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2005 Fiscal Year Final Research Report Summary

Role for protease cascade in humaral and cellular immunity of insects

Research Project

Project/Area Number 13143201
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionHOKKAIDO UNIVERCTIY

Principal Investigator

ASHIDA Masaaki  Hokkaido University, Institute of Low Temperature Science, Professor, 低温科学研究所, 教授 (50012422)

Co-Investigator(Kenkyū-buntansha) HAYAKAWA Yoichi  Hokkaido University, Institute of Low Temperature Science, Associated Professor, 低温科学研究所, 助教授 (50164926)
KATAGIRI Chihiro  Hokkaido University, Institute of Low Temperature Science, Instructor, 低温科学研究所, 助手 (90002245)
OCHIAI Masanori  Hokkaido University, Institute of Low Temperature Scienoe, Instructor, 低温科学研究所, 助手 (10241382)
Project Period (FY) 2001 – 2003
Keywordsinnate immunity / defense system / non-self recognition / protease cascade / Growth-blocking peptide / prophenoloxidase cascade / cytokine
Research Abstract

Growth-blocking peptide (GBP) is a 25 amino acid insect cytokine found in Lepidopteran insects that possesses diverse biological activities such as larval growth regulation and stimulation of immune cells. We identified homologous peptides of the Lepidopteran GBP in the other orders of insects. Two novel cytokines were purified from coleopteran and dipteran larvae. Homology search of the Drosophila melanogaster and Anopheles gambiae genome database for the fly cytokine revealed three drosophila and two mosquito homologues, suggesting wide-spread occurrence of this cytokine family. Furthermore, a GBP-binding protein was isolated from Lepidopteran. Kinetic and biological analyses of interaction between GBP and the binding protein showed their strong binding regulates cellular immunoreaction through the stimulation of completely different types of the cells.
The prophenoloxidase (proPO) cascade of insect hemolymph plays some important roles in the recognition of foreignness and defense mec … More hanism. The cascade is triggered by microbial cell wall components such as β-1,3-glucan from fungi and peptidoglycan from bacteria, resulting in localized melanization. To investigate the mechanism for the activation of the cascade, we identified three new components (βGRPII, GNBP, Factor S) of the silkworm proPO cascade. βGRPII and GNBP are involved in β-1,3-glucan-and peptidoglycan- mediated pathways of the cascade, respectively. Factor S is a serine protease zymogen, which is located between the pattern recognition proteins and proBAEEase and is an essential component both in the proPO-activating pathway for melanin synthesis and in proBAEEase-activating pathway. Moreover, BAEEase was shown to process Drosophila pro-spatzle to spatzle, which is a cytokine-like ligand on Toll-mediated pathway for control of the synthesis of some anti-microbial peptides in Drosophila immunity. These results suggests that the proPO cascade of insects is an extracellular signaling mechanism for recognizing PAMPs as foreignness in the induction of acute-phase protein synthesis, not only an effector mechanism for the production of melanin. Less

  • Research Products

    (36 results)

All 2003 2002 2001

All Journal Article (34 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Role of aromatic residues in the structure and biological activity of the small cytokine, growth-blocking peptide2003

    • Author(s)
      Tada, M. et al.
    • Journal Title

      J. Biol. Chem. 278

      Pages: 10778-10783

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Isolation and characterization of a dopa decarboxylase cDNA and the induction of its expression by an insect cytokine, growth-blocking peptide in Pseuadaletia separata2003

    • Author(s)
      Naguchi, H. et al.
    • Journal Title

      Insect Biochem. Bolec. Biol. 33

      Pages: 209-217

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of polydnavirus genes in insect hemocyte and Sf9 culture cells2003

    • Author(s)
      Tanaka, K. et al.
    • Journal Title

      J. Insect Physiol. 49

      Pages: 433-440

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The cause of mortality in insects under severs stress2003

    • Author(s)
      Matsumoto, H. et al.
    • Journal Title

      Eur. J. Biolchem. 270

      Pages: 3469-3476

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Insect cytokine, growth-blocking peptide, triggers off a ter mination system of cellular immunity by inducing its binding protein2003

