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2002 Fiscal Year Final Research Report Summary

Structural Analysis of Ethylene (Plant Hormone) Sensor Protein

Research Project

Project/Area Number 12680666
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SHIRO Yoshitsugu  RIKEN Harima Institute,Biophysical Chemistry laboratory, Chief Scientist, 生体物理化学研究室, 主任研究員 (70183051)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Hiro  RIKEN, Biophysical Chemistry laboratory,Senior Research Scientist, 生体物理化学研究室, 先任研究員 (80270594)
PAK Sam yong  Yokohama City University Graduate School of Integrated Science,Associated Professor, 大学院・総合理学研究科, 助教授 (20291932)
Project Period (FY) 2000 – 2002
KeywordsEthylene / Plant Hormone / Two-component system / Histidine kinase / Copper (I) complex / XAFS / Sensor Protein
Research Abstract

Ethylene is a plant hormone, which is significantly involved in life cycle of plants. Ethylene exhibits its physiological activities by virtue of binding to its sensor protein, ETR1. ETR1 belongs to the two-component regulatory system in cells, and mainly consists of two functional domain, sensor domain and histidine kinase domain. Most recently, it has been found that the ETR1 sensor domain contains Cu(I). In the present project,we have been trying to obtain the direct evidence that Cu(I) is the ethylene binding site of ETR1. We constructed expression system of the recombinant protein of the ETR1 sensor domain (trans-membrane protein) in E. coli, established it purification procedure, and measured the Cu K-edge XAFS spectra. The spectra is significantly different in the presence and absence of ethylene, showing that ethylene bind to the Cu(I) sitel of ETR1 and causes its coordination structural changes.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] I.Matsunaga, A.Yamada, D.-S.Lee, E.Obayashi, N.Fujiwara, K.Kobayashi, H.Ogura, Y.Shiro: "Enzymatic Reaction of Hydrogen Peroxide-Dependent Proxygenase Cytochrome P450s : Kinetic Deuterium Isotope Effects and Analyses by Resonance Roman Spectroscopy"Biochemistry. 41. 1886-1892 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Saito, E.Ito, K.Hosono, K.Nakamura, K.Imai, T.Iizuka, Y.Shiro, H.Nakamura: "The uncoupling of oxygen sensing, phosphorylation signaling and transcriptional activation in oxygen sensor FixL, and FixJ mutants"Molecualr Microbiology. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.-S.Lee, A.Yamada, H.Sugimoto, I.Matsunaga, H.Ogura, K.Ichihara, S.Adachi, S.-Y.Park, Y.Shiro: "Substrate Recognition and Molecular Mechanism of Fatty Acid Hydroxylation by Cytochrome P450 from Bacillus subtilis : Crystallographic, Spectroscopic and Mutational Studies"J.Biol.Chem.. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Makino, E.Obayashi, N.Homma, Y.Shiro, H.Hori: "YC-1 Facilitates Release of the Proximal His Residue in the NO and CO Complexes of Soluble Guanylate Cyclase"J.Biol.Chem.. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kumita, S.Yamada, H.Nakamura, Y.Shiro: "Chimeric sensory kinases containing O2 sensor domain of FixL, and histidine kinase domain from thermophile"Biochem.Biophys.Acta. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Sawai, N.Kawada, K.Yoshizato, H.Nakajima, S.Aono, Y.Shiro: "Characterization of Heme Environmental Structure of Cytoglobin, A Fourth Globin in Human"Biochemistry. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Shiro, Y.Isogai, H.Nakamura, T.Iizuka: "Molecular Anatomy of Cellular System"編者(I.Endo, T.Kudo, H.Osada, T.Shibata, I.Yamaguchi) Elsevier Science B.V.. 231(189-204) (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Nakamura, K. Saito and Y. Shiro: "Quantitative Measurement of Radioactive Phosphorylated Proteins in Wet Polyacrylamide Gels"Anal. Biochem.. 294. 187-188 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] D.-S. Lee, S.