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2005 Fiscal Year Final Research Report Summary

Regulation of initiation and elongation stages of DNA replication

Research Project

Project/Area Number 13043029
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka University

Principal Investigator

TAKISAWA Haruhiko  Osaka University, Graduate School of Science, Department of Biological Sciences, Professor, 大学院理学研究科, 教授 (60154944)

Co-Investigator(Kenkyū-buntansha) SHIRAHIGE Katsuhiko  Tokyo Institute of Technology, Gene Research Center, Associate Professor, バイオ研究基盤支援総合センター, 助教授 (90273854)
Project Period (FY) 2001 – 2005
KeywordsDNA replication / Initiation complex / GINS / rep liso me / ChIP-chip method / Replication checkpoint
Research Abstract

UsingXenopuseggextracts and budding yeast,we investigatedtheregulationof initiation andelongation stages of DNA replication. We have clarified conserved and diverged regulation mechanisms for DNA replication.
We have identified a novel and highly conserved replication protein complex, GINS, as an essential component required for the assembly of initiation machinery of DNA replication. We have also identified conserved components of replisomes as GINS, Cdc45, Tofl/Timl, Csm3/Tipin and Mrcl/claspin. We have also identified Xenopus homolog of yeast Cut5/Dpb 11 and Sld2/Drc 1 as Xenopus CutS and RecQ4. These proteins show diverged structure and function compared with yeast proteins, suggesting that these proteins gain some additional functions during evolution of metazoans.
We developed a high-density oligonucleotide array that was able to analyze yeast chromosome VI at a 300-base pair (bp) resolution and introduced ChIP-chip (Chromatin immuno-precipitation combined with DNA chip) as a very powerful tool to visualize the dynamic changes of replication proteins and progression of DNA replication. This technology opens up the way to understand how local protein-protein or protein-DNA interactions lead to the dynamic changes of chromosome structure and clarified 1) how DNA replication and cell cycle checkpoint are coordinated, and 2) how DNA transcription is coordinated with sister chromatid cohesion.

  • Research Products

    (39 results)

All 2006 2005 2004 2003

All Journal Article (36 results) Book (3 results)

  • [Journal Article] Regulation of Replication Licensing by Acetyltransferase Hbol.2006

    • Author(s)
      Iizuka, M.
    • Journal Title

      Mol. Cell. Biol. 26

      Pages: 1098-1108

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Localization of MCM2-7, Cdc45, and GINS to the Site of DNA Unwinding during Eukaryotic DNA2006

    • Author(s)
      Pacek, M.
    • Journal Title

      Mol. Cell 21

      Pages: 581-587

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The N-terminal noncatalytic region of Xenopus RecQ4 is required for chromatin binding of DNA polymerase a in the initiation of DNA replication2006

    • Author(s)
      Matsuno, K.
    • Journal Title

      Mol. Cell. Biol. 26

      Pages: 4843-4852

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The phosphorylated C-terminal domain of Xenopus Cut5 directly mediates ATR-dependent activation of Chk12006

    • Author(s)
      Hashimoto, Y.
    • Journal Title

      Genes Cells 11

      Pages: 993-1007

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Protein Phosphatase 2A protects centromeric sister chromatid cohesion.2006

    • Author(s)
      Riedel, C.G.
    • Journal Title

      Nature 441

      Pages: 53-61

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Chromosomal Association of the Smc5/6 Complex Reveals that It Functions in Differently Regulated Pathways.2006

    • Author(s)
      Betts, H.
    • Journal Title

      Mol. Cell 23

      Pages: 755-767

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulation of Replication Licensing by Acetyltransferase Hbol.2006

    • Author(s)
      Iizuka, M., Matsui, T., Takisawa, H., Smith, M.M.
    • Journal Title

      Mol. Cell. Biol. 26(3)

      Pages: 1098-1108

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Localization of MCM2-7, Cdc45, and GINS to the Site of DNA Unwinding during Eukaryotic DNA.2006

    • Author(s)
      Pacek, M., Tutter, A.V., Kubota, Y., Takisawa, H., Walter, J.C.
    • Journal Title

