2001 Fiscal Year Final Research Report Summary
Implications of nitrocathecholamines in Parkinson's disease
| Project/Area Number |
12680760
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Teikyo University |
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Principal Investigator |
NAKAKI Toshio Teikyo University School of Medicine, Department of Pharmacology, Professor, 医学部, 教授 (30164148)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA Nobuko Teikyo University School of Medicine, Department of Pharmacology, Professor, 医学部, 助手 (30317698)
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| Project Period (FY) |
2000 – 2001
|
| Keywords | nitric oxide / catecholamine / neurodegenation / Parkinson's disease / hydrogen peroxide / パーキンソン病 / ドパミン / ノルエピネフリン |
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| Research Abstract |
It was investigated whether or not 6-nitronorepinephrine increased hydrogen peroxide production. 6-Nitronorepinephrine increased it in rat vascular endothelial cells ; at 0 μM, 0-0.2 nmol/60 min/10^5 cells ; 0.3μM, 1-2 nmol/60 min/10^5 cells. Further inceases in the amine concentration was inappropriate because the color of the amine interfered the hydrogen peroxide assay.
Possible apoptosis-eliciting activities of 6-nitronorepinephrine was tested with use of SH-SY5Y cells. Apoptosis was evaluated by released histone from mucleosomes. The amine at a concentration of 300 μM induced apoptosis. 6-1OHDA, a control compound, induced apoptosis at concentrations of 30 and 300 μM.
A neurotoxic pesticide, glufosinate ammonium, was found to be able to stimulate nitric oxide production in rat brain and to induce convulsion. HMDA receptor antagonists inhibited those response.
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