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2005 Fiscal Year Final Research Report Summary

Proton pump ATPase as nano motors

Research Project

Project/Area Number 13142204
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionMicrobial Chemistry Research Center (2004-2005)
Osaka University (2001-2003)

Principal Investigator

FUTAI Masamistu  Microbial Chemistry Research Center, Futai Special Laboratory, Special Researcher, 微生物化学研究センター・二井特別研究室, 特別研究員 (50012646)

Co-Investigator(Kenkyū-buntansha) SUN-WADA Ge-hong  Doshisya Womens' College pharmaceutical, Assistant Professor, 薬学部, 助教授 (00314427)
TAKAZAWA Shinn  Tohoku University, Graduate School of Medicine, Assistant Professor, 医科学系研究科, 助教授 (50187944)
Project Period (FY) 2001 – 2005
KeywordsATP / F-TPase / V-ATPase / Lisosom / nano machine / nano motors
Research Abstract

Three classes of proton pumping ATPase are known : P-TPase, F-TPase and V-ATPase. F-ATPase (ATP synthase) and V-ATP(prothn pump of endomembrance organelles such as lysosome and endosome) are closely similar in subunit structure and mechanism. They couples proton transport and ATPase chemistry through subunit rotation. Our extensive research results are showing:(1) Purified and membrane-bound F-ATPases are rotating during ATP hydrolysis. Their rotations are stochastic, and driven by hinge domain of the β subunit. (2) V-ATPases are also rotating proton pump, and their diverse isoforms specific for unique cells were found. (3) V-ATPase has domains required for specific localization and possibly for sensing organeller luminal pH. These results have been widely recognized in the field biochemistry and cell biology.

  • Research Products

    (68 results)

All 2006 2005 2004 2003 2002 2001 Other

All Journal Article (64 results) Book (4 results)

  • [Journal Article] High-speed rotation of Escherichia coli ATP synthase F_1 sector : ε subunit-sensitive rotation2006

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 281

      Pages: 4126-4131

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] V-ATPase interact with ARNO and Arf6 in early endosomes and regulates the protein degradative pathway2006

    • Author(s)
      M, Futai.
    • Journal Title

      Nature Cell Biol. 8

      Pages: 124-136

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Selective assembly of subunit isoforms in mouse kidney.2006

    • Author(s)
      K, Son.
    • Journal Title

      J.Bioenerg.Biomemb.(2006) (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Distinct expression patterns of different subunit isoforms of the V-ATPase in the rat epididymis2006

    • Author(s)
      M, Futai.
    • Journal Title

      Biol.Reprod. 74

      Pages: 185-194

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stochastic proton pumping ATPases : from single molecules to Diverse physiological roles.2006

    • Author(s)
      M, Futai.
    • Journal Title

      UBMB Life (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] High-speed rotation of Escherichia coli ATP synthase F1 sector : s subunit-sensitive rotation2006

    • Author(s)
      Masamistu Futai., Mayumi Nakanishi
    • Journal Title

      J. Biol. Chem. 281

      Pages: 4126-4131

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] V-ATPase interact with ARNO and Arf6 in early endosomes and regulates the protein degradative pathway2006

    • Author(s)
      Masamistu Futai
    • Journal Title

      Nature Cell Bio1. 8

      Pages: 124-136

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Selective assembly of subunit isoforms in mouse kidney.2006

    • Author(s)
      Ge-Hong., Sun-Wada
    • Journal Title

      J. Bioenerg. Biomemb. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Distinct expression patterns of different subunit isoforms of the V-ATPase in the rat epididymis2006

    • Author(s)
      Masamistu Futai
    • Journal Title

      Biol. Reprod. 74

      Pages: 185-194

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Stochastic proton pumping ATPases : from single molecules to diverse physiological roles.2006

    • Author(s)
      Masamistu Futai
    • Journal Title

      UBMB Life. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Simple and straight forward construction of a mouse gene targeting vector using in vitro transposition reaction2005

    • Author(s)
      M, Futai.
    • Journal Title

      Nucleic Acid Res. 33, No5

      Pages: e52 (1-8)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ATP-dependent rotation of mutant ATP synthases lacking proton transport2005

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 28

      Pages: 23797-23801

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Simple and straight forward construction of a mouse gene targeting vector using in vitro transposition reaction2005