      Mol. Cell 21(4)

      Pages: 581-587

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The N-terminal noncatalytic region of Xenopus RecQ4 is required for chromatin binding of DNA polymerase a in the initiation of DNA replication.2006

    • Author(s)
      Matsuno, K., Kumano, M., Kubota, Y., Hashimoto, Y., Takisawa, H.
    • Journal Title

      Mol. Cell. Biol. 26(13)

      Pages: 4843-4852

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The phosphorylated C-terminal domain of Xenopus Cut5 directly mediates ATR-dependent activation of Chkl2006

    • Author(s)
      Hashimoto, Y., Tsujimura, T.Sugino, A., Takisawa, H.
    • Journal Title

      Genes Cells 11(9)

      Pages: 993-1007

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Protein Phosphatase 2A protects centromeric sister chromatid cohesion.2006

    • Author(s)
      C.G.Riedel, V.L.Katis, Y.Katou, S.Mori, W.Helmhart, M.Galova, M.Petronczki, J.Gregan, B.Cetin, I.Mudrak, E.Ogris, K.Mechtler, L.Pelletier, F.Buchholz, K.Shirahige, K.Nasmyth.
    • Journal Title

      Nature 441(7089)

      Pages: 53-61

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Chromosomal Association of the Smc5/6 Complex Reveals that It Functions in Differently Regulated Pathways.2006

    • Author(s)
      H.Betts Lindroos, L.Strom, T.Itoh, Y.Katou, K.Shirahige*, C.Sjogren
    • Journal Title

      Mol Cell. 22(6)

      Pages: 755-767

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Intrinsic nuclear import activity of geminin is essential to prevent re-initiation of DNA replication in Xenopus eggs.2005

    • Author(s)
      Yoshida, K
    • Journal Title

      Genes Cells 10

      Pages: 63-73

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae2005

    • Author(s)
      Takami, H.
    • Journal Title

      Genes Cells 10

      Pages: 193-206

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A checkpoint control linking meiotic S phase and recombination initiation in fission yeast.2005

    • Author(s)
      Tonami, Y.
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A 102

      Pages: 5797-5801

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification and characterization of rns4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae : Evidence for altered ambient response resulting in transportation of the secretory protein to vacuoles.2005

    • Author(s)
      Unno, K.
    • Journal Title

      FEMS Yeast Res 5

      Pages: 801-812

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intrinsic nuclear import activity of geminin is essential to prevent re-initiation of DNA replication in Xenopus eggs.2005

    • Author(s)
      Yoshida, K., Takisawa, H., Kubota, Y.
    • Journal Title

      Genes Cells 10(1)

      Pages: 63-73

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae.2005

    • Author(s)
      H.Tamaki, C.W.Yun, T.Mizutani, T.Tsuzuki, Y.Takagi, M.Shinozaki, Y.Kodama, K.Shirahige, H.Kumagai.
    • Journal Title

      Genes Cells 10(3)

      Pages: 193-206

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A checkpoint control linking meiotic S phase and recombination initiation in fission yeast.2005

    • Author(s)
      Y.Tonami, H.Murakami, K.Shirahige, M.Nakanishi.
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A. 102(16)

      Pages: 5797-801

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Kitamoto Identification and characterization of ms4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae : Evidence for altered ambient response resulting in transportation of the secretory protein to vacuoles.2005

    • Author(s)
      K.Unno, P.R.Juvvadi, H.Nakajima, K.Shirahige, K.
    • Journal Title

      FEMS Yeast Res. 5(9)

      Pages: 801-812

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Reciprocal association of the budding yeast ATM-related proteins Tell and Mecl with telomeres in vivo2004

    • Author(s)
      Takata, H.
    • Journal Title

      Mol. Cell 14

      Pages: 515-522

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cohesin relocation from sites of chromosomal loading to places of convergent transcriptions.2004

    • Author(s)
      Lengronne, A.
    • Journal Title

      Nature 430

      Pages: 573-578

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Yeast Pho85 kinase is required for proper gene expression during the diauxic shift.2004