-Y. Park, K. Yamane, E. Obayashi, H. Hori and Y. Shiro: "Structural Characterization of n-Butyl-isocyanide Complexes of Cytochromes P450nor and P450cam"Biochemistry. 40. 2669-2677 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Kudo, N. Takaya, S.-Y. Park, Y. Shiro and H.Shoun: "A Positively Charged Cluster Formed in the Heme-distal Pocket o Cytochrome P450nor Is Essential for Interaction with NADH"J. Biol. Chem.. 276. 5020-5026 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I G. Denisov, S.-C. Hung, K. E. Weiss, M. A. McLean, Y. Shiro, S.-Y. Park, P. M. Champion and S. G. Sligar: "Characterization of the Oxygenated Intermediate of the Thermophilic Cytochrome P450 CYP119"J. Inorg. Biochem.. 87. 215-226 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Kikuchi, S.-Y. Park, H. Miyatake, D. Sun, M.Sato, T. Yoshida and Y. Shiro: "Crystal Structure of Rat Biliverdin Reductase"Nature Strl. Biol.. 8. 221-225 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] D.-S. Lee, A. Yamada, I. Matsunaga, K. Ichihara, S.Adachi, S.-Y. Park and Y. Shiro: "Crystallization and Preliminary X-ray Diffraction Analysis of Fatty-aci Hydroxylase Cytochrome P450BSβ from Bacillus subtilis"Acta Crystal.. D58. 687-689 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Hayashi, T. Matsuo, Y. Hitomi, K. Okawa, A. Suzuki, Y. Shiro, T. Iizuka, Y. Hisaeda and H.Ogoshi: "Contribution of Heme-propionate Side Chains to Structure and Function of Myoglobin: Chemical Approach by Artificially Created Prosthetic Groups"J. Inorg. Biochem.. 91. 94-100 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Sakai,Y. Matsui, T. Kouyama, Y. Shiro and S.Adachi: "Optical monitoring of freeze-trapped reaction intermediates in protein crystals: a microspectrophotometer for cryogenic protein crystallography"J. Appl. Cryst. 35. 270-273 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I. Matsunaga, A. Yamada, D.-S. Lee, E. Obayashi, N. Fujiwara, K. Kobayashi, H. Ogura and Y. Shiro: "Enzymatic Reaction of Hydrogen Peroxide-Dependent Peroxygenase Cytochrome P450s : Kinetic Deuterium Isotope Effects and Analyses by Resonance Raman Spectroscopy"Biochemsitry. 41. 1886-1892 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Saito, E. Ito, K. Hosono, K. Nakamura, K. Imai, T. Iizuka, Y. Shiro and H. Nakamura: "The uncoupling of oxygen sensing, phosphorylation signaling and transcriptional activation in oxygen sensor FixL and FixJ mutants"Molecular Microbiology. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] D.-S. Lee, A. Yamada, H. Sugimoto, I. Matsunaga, H. Ogura, K. Ichihara, S. Adachi, S.-Y. Park and Y.Shiro: "Substrate Recognition and Molecular Mechanism of Fatty Acid Hydroxylation by Cytchrome P450 from Bacillus subtilis: Crystallographic, Spectroscopic and Mutational Studies"J. Biol. Chem.. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R. Makino, E. Obayashi, N. Homma, Y. Shiro and H. Hori: "YC-1 Facilitates Release of the Proximal His Residue in the NO and CO Complexes of Soluble Guanylate Cyclase"J. Biol. Chem.. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Kumita, S. Yamada, H. Nakamura and Y. Shiro: "Chimeric sensory kinases containing O2 sensor domain of FixL and histidine kinase domain from tharmophile"Biochim. Biophys. Acta. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Sawai, N. Kawada, K. Yoshizato, H. Nakajima,S. Aono, and Y. Shiro: "Characterization of Heme Environmental Structure of Cytoglobin, A Fourth Globin in Human"Biochemistry. (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Shiro, Y. Isogai, H. Nakamura and T. Iizuka, Eds. by I. Endo, T Kudo, H. Osada, T. Shibata and I. Yafliaguchi: "Physiological functions and molecular structures of new types of hemoprotelns, Molecular Anatomy of Cellular System V."Elsevier Science B.. 189-204 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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