    • Author(s)
      Masamistu Futai., Ge-Hong Sun-Wada
    • Journal Title

      Nucleic Acid Res. 33,No5

      Pages: e52(1-8)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ATP-dependent rotation of mutant ATP synthases lacking proton transport2005

    • Author(s)
      Masamistu Futai
    • Journal Title

      J. Biol. Chem. 280

      Pages: 23797-23801

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mouse proton pump ATPase C subunit Isoforms (C2-a and C2-b) sp ecifically expressed in kidney and lung.2004

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 44843-44851

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulatory elements, directing gut-specific expression of GATA6 gene during mouse early development.2004

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biochem 135

      Pages: 165-169

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Diverse and essential roles of mammalian vacuolar-type proton pump ATPase : Toward physiological understanding of inside acidic compartments.2004

    • Author(s)
      M, Futai.
    • Journal Title

      Biochim.Biophys.Acta 1658

      Pages: 106-114

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mouse proton pump ATPase C subunit Isoforms (C2-a and C2-b) specifically expressed in kidney and lung.2004

    • Author(s)
      Masamistu Futai., Ge-Hong Sun-Wada
    • Journal Title

      J. Biol. Chem. 278

      Pages: 44843-44851

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regulatory elements, directing gut-specific expression of GATA6 gene during mouse early development.2004

    • Author(s)
      Masamistu Futai., Ge-Hong Sun-Wada
    • Journal Title

      J. Biochem. 135

      Pages: 165-169

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Diverse and essential roles of mammalian vacuolar-type proton pump ATPase : Toward physiological understanding of inside acidic compartments.2004

    • Author(s)
      Masamistu Futai., Ge-Hong Sun-Wada
    • Journal Title

      Biochim. Biophys. Acta. 1658

      Pages: 106-114

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Diversity of mouse proton-translocating ATPase : Presence of multiple isoforms of the C, d and G subunits.2003

    • Author(s)
      M, Futai.
    • Journal Title

      Gene. 302

      Pages: 147-153

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Subunit rotation in vacuolar-type proton pumping ATPase : relative rotation of G as to c subunit.2003

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 23714-23719

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Vacuolar H^+ pumping ATPase in luminal acidic organelles and extracellular compartments : Common rotational mechanism and diverse physiological roles2003

    • Author(s)
      M, Futai.
    • Journal Title

      J.Bioenerg.Biomemb. 35

      Pages: 347-358

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Specific recognition of Leishmania major poly-β-galactosyl epitopes by galectm-9 : Possible implication of galectm-9 in interaction between2003

    • Author(s)
      M, Futai.
    • Journal Title

      L.major and host cells J.Biol.Chem. 278

      Pages: 22223-22230

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Involvement of syntaxin 7 in human gastric epithelial cell vacuolation induced by the Helicobacter pylori-produced cytotoxin VacA.2003

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 25585-25590

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] From lysosomes to plasma membrane : Localization of vacuolar type H^+-ATPase with the a3 isoform during osteoclast differentiation.2003

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 278

      Pages: 22023-22030

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The vacuolar-type H^+-ATPase : a revised nomenclature for Mammalian vacuolar-type H^+-ATPase subunit genes.2003

    • Author(s)
      M, Futai.
    • Journal Title

      Mol. Cell 12

      Pages: 801-803

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection of protein-protein interactions on SiO_2/Si surface by spectroscopic ellipsometry.2003

    • Author(s)
      M, Futai.
    • Journal Title

      Anal.Biochem. 312

      Pages: 65-70

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Lysosome and lysosome-related organelles responsible for specialized functions in higher organisms.2003

    • Author(s)
      M, Futai.
    • Journal Title

      Cell Struct.Func. 28

      Pages: 455-463

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Diversity of mouse proton-translocating ATPase : Presence of multiple isoforms of the C, d and G subunits.2003

    • Author(s)
      Masamistu Futai
    • Journal Title

      Gene 302

      Pages: 147-153

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Subunit rotation in vacuolar-type proton pumping ATPase : relative rotation of G as to c subunit.2003

    • Author(s)
      Masamistu Futai
    • Journal Title

      J. Biol. Chem. 278

      Pages: 23714-23719

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Vacuolar H+ pumping ATPase in luminal acidic organelles and extracellular compartments : Common rotational mechanism and diverse physiological roles2003

    • Author(s)
      Masamistu Futai., Ge-Hong Sun-Wada
    • Journal Title

      J. Bioenerg. Biomemb. 35

      Pages: 347-358

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Involvement of syntaxin 7 in human gastric epithelial cell vacuolation induced by the Helicobacter pylori produced cytotoxin VacA.2003

