s)
      H.Fukuda, J.Hiratsuka, T.Kobayashi, K.Yoshino, H.Karashima, K.Turu, K.Araki, Y.Mishima, M.Ichihashi
    • Journal Title

      Australas.Phys.Eng.Sci.Med 26(3)

      Pages: 78-84

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ^<11>B NMR Study of p-Carboxybenzeneboronic Acid Ions for Complex Formation with Some Monosaccharides2003

    • Author(s)
      T.M.Bulbul Islam, K.Yoshino, A.Sasane
    • Journal Title

      Anal Sci. 19

      Pages: 455-460

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Boron Neutron Capture Therapy (BNCT) for Malignant Melanoma with Special Reference to Absorbed Doses to the Normal Skin and Tumor2003

    • Author(s)
      H.Fukuda, J.Hiratsuka, T.Kobayashi, K.Yoshino, H.Karashima, K.Turu, K.Araki, Y.Mishima, M.Ichihashi
    • Journal Title

      Australas.Phys.Eng.Sci.Med. 26(3)

      Pages: 78-84

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Measurement of Optical Purity of p-BPA-Tyrosine Dipeptide2002

    • Author(s)
      N.Sato, K.Yoshino, K.Kitta, Y.Saitake, J.Hiratsuka, M.Ichihashi
    • Journal Title

      Peptide Science 2001(H.Aoyagi ed), The Japanese Peptide Society

      Pages: 245-248

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ^1H and ^<11>B NMR study of p-Toluene Boronic Acid and Anhydride2002

    • Author(s)
      T.M.Bulbul Islam, K.Yoshino, H.Nomura, T.Mizuno, A.Sasane
    • Journal Title

      Anal.Sci. 18

      Pages: 363-366

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Measurement of Optical Purity of p-BPA-Tyrosine Dipeptide2002

    • Author(s)
      N.Sato, K.Yoshino, K.Kitta, Y.Saitake.J.Hiratsuka, M.Ichihashi
    • Journal Title

      Peptide Science 2001 (H.Aoyagi ed)(The Japanese Peptide Society)

      Pages: 245-248

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A Further Mechanism Study of Boron Accumulation to and Release from Melanoma with p-Boronophenylalanine2001

    • Author(s)
      K.Yoshino, M.Kamijo, M.Kimura, Y.Mishima, H.Kondoh, M.Kadota, S.Ito, K.Wakamatsu, H.Kakihana
    • Journal Title

      Frontiers in Neutron Capture Therapy(M.F.Hawthorne et al. eds.)(Kluwer Academic/Plenum Publishers, New York)

      Pages: 985-989

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Fluorination of p-Boronophenylalanine2001

    • Author(s)
      K.Yoshino, Y.Inamoto, T.Ido, H.Kakihana, M.Ichihashi, Y.Mishima
    • Journal Title

      Frontiers in Neutron Capture Therapy(M.F.Hawthorne et al. eds.)(Kluwer Academic/Plenum Publishers, New York)

      Pages: 831-834

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Structure Determination of p-Boronophenylalanine-Fructose Complex Using ^1J_<cc> coupling Constants2001

    • Author(s)
      M.Kitazawa, K.Yoshino
    • Journal Title

      Frontiers in Neutron Capture Therapy(M.F.Hawthorne et al. eds.)(Kluwer Academic/Plenum Publishers, New York)

      Pages: 857-861

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Simultaneous Determination of p-Boronophenylalanine and Boric Acid Using ^<11>B-NMR2001

    • Author(s)
      K.Yoshino, Y.Tanaka, H.Kakihana, M.Ichihashi, Y.Mishima
    • Journal Title

      Frontiers in Neutron Capture Therapy(M.F.Hawthorne et al. eds.)(Kluwer Academic/Plenum Publishers, New York)

      Pages: 863-868

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Current Clinical Paradigms in Melanoma BNCT, Leading to Cell Modulation Era Teamed with Active ^<10>B-Biodelivery Systems2001

    • Author(s)
      Y.Mishima, H.Kondoh, K.Yoshino, J.Hiratsuka
    • Journal Title

      Frontiers in Neutron Capture Therapy(M.F.Hawthorne et al. eds.)(Kluwer Academic/Plenum Publishers, New York)

      Pages: 1365-1382

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Fluorination of p-Boronophenylalanine2001

