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2001 Fiscal Year Final Research Report Summary

In vivo gene therapy utilising transferrin receptor-operated transfer

Research Project

Project/Area Number 12835004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SHICHIRI Masayoshi  Tokyo Medical and Dental University, Department of Clinical and Molecular Endocrinology, Lecturer, 医学部・附属病院, 講師 (10206097)

Project Period (FY) 2000 – 2001
Keywordsgene therapy / LDL receptor deficiency / balloon-injured rat / transferrin receptor / max gene / Light Cycler
Research Abstract

Cationic liposome DNA complex (CLDC) intravenous gene delivery has been shown to direct gene expression to vascular endothelial cells, monocytes, and macrophages. CLDC gene delivery avoids immune system reactions associated with viral vectors, yet the low efficiency of gene delivery using CLDC has limited the use of non-viral vectors for gene therapy. Ligand-facilitated transfer of CLDC has been shown to markedly increase in vitro gene delivery efficiency, but has not increased in vivo efficiency to the magnitude of adenoviral vectors and has remained unsuccessful in applications to systemic gene therapy. Introduction of ligands such as transferrin and asialofetuin into a CLDC has been reported to generate in vivo expression of reporter plasmids while others report low transfer efficiency. We have demonstrated that a combination of CLDC-transferrin enhanced intravenous delivery in circulating leukocytes. Transferrin-facilitated intravenous delivery of cationic liposome CDNA complexes could serve as an important adjunct therapy for the treatment of disorders, especially those in which deficiency of a single gene can be replaced by transfer of an exogenous gene into circulating cells.

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Shichiri M, Yokokura M, Marumo F, Hirata Y: "Endothelin-1 inhibits apoptosis of vascular smooth muscle cells induced by nitric oxide and serum deprivation via MAP kinase pathway"Arteriosclerosis Throm Vasc Biol. 20. 989-997 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohta K, Shichiri M, Kameya T, Matsubara O, Imai T, Marumo F, Hirata Y: "Thymic hyperplasia as a source of ectopic ACTH production"Endocrine Journal. 47. 487-492 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Doi M, Shichiri M, Yoshida Y, Marumo F, Hirata Y: "Suppression of integrin αV expression by endothelin-1 in vascular smooth muscle cells"Hypertension Research. 23. 643-649 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Doi M, Shichiri M, Katsuyama K, Marumo F, Hirata Y: "Cytokine-activated p42/p44 MAP kinase is involved in inducible nitric oxide synthase gene expression independent from NF-κB activation in vascular smooth muscle cells"Hypertension Research. 23. 659-667 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaki H, Shichiri M, Marumo F, Hirata Y: "Adrenomedullin stimulates proline-rich tyrosine kinase 2 in vascular smooth muscle cells"Endocrinology. 142. 564-572 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiigai T, Shichiri M: "Late escape from the anti-proteinuric effect of ACE inhibitors in non-diabetic renal disease"American Journal of Kidney Diseases. 37. 477-483 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishimaru S, Shichiri M, Mineshita S, Hirata Y: "Role of endothelin-1/endothelin receptor system in endotoxic shock rats"Hypertension Research. 24. 119-126 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shichiri M, Hirata Y: "Anti-angiogenesis signals by endostatin"FASEB Journal. 15. 1044-1053 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaki H, Sato R, Shichiri M, Hirata Y: "A patient with type 1 diabetes mellitus and cerebellar ataxia associated with high titer of circulating anti-glutamic acid decarboxylase antibodies"Endocrine Journal. 48. 261-268 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsushita M, Shichiri M, Imai T, Iwashina M, et al.: "Co-expression of urotensin II and its receptor (GPR14) in human cardiovascular and renal tissues"Journal of Hypertension. 19. 2185-2190 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Doi M, Shichiri M, Katsuyama K, Ishimaru S, Hirata Y: "Cytokane-activated Jak-2 is involved in inducible nitric oxide synthase expression independent from NF-κB activation in vascular smooth muscle cells"Atherosclerosis. 160. 123-132 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shichiri M, Yoshinaga K, Hisatomi H, Sugihara K, Hirata Y: "Genetic and epigenetic inactivation of mitotic checkpoint genes hBUB1 and hBUBR1 and their relationship to survival"Cancer Research. 62. 13-17 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shichiri M, Yokokura M, Marumo F, Hirata Y: "Endothelin-1 inhibits apoptosis of vascular smooth muscle cells induced by nitric oxide and serum deprivation via MAP kinase pathway"Arteriosclerosis Throm Vasc Biol. 20. 989-997 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohta K, Shichiri M, Kameya T, Matsubara O, Imai T, Marumo F, Hirata Y: "Thymic hyperplasia as a source of ectopic ACTH production"Endocrine. 47. 487-492 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Doi M, Shichiri M, Yoshida Y, Marumo F, Hirata Y: "Suppression of integrin av expression by endothelin-1 in vascular smooth muscle cells"Hypertension Res. 23. 643-649 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Doi M, Shichiri M, Katsuyama K, Marumo F, Hirata Y: "Cytokine-activated p42/p44 MAP kinase is involved in inducible nitric oxide synthase gene expression independent from NF-kB activation in vascular smooth muscle cells"Hypertension Res. 23. 659-667 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaki H, Shichiri M, Marumo T, Hirata Y: "Adrenomedullin stimulates proline-rich tyrosine kinase 2 in vascular smooth muscle cells"Endocrinology. 142 : 564. 572 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiigai T, Shichiri M: "Late escape from the anti-proteinuric effect of ACE inhibitors in non-diabetic renal disease"Am J Kidney Dis. 37. 477-483 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishimaru S, Shichiri M, Mineshita S, Hirata Y: "Role of endothelin-1 /endothelin receptor system in endotoxic shock rats"Hypertension Res. 24. 119-126 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shichiri M, Hirata Y: "Anti-angtogenesis signals by endostatin"FASEB J. 15. 1044-1053 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaki H, Sato R, Shichiri M, Hirata Y: "A. patient with type 1 diabetes mellitus and cerebellar ataxia associated with high titer of circulating anti-glutamic acid decarboxylase antibodies"Endocrine J. 48. 261-268 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M, Shichiri M, Imai T, Iwashina M, Tanaka H, Takasu N, Hirata Y: "Co-expression of urotensin II and its receptor (GPR14) in human cardiovascular and renal tissues"J Hypertens. 19. 2185-2190 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] . Doi M, Shichiri M, Katsuyama K, Ishimaru S, Hirata Y: "Cytokine activated Jak-2 is involved in inducible nitric oxide synthase expression independent from NF-kB activation in vascular smooth muscle cells"Atherosclerosis. 160. 123-132 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shichiri M, Yoshinaga K, Hisatomi H, Sugihara K, Hirata Y: "Genetic and epigenetic inactivation of mitotic checkpoint genes hBUB1 and hBUBR1 and their relationship to survival"Cancer Res. 62. 13-17 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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