    • Author(s)
      Masamistu Futai
    • Journal Title

      J. Biol. Chem. 278

      Pages: 25585-25590

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] From lysosomes to plasma membrane : Localization of vacuolar type H+ATPase with the a3 isoform during osteoclast differentiation.2003

    • Author(s)
      Masamistu Futai
    • Journal Title

      J. Biol. Chem. 278

      Pages: 22023-22030

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The vacuolar-type H^+-ATPase : a revised nomenclature for mammalian vacuolar-type H^+-ATPase subunit genes.2003

    • Author(s)
      Masamistu Futai
    • Journal Title

      Mol. Ce11. 12

      Pages: 801-803

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Detection of protein-protein interactions on Si0_2/Si surface by spectroscopic ellipsometry.2003

    • Author(s)
      Masamistu Futai
    • Journal Title

      Anal. Biochem. 321

      Pages: 65-70

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Lysosome and lysosome-related organelles responsible for specialized functions in higher organisms.2003

    • Author(s)
      Masamistu Futai., Ge-Hong Sun-Wada
    • Journal Title

      Cell Struct. Func. 28

      Pages: 455-463

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Sodium and sulfate ion transport in yeast vacuole2002

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biochem. 131

      Pages: 261-265

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A proton pump ATPase with testis-specific E1 subunit isoform Required for acrosome acidification2002

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 277

      Pages: 18098-18105

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A novel water-soluble hantzsh 1, 4-dihydropyridine compound that functions in biological processes through NADH2002

    • Author(s)
      M, Futai.
    • Journal Title

      J.Org.Chem. 10

      Pages: 3499-3501

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A human gene, ATP6E1, encoding a testis-specific isoform of H^+ATPase subunit E2002

    • Author(s)
      M, Futai.
    • Journal Title

      Gene. 289

      Pages: 7-12

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differential localization of V-type H+ pump with γ subunit isoforms (G1 and G2) in mouse neurons.2002

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 277

      Pages: 36296-36303

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Subunit rotation of ATP synthase embedded in membranes : a or β subunit rotation relative to the c subunit ring.2002

    • Author(s)
      M, Futai.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 99

      Pages: 13448-13452

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Sodium and sulfate ion transport in yeast vacuole,2002

    • Author(s)
      Masamistu Futai., Yo Wada
    • Journal Title

      J. Biochem. 131

      Pages: 261-265

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A novel water-soluble hantzsh 1, 4-dihydropyridine compound that functions in biological processes through NADH2002

    • Author(s)
      Masamistu Futai
    • Journal Title

      J. Org. Chem. 10

      Pages: 3499-3501

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A human gene, ATP6E1, encoding a testis-specific isoform of H^+ATPase subunit E2002

    • Author(s)
      Masamistu Futai
    • Journal Title

      Gene 289

      Pages: 7-12

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Differential localization of V-type H+ pump with y subunit isoforms (G1 and G2) in mouse neurons.2002

    • Author(s)
      Masamistu Futai
    • Journal Title

      J. Biol. Chem. 277

      Pages: 36296-36303

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Subunit rotation of ATP synthase embedded in membranes : a or β2002

    • Author(s)
      Masamistu Futai
    • Journal Title

      Proc. Natl. Acad. Sci. USA 99

      Pages: 13448-13452

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Rotation of a complex of the γ subunit and c ring of Escherichia coli ATP synthase : the rotor and stator are interchangeable.2001

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 276

      Pages: 15269-15274

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Association of mouse sorting nexin 1 oligomer with early endosomes.2001

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biochem. 130

      Pages: 765-7771

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Four Subunit a Isoforms of Caenorhabditis elegans vacuolar H^+-ATPase : Cell-specific expression during development.2001

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 276

      Pages: 33079-33085

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mouse Atp6f, the gene encoding the 23-kDa proteolipid of vacuolar proton translocating ATPase.2001

    • Author(s)
      M, Futai.
    • Journal Title

      Gene. 274

      Pages: 93-99

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ATP synthase F_1 sector rotation; defective torque generation in the b subunit Ser-174 to Phe mutant and its suppression by second mutations.2001

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 276

      Pages: 47508-47511

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The murine genome contain one functional gene and two pseudomones coding for the 16kDa proteolipid subunit of vacuolar H+ -ATPase2001

