2002 Fiscal Year Final Research Report Summary
Development of cartilage proteoglycane as a new medical material
| Project/Area Number |
12793010
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Hirosaki University |
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Principal Investigator |
TAKAGAKI Keiichi Hirosaki University, School of Medicine, Professor, 医学部, 教授 (70163160)
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| Co-Investigator(Kenkyū-buntansha) |
KAKIZAKI Ikuko Hirosaki University, School of Medicine, Research Associate, 医学部, 助手 (80302024)
YOSHIHARA Syuichi Hirosaki University, Surgery School of Medicine, Lecture, 医学部, 講師 (00261454)
KATO Yoji Hirosaki University, Faculty of Education, Professor, 教育学部, 教授 (20194863)
IWAFUNE Mito Hirosaki University, School of Medicine, Research Associate, 医学部, 助手 (80312487)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Proteoglycan / Cartilase / Salmon |
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| Research Abstract |
(Function of Proteoglycan)
1. Malaria
I went to study to Penn. State University College of Medicine and did a joint research with Prof. Channe Gowda concerning the exact structure of the proteoglycan that inhibits the adhesion of Plasmodium falciparum infected red blood cells in human placenta. As a result, the chondroitin 4-sulfate chain of proteoglycan showed inhibitory activity.
2. Ulcerative colitis
We examined the effect of orally administered chondroitin sulfate proteoglycan(PG) against the dextran sodium sulfate(DSS) induced experimental colitis of rats. Colitis evaluations were made of bloody stool symptoms, hematological date, colon length and microscopic examination. PG improved the symptoms of bloody stools, increase of white blood cells, decrease of red blood cells, shortening of the colon length due to colitis and microscopically promoted healing of mucosal damage induced by DSS.
(Glycotechnological synthesis of proteoglycan)
1. Glycosaminoglycan chains
Reconstruction of Using a transglycosylation activity of testicular hyaluronidase, it was possible to custom synthesize glycosaminoglycan chains.
2. Attachment of GAG chains of protein
Using transglycosylation activity of endo-b-xylosidase, it was possible to transfer of the reconstructed GAG chains to the core protein.
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[Publications] Takagaki, K., Iwafune, M., Kakizaki, I., Ishido, K., Kato, Y., and Endo, M.: "Gleavage of the xylosyl serine linkage between a core peptide and a glycosaminoglycan chain by cellulases"J. Biol. Chem.. 277. 18397-18403 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishido, K., Takagaki, K., Iwafune, M., Yoshihara, S., Sasaki, M., and Endo, M.: "Enzymatic attachment of glycosaminoglycan chain to peptide using the sugar chain transfer reaction with endo-b-xylosidase"J. Biol. Chem.. 277. 11889-11895 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Takagaki, K., Munakata, H., Kakizaki, I., Iwafure, M.., Itabashi, T., and Endo, M.: "Domain struture of chondroitin sulfate E octasaccharides binding to type V collagen"J. Biol. Chem.. 277. 8882-8889 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Iwafune, M., Kakizaki, I., Yukawa, M., Kudo, D., Ots, S., Endo, M., and Takagaki, K.: "Reconstruction of glycosaminoglycan chains in decorin"Biochem. Biophys. Res. Commun.. 297. 1167-1170 (2002)
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[Publications] Kakizaki, I., Takagaki, K., Endo, Y., Kudo, D., Ikeya, H., Miyoshi, T., Baggenstoss, B.A., Tlapak-Simmons, V. L., Kumari, K., Nakane, A., Weigel, P. H., and Endo, M.: "Inhibition of hyalunonan synthesis in Streptococcus equi FM100 by 4-methylumbelliferone"Eur J Biochem.. 269. 5066-5075 (2002)
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「研究成果報告書概要(欧文)」より