    • Author(s)
      Matsumoto, Y. et al.
    • Journal Title

      J. Biol. Chem. 278

      Pages: 38579-38585

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Role of aromatic residues in the structure and biological activity of the small cytokine, growth-blocking peptide.2003

    • Author(s)
      Tada, M.
    • Journal Title

      J Biol. Chem. 278

      Pages: 10778-10783

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Isolation and characterization of a dopa decarboxylase cDNA and the induction of its expression by an insect cytokine, growth-blocking peptide in Pseuadaletia separata.2003

    • Author(s)
      Noguchi, H.
    • Journal Title

      Insect Biochem. Molec. Biol. 33

      Pages: 209-217

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of polydnavirus genes in insect hemocyte and Sf9 culture cells.2003

    • Author(s)
      Tanaka, K.
    • Journal Title

      J Insect P.hysiol. 49

      Pages: 433.440

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The cause of mortality in insects under severe stress.2003

    • Author(s)
      Matsumoto, H.
    • Journal Title

      Eur. J. Biochem. 270

      Pages: 3469-3470

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Insect cytokine, growth-blocking peptide, triggers off a termination system of cellular immunity by inducing its binding protein.2003

    • Author(s)
      Matsumoto, Y.
    • Journal Title

      J. Biol. Chem. 278

      Pages: 38579-38585

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Existence of phenol oxidase in the argasid tick Ornithodoros Moubata2002

    • Author(s)
      Kadota, K. et al.
    • Journal Title

      Parasitol Res. 88

      Pages: 781-784

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Solution structure of paralytic peptide of silkworm, Bombyx mori2002

    • Author(s)
      Miura, K. et al.
    • Journal Title

      Peptides 23

      Pages: 2111-2116

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Structure and activity of insect cytokine GBP which stimulates the EGF receptor2002

    • Author(s)
      Aizawa, T. et al.
    • Journal Title

      Mol. Cells 14

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression and purification of a small cytokine growth-blocking peptide from armyworm Pseudaletia separata by an optimised fermentation method using the methylotrophic yeast Pichia pastoris2002

    • Author(s)
      Koganesawa, N. et al.
    • Journal Title

      Protein Expression and Purification 25

      Pages: 416-425

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detailed characterization of polydnavirus immunoevasive proteins in an endoparasitoid wasp2002

    • Author(s)
      Tanaka, K. et al.
    • Journal Title

      Eur. J. Biochem. 269

      Pages: 2557-2566

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Analysis in the course of polydnavirus replication in ovarian calyx cells of parasitoid wasp2002

    • Author(s)
      Tanaka, K. et al.
    • Journal Title

      Appl. Entomol. Zool. 37

      Pages: 323-328

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Existence of phenol oxidase in the argasid tick Ornithodoros moubata.2002

    • Author(s)
      Kadota, K.
    • Journal Title

      Parasitol Res. 88

      Pages: 781-784

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Solution structure of paralytic peptide of silkworm, Bombyx mori.2002

    • Author(s)
      Miura, K.
    • Journal Title

      Peptides 23

      Pages: 2111-2116

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Structure and activity of insect cytokine GBP which stimulates the EGF receptor.2002

    • Author(s)
      Aizawa, T.
    • Journal Title

      Mol. Cells 14

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression and purification of a small cytokine growth-blocking peptide from armyworm Pseudaletia separata by an optimised fermentation method using the methylotrophic yeast Pichia pastoris.2002

    • Author(s)
      Koganesawa, N.
    • Journal Title

      Protein Expres. Purif 25

      Pages: 416-425

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Detailed characterization of polydnavirus immunoevasive proteins in an endoparasitoid wasp.2002

    • Author(s)
      Tanaka, K.
    • Journal Title

      Eur. J. Biochem. 269

      Pages: 2557-2566

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Analysis in the course of polydnavirus replication in ovarian calyx cells of parasitoid wasp.2002