    • Author(s)
      K, Son.
    • Journal Title

      Gene 273

      Pages: 199-206

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] a4, a unique kidney-specific isoform of mouse vacuolar H^+-ATPase subunit a2001

    • Author(s)
      M, Futai.
    • Journal Title

      J.Biol.Chem. 276

      Pages: 40050-40054

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Rotation of a complex of the y subunit and c ring of Escherichia coli ATP synthase : the rotor and stator are interchangeable.2001

    • Author(s)
      Masamistu Futai
    • Journal Title

      J. Biol. Chem. 276

      Pages: 15269-15274

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Association of mouse sorting nexin 1 oligomer with early endosome2001

    • Author(s)
      Masamistu Futai Ge-Hong Sun-Wada
    • Journal Title

      J. Biochem. 130

      Pages: 765-7771

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Four Subunit a Isoforms of Caenorhabditis elegansvacuolar H+-ATPase : Cell-specific expression during development.2001

    • Author(s)
      Masamistu Futai
    • Journal Title

      J Biol. Chem. 276

      Pages: 33079-33085

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mouse Atp6f, the gene encoding the 23-kDa proteolipid of vacuolar proton translocating ATPase.2001

    • Author(s)
      Masamistu Futai., Ge-Hong Sun-Wada
    • Journal Title

      Gene 274

      Pages: 93-99

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ATP synthase Fi sector rotation; defective torque generation in the b subunit Ser 174 to Phe mutant and its suppression by second mutations2001

    • Author(s)
      Masamistu Futai
    • Journal Title

      J. Biol. Chem. 276

      Pages: 47508-47511

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The murine genome contain one functional gene and two pseudomonas coding for the 16kDa proteolipid subunit of vacuolar H+-ATPase,2001

    • Author(s)
      Ge-Hong Sun-Wada
    • Journal Title

      Gene 273

      Pages: 199-206

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] a4, a unique kidney-specific isoform of mouse vacuolar H^+-ATPase subunit a,2001

    • Author(s)
      Masamistu Futai Ge-Hong Sun-Wada
    • Journal Title

      J. Biol. Chem. 276

      Pages: 40050-40054

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A proton pump ATPase with testis-specific El subunit isoform required for acrosome acidification,2001

    • Author(s)
      Masamistu Futai., Ge-Hong Sun-Wada
    • Journal Title

      J. Biol. Chem. 277

      Pages: 18098-18105

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Specific recognition of Leishmania major poly-β-galactosyl epitopes by galectin-9 : Possible implication of galectin-9 in interaction between

    • Author(s)
      Masamistu Futai
    • Journal Title

      L. major and host cells J. Biol. Chem. 278

      Pages: 22223-22230

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Vacuolar-type proton ATPases : subunit isoforms and tissue-specific function. In "Handbook of ATPases : Biochemistry, Cell Biology, Pathophysiology"(Ed.by M.Futai, Y.Wada, J.Kaplan)2004

    • Author(s)
      M, Futai.
    • Total Pages
      24
    • Publisher
      Wiley-VCH Verlag GmbH & Co. KgaA, Weinheim, Germany
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Vacuolar-type proton ATPases : subunit isoforms and tissue-specific function. In "Handbook of ATPases Biochemistry, Cell Biology, Pathophysiology"(Ed.by M.Futai, Y.Wada, J.Kaplan)2004

    • Author(s)
      M, Futai.
    • Total Pages
      16
    • Publisher
      Wiley-VCH Verlag GmbH & Co. KgaA, Weinheim, Germany
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Vacuolar-type proton ATPases : subunit isoforms and tissue-specific function. In "Handbook of ATPases Biochemistry, Cell Biology, Pathophysiology"2004

    • Author(s)
      Masamistu Futai., Yo Wada
    • Total Pages
      24
    • Publisher
      (Ed. by M. Futai, Y. Wada and J. Kaplan) Wiley-VCH Verlag GmbH & Co. KgaA, Weinheim, Germany
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Vacuolar-type proton ATPases : subunit isoforms and tissue-specific function. In "Handbook of ATPases : Biochemistry, Cell Biology, Pathophysiology"2004

    • Author(s)
      Masamistu Futai., Ge-Hong Sun-Wada
    • Total Pages
      16
    • Publisher
      (Ed. by M. Futai, Y. Wada and J. Kaplan) Wiley-VCH Verlag GmbH & Co. KgaA, Weinheim, Germany
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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