2003 Fiscal Year Final Research Report Summary
Design of artificial proteins using hydrophobic interactions
| Project/Area Number |
13031023
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
KOBATAKE Eiry Tokyo Tech., Dept. Biological Information., Associate Professor, 大学院・生命理工学研究科, 助教授 (00225484)
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| Project Period (FY) |
2001 – 2003
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| Keywords | hydrophobic interaction / artificial protein / cell adhesion / cell proliferation / elastin / RGD / extracellular matrix / EGF |
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| Research Abstract |
The development of techniques for immobilizing proteins with retaining their functions even on a solid surface is important. However, it is difficult to control the orhentation of protein on a solid-surface. To overcome this disadvantage, we have proposed a novel technique for assembling functional proteins. It is based on a simple hydrophobic intetaction between protein and solid-surface. In this method, a target protein is fused with a hydrophobic peptide unit, which has an ability to assemble on a hydropbobic solid-surface. In this condition, a relatively hydrophilic target protein part in the fusion protein sbould be oriented to the opposite side of solid-surface. Therefore, it is expected to realize a simple and efficient method for assembling functional proteins. We have designed novel proteins consisting of hydrophobic peptide sequence as a self-assembled structural unit and molecular recognition peptide as a functional unit. The resulting proteins retained their functions on a solid-phase surface and they were applied to biocensing and cellular engineering.
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