2005 Fiscal Year Final Research Report Summary
Death and regeneration of genome Biology of DNAdouble-strand break and the response
| Project/Area Number |
13141201
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOBAYAHSI Ichizo The University of Tokyo, Department of Medical Genome Sciences, Graduate School of Frontier Science, Professor, 大学院新領域創成科学研究科, 教授 (30126057)
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| Co-Investigator(Kenkyū-buntansha) |
HANDA Naofumi Department of Medical Genome Sciences, Graduate School of Frontier Science, SA Research Associates, 大学院新領域創成科学研究科, 産学官連携研究員(特任助手) (00396855)
ITAYA Mitsuyasu Mitsubishi Kagaku Institute of Life Sciences, Senior Research Scientist, 主任研究員 (60374013)
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| Project Period (FY) |
2001 – 2005
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| Keywords | Genome / Bacteria / Genome rearrangements / Genome evolution / Comparative genomics / Protein structure / Biotechnology / Environment |
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| Research Abstract |
We found multiplication and transposition of a restriction-modification gene complex. We measured intracellular molecules of E4aRI and MEcoRI and found that they are not very different in stability. We found that solitary methyltransferase protects genome from attack by a restriction-modification gene complex. We measured broken linear chromosomes in various recombinationTelated mutants of E. coli. We ' analyzed illegitimate recombination dependent on restriction and homology. We found that phage-mediated homologous recombination protects phage genome from attack by be DI restrictioninoclification systems.
We obtained restriction enzyme PabI from Pyrococcus abyssi and found that it generates TAX, a novel type of restriction terminus. We obtained and characterized MPabI.
We tried somatic gene targeting with mutation-reporter mouse with adenovirus vector and with DNA/RNAchimera.
We compared closely-related bacterial genomes to reconstruct genome rearrangements. With Staphylococcus aureus, we listed middle-sized polymorphisms. We found recombination in the two specificity domains in S subunit of Type I restriction enzyme. We reconstructed history of paralog clusters. With Neiseria, we found that a filamentous phage integrates into chromosome with its own transposase. This transposase caused various genome rearrangements. We explained an apparently complex polynicaphisnis by few steps of action of mobile elements.
We modeled branch migration of a Holliday structure as an asymmetric random walk. We developed a random walk model for interference in meiotic recombination. We analyzed conditions for evolution of addiction genes. Space structure was found to be impcatant.
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