    • Author(s)
      Tanaka, K.
    • Journal Title

      Appl. Entomol. Zool. 37

      Pages: 323-328

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] N-terminal residues of plamatocyte spreading peptide posses s specific determinants required for biological activity2001

    • Author(s)
      Clark, K.D.et al.
    • Journal Title

      J. Biol. Chem. 276

      Pages: 37431-37435

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Structure and activity of the insect cytokine growth-blocking peptide2001

    • Author(s)
      Aizawa, T. et al.
    • Journal Title

      J. Biol. Chem. 276

      Pages: 31813-31818

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Prominent immunogenicity of monosialosyl galactosylgloboside, carrying a stage-specific embryonic antigen-4 (SSEA-4) epitope in the ACHN human renal tubular cell line - a simple method for producing monoclonal antibodies against detergent incoluble microdomains/raft2001

    • Author(s)
      Katagiri, Y. et al.
    • Journal Title

      Glycoconjugate J. 18

      Pages: 347-353

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transepithelially transported pro-phenoloxidase in the cuticle of the silkorm, Bombyx mori : identification of its methy onyl residues oxidized to methionine sulfoxides2001

    • Author(s)
      Asano, T. et al.
    • Journal Title

      J. Biol. Chem. 276

      Pages: 11113-11125

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cuticular pro-phenoloxidase of the silkworm, Bombyx mori : purification and demonstration of its transport from hemolymph2001

    • Author(s)
      Asano, T. et al.
    • Journal Title

      J. Biol. Chem. 276

      Pages: 11100-11112

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Alanine-scanning mutagenesis of plasmatocyte spreading peptide identifies critical residues for biological activity2001

    • Author(s)
      Clark, K.D.et al.
    • Journal Title

      J. BNiol. Chem. 276

      Pages: 18491-18496

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] N-terminal residues of plasmatocyte spreading peptide possess specific determinants required for biological activity.2001

    • Author(s)
      Clark, K. D.
    • Journal Title

      J. Biol. Chem. 276

      Pages: 37431-37435

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Structure and activity of the insect cytokine growth-blocking. peptide.2001

    • Author(s)
      Aizawa, T.
    • Journal Title

      J. Biol. Chem. 276

      Pages: 31813-31818

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Prominent immunogenicity of monosialosyl galactosylgloboside, carrying a stage-specific embryonic antigen-4 (SSEA-4) epitope in the ACHN human renal tubular cell line-a simple method for producing monoclonal antibodies against detergent insoluble microdomains/raft.2001

    • Author(s)
      Katagiri, Y. U.
    • Journal Title

      Glycoconjugate J. 18

      Pages: 347-353

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Transepithelially transported pro-phenoloxidase in the cuticle of the silkworm, Bombyx mori : identification of its methyonyl residues oxidized to methionine sulfroddes.2001

    • Author(s)
      Asano, T.
    • Journal Title

      J Biol. Chem. 276

      Pages: 11113-11125

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cuticular pro-phenoloxidase of the silkworm, Bombyx mori : purification and demonstration of its transport from hemolymph.2001

    • Author(s)
      Asano, T.
    • Journal Title

      J. BioL Chem. 276

      Pages: 11100-11112

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Alanine-scanning mutagenesis of plasmatocyte spreading peptide identifies critical residues for biological activity.2001

    • Author(s)
      Clark, K. D.
    • Journal Title

      J. Biol. Chem. 276

      Pages: 18491-18496

    • Description
      「研究成果報告書概要(欧文)」より
  • [Patent(Industrial Property Rights)] 昆虫から単離した新規生理活性ペプチド2003

    • Inventor(s)
      早川洋一
    • Industrial Property Rights Holder
      同左
    • Industrial Property Number
      特許第3718720号
    • Filing Date
      2003-05-23
    • Acquisition Date
      2005-09-16
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 昆虫培養支持因子を調製する方法2002

    • Inventor(s)
      早川洋一
    • Industrial Property Rights Holder
      同左
    • Industrial Property Number
      特願2002-104118号
    • Filing Date
      2002-04-05
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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