    • Author(s)
      K.Yoshino, Y.Inamoto, T.Ido, H.Kakihana, M.Ichihashi, Y.Mishima
    • Journal Title

      Frontiers in Neutron Capture Therapy (M.F.Hawthorne et al.eds.)(Kluwer Academic / Plenum Publishers New York)

      Pages: 831-834

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Structure Determination of p-Boronophenylalanine-Fructose Complex Using 1^J_<cc> coupling Constants2001

    • Author(s)
      M.Kitazawa, K.Yoshino
    • Journal Title

      Frontiers in Neutron Capture Therapy (M.F.Hawthorne et al.eds.)(Kluwer Academic / Plenum Publishers New York)

      Pages: 857-861

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Simultaneous Determination of p-Boronophenylalanine and Boric Acid Using ^<11>B-NMR2001

    • Author(s)
      K.Yoshino, Y.Tanaka, H.Kakihana, M.Ichihashi, Y.Mishima
    • Journal Title

      Frontiers in Neutron Capture Therapy (M.F.Hawthorne et al.eds.)(Kluwer Academic / Plenum Publishers New York)

      Pages: 863-868

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Current Clinical Paradigms in Melanoma BNCT, Leading to Cell Modulation Era Teamed with Active ^<10>B-Biodelivery Systems2001

    • Author(s)
      Y.Mishima, H.Kondoh, K.Yoshino, J.Hiratsuka
    • Journal Title

      Frontiers in Neutron Capture Therapy (M.F.Hawthorne et al.eds.)(Kluwer Academic / Plenum Publishers New York)

      Pages: 1365-1382

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Thermal Neutron Capture Therapy2001

    • Author(s)
      M.Ichihashi, H.Fukuda, K.Yoshino, J.Hiratsuka, T.Kobayash K.Araki, M.Ueda, K.Tsuru
    • Journal Title

      Diagnosis, and Medical Treatment Policy for Melanoma (Kanehara Co.Ltd.)

      Pages: 176-182

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Selective Uptake of para-Boronophenylalanine Increases in Amelanotic Melanoma Cells Transfected by the Tyrosinase Gene2000

    • Author(s)
      J.Hiratsuka, H.Kondoh, T.Tsuboi, K.Yoshino, Y.Imajo, Y.Mishima
    • Journal Title

      Melanoma Research 10

      Pages: 297-302

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Biodistribution of Boron Concentration on Melanoma-bearing Hamsters after Administration of p-, m-, o-Boronophenylalanine2000

    • Author(s)
      J.Hiratsuka, K.Yoshino, H.Kondoh, Y.Imajo, Y.Mishima
    • Journal Title

      Jpn.J.Cancer Res. 91

      Pages: 446-450

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Biodistribution of Boron Concentration on Melanoma-bearing Hamsters after Administration of p-, m-, o-Boronophenylalanine2000

    • Author(s)
      J.Hiratsuka, K.Yoshino, H.Kondoh, Y.Imajo, Y.Mishima
    • Journal Title

      Jpn.J Cancer Res. 91

      Pages: 446-450

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A Further Mechanism Study of Boron Accumulation to and Release from Melanoma with p-Boronophenylalanine New York2000

    • Author(s)
      K.Yoshino, M.Kamijo, M.Kimura, Y.Mishima, H.Kondoh, M.Kadota, S.Ito, K.Wakamatsu, H.Kakihana
    • Journal Title

      Frontiers in Neutron Capture Therapy (M.F.Hawthorne et al.eds.)(Kluwer Academic / Plenum Publishers)

      Pages: 985-989

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Present Situation and Future of the Compounds for Neutron Capture Therapy2000

    • Author(s)
      K.Yoshino, M.Kirihata
    • Journal Title

      Annual Report (1999) for Present Situation with Problems and Development in Future of Neutron Capture Therapy (Association for Nuclear Technology in Medicine)

      Pages: 49-73

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 悪性黒色腫の診断・治療指針2001

    • Author(s)
      市橋正光, 福田寛, 吉野和夫, 平塚純一, 古林徹, 荒木敬司, 上田正登, 鶴顕太
    • Total Pages
      196
    • Publisher
      金原出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 平成11年度中性子捕捉療法の現状と課題および今後の展開に関する調査報告書2000

    • Author(s)
      吉野和夫, 切畑光統
    • Total Pages
      294
    • Publisher
      財団法人 医用原子力技術研究振興財団
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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