    • Author(s)
      Nishizawa, M.
    • Journal Title

      Yeast 21

      Pages: 903-918

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Postreplicative recruitment of Cohesion to double-strand breaks is required for DNA repair.2004

    • Author(s)
      Strom, L.
    • Journal Title

      Mol. Cell 16

      Pages: 1003-1015

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Spo13 Facilitates Monopolin Recruitment to Kinetochores and Regulates Maintenance of Centromeric Cohesion during Yeast Meiosis.2004

    • Author(s)
      Katis, V.L.
    • Journal Title

      Curr. Biol. 14

      Pages: 2183-2196

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reciprocal association of the budding yeast ATM-related proteins Tell and Mecl with telomeres in vivo.2004

    • Author(s)
      Takata, H.
    • Journal Title

      Mol. Cell 14(4)

      Pages: 515-522

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cohesin relocation from sites of chromosomal loading to places of convergent transcriptions.2004

    • Author(s)
      A.Lengronne, Y.Katou, S.Mori, S.Yokobayashi, G.Kelly, T.Itoh, Y.Watanabe, K.Shirahige*, F.Uhlmann*
    • Journal Title

      Nature 430(6999)

      Pages: 573-578

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Yeast Pho85 kinase is required for proper gene expression during the diauxic shift.2004

    • Author(s)
      M.Nishizawa, Y.Katou, K.Shirahige, A.Toh-E
    • Journal Title

      Yeast 21(11)

      Pages: 903-918

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Sjogren Postreplicative recruitment of Cohesin to double-strand breaks is required for DNA repair.2004

    • Author(s)
      L.Strom, H.B.Lindroos, K.Shirahige, C.
    • Journal Title

      Mol. Cell 16(6)

      Pages: 1003-1015

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Spol3 Facilitates Monopolin Recruitment to Kinetochores and Regulates Maintenance of Centromeric Cohesion during Yeast Meiosis.2004

    • Author(s)
      V.L.Katis, J.Matos, S.Mori, K.Shirahige, W.Zachariae, K.Nasmyth
    • Journal Title

      Curr Biol. 14(24)

      Pages: 2183-2196

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] CDK- and Cdc45-dependent priming of the MCM complex on chromatin during S-phase in Xenopus egg extracts.2003

    • Author(s)
      Masuda, T
    • Journal Title

      Genes Cells 8

      Pages: 145-161

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication.2003

    • Author(s)
      Kubota, Y.
    • Journal Title

      Genes & Dev. 17

      Pages: 1141-1152

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Xenopus Cut5 is essential a CDK-dependent process in the initiation of DNA replication2003

    • Author(s)
      Hashimoto, Y
    • Journal Title

      EMBO J. 22

      Pages: 2526-2535

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] CDK-and Cdc45-dependent priming of the MCM complex on chromatin during S-phase in Xenopus egg extracts.2003

    • Author(s)
      Masuda, T., Mimura, S., Takisawa, H.
    • Journal Title

      Genes Cells 8(2)

      Pages: 145-161

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication.2003

    • Author(s)
      Kubota' Y., Takase' Y., Komori, Y., Hashimoto, Y., Arata, T., Kamimura, Y., Araki, H., Takisawa, H.
    • Journal Title

      Genes & Dev. 17(9)

      Pages: 1141-1152

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Xenopus Cut5 is essential for a CDK-dependent process in the initiation of DNA replication.2003

    • Author(s)
      Hashimoto, Y., Takisawa, H.
    • Journal Title

      EMBOJ. 22(10)

      Pages: 2526-2535

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Methods in Enzymology "DNA Repair" (edited by J. Campbell)2006

    • Author(s)
      Katou, Y.
    • Total Pages
      575
    • Publisher
      Elsevier Life Sciences (CA)
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] DNA複製・修復がわかる(分担 DNA複製のライセンス化)2004

    • Author(s)
      久保田弓子
    • Total Pages
      133
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 細胞核のダイナミックス(分担 再構成核におけるDNA複製の解析)2004

    • Author(s)
      久保田弓子
    • Total Pages
      206
    • Publisher
      シュプリンガー・フェアラーク東京
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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