2001 Fiscal Year Final Research Report Summary
High spatial-temporal resolution optical mapping of cardiac membrane action potentials using high intensity light emitting diodes and high spee3 digital video cameras
| Project/Area Number |
12680825
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAKUMA Ichiro Institute of Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo, Professor, 大学院・新領域創成科学研究科, 教授 (50178597)
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Itsuo Research Institute of Environmental Medicine, Nagoya University, Professor, 環境医学研究所, 教授 (30124720)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Optical Mapping of membrane action potential / Voltage sensitive dye / Arrhythmia / Spiral reentry / High intensity light emitting diode / high speed digital video camera |
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| Research Abstract |
We have developed a High spatial-temporal resolution optical mapping system of cardiac membrane action potentials using high intensity light emitting diodes and high-speed digital video cameras. A rabbit heart perfused in a Langendorff apparatus was stained with voltage sensitive dye : di-4-ANEPPS.Central wavelength of emitted fluorescence changes depending on membrane action potential. Thus by monitoring the emitted fluorescence from the perfused heart through optical long-pass filter, we can obtain membrane action potential information as change in fluorescence intensity at each point on the tissue. High intensity bluish green light emitting diodes were used as an excitation light source. Since it is a solid-state light source, it is easy to handle. Its intensity can be stabilized electronically. As a measurement device, We used high-speed digital video cameras that can capture images every 0.25 msec. We used it at frame rate of 1125 frames per second or 750 frames per second. As a r
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esults, high signal to noise ratio was obtained. Its spatial resolution was approximately O.2 mm. And its temporal resolution was 1 msec. Developed system can provide high resolution of measurement both for spatial resolution and temporal resolution while keeping relatively high signal to noise ratio (2.35 as an average) compared with conventional optical mapping system of cardiac action potentials. We also developed a software to process larga amount image data to obtain action potential information at any observation point on the heart tissue. In order to enlarge the observable area by the system, two high speed digital video cameras were used simultaneously. Compared with a single camera system, the double camera system could track the complicated excitation propagation pattern such as meandering of spiral wave and break up of the excitaion front. In conclusion, the developed high spatial-temporal resolution optical mapping system was effective in analyzing complicated electrical excitation pattern during arrhythmia. Less
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Research Products
(36 results)
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[Publications] Tatsuhiko Arahune, Naoki Tanoue, Etsuko Kobayashi, Ichiro Sakuma, Takeyoshi Dohi, Akira Misima, Hiroshi Inada, Nitaro Shibata, Mitsuru Yamamoto, Motoki Nihei, Haruo Honjo, Kaichiro Kamiya, Itsuo Kodama: "Computer Simulation of Reentrant Spiral Excitation Propagation in Cardiac Tissue"CJKMI2001. 56-58 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Akira Mishima, Tatsuhiko Arafune, Naoki Tanoue, Etsuko Kobayashi, Ichiro Sakuma, Nitaro Shibata, Haruo Honjo, Kaichiro Kamiya, Itsuo Kodama, Takeyoshi Dohi, Hiroshi Inada: "A high-resolition optical mapping and analysis system of action potentials during ventricular arrhythmias"CJKMI2001. 53-55 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] 田上直樹, 荒船龍彦, 三嶋晶, 小林英津子, 佐久間一郎, 稲田紘, 土肥健純, 仁瓶宗樹, 山本充, 丹羽良子, 本荘晴朗, 児玉逸雄, 柴田仁太郎: "2台の高速度デジタルビデオカメラレコーダを用いた広領域心臓活動電位光学マッピング"心電図. Volume21 No.5. 658 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] 三嶋晶, 荒船龍彦, 小林英津子, 佐久間一郎, 土肥健純, 稲田紘, 柴田仁太郎, 山本充, 丹羽良子, 本荘晴朗, 児玉逸雄: "心室スパイラルエントリのダイナミクス〜膜電位光学マッピングによる解析〜"医用電子と生体工学 第39巻特別号第40回日本ME学会. 大会論文集. 174 (2001)
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「研究成果報告書概要(和文)」より
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[Publications] Tatsuhiko Arahune, Naoki Tanoue, Etsuko Kobayashi, Ichiro Sakuma, Takeyoshi Dohi, Akira Misima, Hiroshi Inada, Nitaro Shibata, Mitsuru Yamamoto, Motoki Nihei, Haruo Honjo, Kaichiro Kamiya, Itsuo Kodama: CJKMI2001. 56. 58 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Akira Mishima, Tatsuhiko Arafune, Naoki Tanoue, Etsuko Kobayashi, Ichiro Sakuma, Nitaro Shibata, Haruo Honjo, Kaichiro Kamiya, Itsuo Kodama, Takeyoshi Dohi, Hiroshi Inada: "A high-resolition optical mapping and analysis system of action potentials during ventricular arrhythmias"Cjkmi2001. 53. 55 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] AKIRA Mishima, TATSUHIKO Arahume, ETSUKO Kobayashi, ICHlRO Sakuma, TAKEYOSHI Dohi, HlROSHI Inada, NITAEO Shibata, MITURU Yamamoto, HARUO Honjo, ITSUO Kodama.: "Dynamic of Ventricular Spiral Rentry"Japanese Journal of Medical Electronics and Biological Engineering. 39, suppl. 174 (2001)
Description
「研究成果報告書概要(欧文)」より
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