2001 Fiscal Year Final Research Report Summary
Puffer Culture Using Puffer Toxin-Immnopotentiation and Disease Prevention
| Project/Area Number |
12794005
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Nagasaki University |
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Principal Investigator |
NOGUCHI Tamao Faculty of Fisheries, Nagasaki University Professor, 水産学部, 教授 (40011910)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATANI Tomohiro Graduate School of Science and Technology, Nagasaki University Research Associate, 大学院・生産科学研究科, 助手 (90304972)
ARAKAWA Osamu Faculty of Fisheries, Nagasaki University Associate Professor, 水産学部, 助教授 (40232037)
TACHIBANA Katsuyasu Faculty of Fisheries, Nagasaki University Associate Professor, 水産学部, 助教授 (20171712)
TANIGAWA Akio Cooperative Association for Fish Processing Complex in Nagasaki Fishing Port. Executive Director/Chief Manufacturing Researcher, 専務理事/研究製造部長(研究者)
YAGI Motoaki Nagasaki Municipal Fisheries Center, Marine Culture Section, Chief Researcher, 養殖係, 係長(研究者)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Tiger puffer / Flsh cultule / Tetrodotoxin (TTX) / Immnopotentiation / Antibody balence / Blastogenesis / Net-cage / Fish scrap |
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| Research Abstract |
A total of 6 feeding experiments were conducted with cultured puffers of several age groups. The puffers were released in indoor tanks or net-cages in the sea, and reared for 30-60 days with 'commercial fish meal', 'fermented fish meal', 'TTX-containing diet' supplemented with crude toxins from the scraps of pear puffer, 'scrap meal' composed of crushed scraps of pear puffer and fish meal, etc. In each experiment, puffers were periodically examined to know their growth rate, lethal potency of each tissue and biochemical properties of the biood. At the end of experiment, their immunities were evaluated on the basis of antibody valance for sheep red blood cells and blastogenesis of splenocytes.
The muscles of all puffers tested were non-toxic in all the experiments, while the puffers fed with toxic diets accumulated small amounts of toxin (several MU/g) in the skin, and relatively large amounts (several tens MU/g) in the liver and ovary in most experiments. The toxin once accumulated has retained for a long time if the puffers fed with non-toxic diet successively. In respect of growth and biochemical properties of the blood, little difference was recognized between the test groups fed with toxic and non-toxic diets (hereafter designated as T and N, respectively). The immunities were considerably elevated by the administration of toxin ; antibody balance of T showed 1. 2-5-fold higher values than N in 5 experiments, and blastogenesis of T showed 1. 1-2. 5-fold higher values than N in 2 experiments. Furthermare, survival rate (64-70%) of T was 10-15% higher than N (around 54%) in an outdoor experiment in the summer season.
From these results, it can be concluded that the use of puffer toxin-containing diets could be very effective for growing up healthy puffers in culture practice.
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