2001 Fiscal Year Final Research Report Summary
Clinical significance of polymorphism in cGMP-related genes on blood pressure control in humans.
| Project/Area Number |
13204043
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyoto University |
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Principal Investigator |
ITOH Hiroshi Kyoto University, Graduate School of Medicine, Associate Professor, 医学研究科, 助教授 (40252457)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAO Kazuwa Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (00172263)
INOUE Gen Kyoto University, Graduate School of Medicine, Assistant professor, 医学研究科, 講師 (20260606)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Hypertension / genome / natriuretic peptide / GC-A / allele frequency |
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| Research Abstract |
The purpose of this study was to evaluate the significance of the effect of cGMP-related gene on controlling blood pressure in humans. After the informed consents, blood sample was drawn from normotensive (n=180, mean blood pressure 124/74 mmHg) and hypertensive (n=189, mean blood pressure 162/93 mmHg). Extracted genome was subjected to PCR direct sequence method to detect polymorphism in the sequence of guanylyl cyclase-A gene, which is one of the two major types of receptors for natriuretic peptides. As a result, we had detected 5 polymorphisms through the whole span of 22 exsons and 21 introns which length is 16kb. In this study, we investigated one of those polymorphisms, CT repeats in-300 bp of 5' franking lesion of this gene. We detected the existence of (CT) 6 repeats in addition to already reported (CT) 10, 11, 12 repeats. More over, the allele frequency of (CT)6 seems to be significantly different between normotensive and hypertensive. On the other hand, there seems to be no significant difference in the allele frequency of (CT) 10, 11, 12 repeats. In addition to human data described upon, we analyzed guanylyl cyclase-A knock out mice and revealed the significance of the natriuretic peptide system in gender-related cardiac hypertrophy and fibrosis.
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