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2002 Fiscal Year Final Research Report Summary

Comprehensive isolation and functional analysis of the genes whose expressions are specifically induced upon entry into the Go phase of cell cycle

Research Project

Project/Area Number 12794010
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionOsaka University

Principal Investigator

NOJIMA Hiroshi  RIMD, Professor, 微生物病研究所, 教授 (30156195)

Co-Investigator(Kenkyū-buntansha) TAMAI Katuyuki  Cyclex, Co. Ltd., President, 部長(研究職)
OKUZAKI Daisuke  RIMD, Research Assistant, 微生物病研究所, 助手 (00346131)
YABUTA Norikazu  RIMD, Research Assistant, 微生物病研究所, 助手 (10343245)
TOJI Shingo  MBL Co. Ltd., Research Fellow, 研究員
Project Period (FY) 2000 – 2002
Keywordsquiescent state / growth inhibitory factor / primary cell / transmembrane motif / serum starvation / contact inhibition / cancer cell line / LOH
Research Abstract

Mammalian cells stops growth and enter into the static phase or G_0 phase when the concentration of fetal calf serum in the culture medium was reduced from 10% to 0.5%. It was believed that a certain proliferation inhibitory factors exist in the medium harboring the cells in the G_0 phase to maintain the static condition. However, very few studies have been reported on the genes and their gene products that define the G_0 phase. Thus, if a large scale isolation of the genes whose expression was induced is performed, it would be possible to isolate the gene that is required to maintain the static phase by growth-inhibitory function. To isolate such genes comprehensively, we used an improved cDNA library subtraction p as we described previously. Using this method, we have so far been able to isolate more than 60 genes whose expression is induced in growth arrest condition and termed the transcript induced in growth arrest, TIGA, genes. Transcription of some of the TIGA genes are also induced upon contact inhibition of the cell growth. One of the TIGA genes, TIGA1, encodes a small protein with a single transmembrane motif. Ecopically expressed Tiga1 potently inhibited cell growth. Functional analyses of these TIGA genes might shed light to understanding the molecular mechanisms of induction and maintenance of G_0 state of the cell cycle.

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Kataoka, T.R., Ito, A., Asada, H., Watabe, K., Nishiyama, K., Nakamoto, K., Itami, S., Yoshikawa, K., Ito, M., Nojima, H., Kitamura, Y: "Annexin VII as a novel prognostic marker of malignant melanoma"J. J. Cancer Res.. 91・2. 75-83 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito, A., Kataoka, T.R., Watanabe, M., Nishiyama, K., Mazaki, Y, Sabe, H., Kitamura, Y., Nojima, H.: "A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation"EMBO J.. 19・4. 562-571 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito, A., Katoh, F., Kataoka, T.R., Okada, M., Tsubota, N., Asada, H., Yoshikawa, K., Maeda, S., Kitamura, Y., Yamasaki, H., Nojima, H.: "A role for heterologous gap junctions between melanoma and endothelial cells in metastasis"J. Clinc. Invest.. 105・9. 1189-1197 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watabe, K., Nakamoto, K., Ito, A., Okada, M., Tsubota, N., Endo, Y., Shinomura, Y., Matsuzawa, Y, Nojima, H.: "Structure, expression and chromosome mapping of MLZE, a novel candidate marker for squamous cell lung carcinoma"Japanese Journal of Cancer Research. 92・2. 140-151 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, S.H., Ikawa, M., Ito, A., Okabe, M., Nojima, H.: "Cyclin G1 is involved in G2/M arrest in response to DNA damage and growth control after damage recovery"Oncogene. 20・10. 3290-3300 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamoto, K., Ito, A., Watabe, K., Koma, Y., Asada, H., Yoshikawa, K., Shinomura, Y., Matsuzawa, Y., Nojima, H., Kitamura, Y.: "Increased Expression of a Nucleolar Nop5/Sik Family Member in Metastatic Melanoma Cells. Evidence for its Role in Nucleolar Sizing and Function"Journal of American Pathology. 159・4. 1363-1374 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshisue, H.Suzuki, K.Kawabata, A., Ohya, T., Zhao, H., Sakurada, K., Taba, Y, Sasaguri, T., Sakai, N., Yamashita, S., Matsuzawa, Y., Nojima, H.: "Large scale isolation of non-uniform shear stress-responsive genes from cultured human endothelial cells through the preparation of a subtracted cDNA library"Atherosclerosis. 162・2. 323-334 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujii, T., Tamura, K., Masai, K., Tanaka, H., Nishimune, Y., Nojima, H.: "Use of stepwise subtraction to comprehensively isolate mouse genes whose transcription is upregulated during spermiogenesis"EMBO Report. 3・4. 367-372 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshioka, N., Fuji, S., Shimakage, M., Kodama, K., Hakura, A., Yutsudo, M., Inoue, H., Nojima, H: "Suppression of anchorage-independent growth of human cancer cell lines by the TRIF52/periostin/OSF-2 gene"Experimental Cellular Research. 279・1. 91-99 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, S.H., Nojima, H.: "Cyclin G1 mediates the association of MDM2 with ARE and promotes p53 accumulation"Genes Cells. 7・8. 869-880 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito, A., Koma, Y., Watabe, K., Nagano, T., Endo, Y, Nojima, H., Kitamura, Y.: "A truncated isoform of the PP2A B56gamma regulatory subunit may promote genetic instability and cause tumor progression"Am. J. Pathol.. 162・1. 81-91 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito, A., Koma, Y, Sohda, M., Watabe, K., Nagano, T., Misumi, Y., Nojima, H., Kitamura, Y.: "Localization of the PP2A B56gamma regulatory subunit to the Golgi complex : possible role in vesicle transport and migration"Am. J. Pathol.. 162・2. 479-489 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito, A., Jippo, T., Wakayama, T., Morii, E., Koma, Y.I., Onda, H., Nojima, H., Iseki, S., Kitamura, Y.: "SgIGSF : a new mast-cell adhesion molecule used for attachment to fibroblasts and transcriptionally regulated by MITF"Blood. 101・4. 2601-2608 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kataoka, T.R., Ito, A., Asada, H., Watabe, K., Nishiyama, K., Nakamoto, K., Itami, S., Yoshikawa, K., Ito, M., Nojima, H., and Kitamura, Y.: "Annexin VII as a novel prognostic marker of malignant melanoma"J. J. Cancer Res.. 91. 75-83 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, A., Kataoka, T.R., Watanabe, M., Nishiyama, K., Mazaki, Y., Sabe, H., Kitamura, Y. and Nojima, H.: "A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation"EMBO J.. 19. 562-571 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, A., Katoh, F., Kataoka, T.R., Okada, M., Tsubota, N., Asada, H., Yoshikawa, K., Maeda, S., Kitamura, Y., Yamasaki, H.. and Nojima, H..: "A role for heterologous gap junctions between melanoma and endothelial cells in metastasis"J. Clinc. Invest.. 105. 1189-1197 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watabe, K., Nakamoto, K., Ito, A., Okada, M., Tsubota, N., Endo, Y., Shinomura, Y., Matsuzawa, Y., Nojima, H.: "Structure, expression and chromosome mapping of MLZE, a novel candidate marker for squamous cell lung carcinoma"J. J. Cancer Res.. 92. 140-151 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, S.H, Ikawa, M., Ito, A., Okabe, M., Nojima, H.: "Cyclin G1 is involved in G2/M arrest in response to DNA damage and growth control after damage recovery"cogene. 20. 3290-3300 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamoto, K., Ito, A., Watabe, K., Koma, Y., Asada, H., Yoshikawa, K., Shinomura, Y., Matsuzawa, Y., Nojima, H. and Kitamura, Y.: "Increased Expression of a Nucleolar Nop5/Sik Family Member in Metastatic Melanoma Cells Evidence for its Role m Nucleolar Sizing and Function"J. Am. Pathol.. 159. 1363-1374 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshisue, H., Suzuki, K., Kawabata, A., Ohya, T., Zhao, H., Sakurada, K., Taba, Y., Sasaguri, T., Sakai, N., Yamashita, S., Matsuzawa, Y. and Nojima, H.: "Large scale isolation of non-uniform shear stress-responsive genes from cultured human endothelial cells through the preparation of a subtracted cDNA library"Atherosclerosis. 162(2). 323-334 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujii, T., Tamura, K., Masai, K., Tanaka, H., Nishimune, Y. and Nojima, H.: "Use of stepwise subtraction to comprehensively isolate mouse genes whose transcription is upregulated during spermiogenesis"EMBO Rep.. 3(4). 367-372 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshioka,N., Fuji, S., Shimakage, M., Kodama, K., Hakura, A., Yutsudo, M., Inoue, H. and Nojima, H.: "Suppression of anchorage-independent growth of human cancer cell lines by the TRIF52/periostin/OSF-2 gene"Exp. Cell Res.. 279. 91-99 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura, S.H. and Nojima, H.: "Cyclin G1 mediates the association of MDM2 with ARF and promotes p53 accumulation"Genes Cells. 7(8). 869-880 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, A., Koma, Y., Watabe, K., Nagano, T., Endo, Y., Nojima, H. and Kitamura, Y.: "A truncated isoform of the PP2A B56gamma regulatory subunit may promote genetic instability and cause tumor progression"Am. J. Pathol.. 162(1). 81-91 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, A., Koma, Y., Sohda, M., Watabe, K., Nagano, T., Misumi, Y., Nojima, H. and Kitamura, Y.: "Localization of the PP2A B56gamma regulatory subunit to the Golgi complex : possible role in vesicle transport and migration"Am. J. Pathol.. 162(2). 479-489 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ito, A., Jippo, T., Wakayama, T., Morii, E., Koma, Y.I, Onda, H., Nojima, H., Iseki, S. and Kitamura, Y.: "SgIGSF : a new mast-cell adhesion molecule used for attachment to fibroblasts and transcriptionally regulated by MITF"Blood. 101(4). 2601-2608 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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