2003 Fiscal Year Annual Research Report

ナトリウム利尿ペプチドは魚類の広塩性を支配するか:トランスジェニック法による解析

Research Project

Project/Area Number	13304063
Research Institution	The University of Tokyo
Principal Investigator	竹井祥郎東京大学, 海洋研究所, 教授 (10129249)
Co-Investigator(Kenkyū-buntansha)	井上広滋東京大学, 海洋研究所, 助手 (60323630) 兵藤晋東京大学, 海洋研究所, 助教授 (40222244) 豊原治彦京都大学, 大学院・農学研究科, 助教授 (90183079)
Keywords	ナトリウム利尿ペプチド / グアニリン / アドレノメデュリン / 分子進化 / 体液調節作用 / 心血管作用 / ウナギ,Anguiila japonica / トラフグ,Takifugu rubripes
Research Abstract	本年度は、ナトリウム利尿ペプチド(NP)ファミリーの分子進化と、新しい浸透圧調節ホルモンである可能性を持つアドレノメデュリンファミリーの発見で大きな発展があった。 1)NPファミリーは、これまで魚類ではANP, VNP, CNPの3種がファミリーを形成していると考えられてきたが、私たちはチョウザメでANP, BNP, VNP, CNPが共存することを明らかにした。その結果、ほ乳類で知られていたBNPと硬骨魚類の真骨類で知られていたVNPが別物であることがわかった。そこで真骨類でBNPを探したところ、ティラピア、メダカ、フグ、キリフィッシユ、ヒラメなどでBNPを見つけることができた。また、真骨類には4種のCNPが存在し、ほ乳類のCNPはCNP-4であり、CNP-3からANPとBNPがタンデムに遺伝子重複により作られたことを明らかにした。いっぽう、もつとも原始的な脊椎動物である円口類に属するヤツメウナギには、CNP-4のみが存在することが明らかになった。したがって、NPファミリーの祖先分子はCNP-4であり、硬骨魚類へと進化する過程で大規模な多様化が起こった可能性が高い。 2)ほ乳類で多機能ホルモンとして知られているアドレノメデュリン(AM)が、フグでは5つのパラログからなるAMファミリーを作っていることを明らかにした。5種のうち、フグAM-1がほ乳類AMのオルソログであることがリンケージ解析により明らかになった。いっぽう、AM-1/4/5とAM-2/3が異なるグループに属することがエキソン・イントロン解析や系統解析で示唆されたため、ほ乳類でAM-2を探したところ、ヒト、マウス、ラットでAM-2を発見することができた。AM-2は強力な心血管作用や腎作用を持つため、血圧や体液を調節する主要なホルモンであると考えられる。

Research Products

(22 results)

All Other

All Publications (22 results)

[Publications] 岩谷芳自他: "異なる環境浸透圧で飼育したカマキリ(Cottus kazaka)の成長と生残率"日本水産学会誌. 69. 52-56 (2003)
[Publications] Balment R.J.et al.: "Isolation, synthesis and biological activity of flounder [Asn1, Ile5,Thr9] angiotensin I"General and Comparative Endocrinology. 130. 92-98 (2003)
[Publications] Inoue K.et al.: "C-type natriuretic peptide of rainbow trout (Oncorhynchus mykiss) : primary structure and vasorelaxant activities"General and Comparative Endocrinology. 130. 185-192 (2003)
[Publications] Li, Y.-Y.et al.: "Ambient salinity-dependent effects of homologous natriuretic peptides (ANP, VNP and CNP) on plasma cortisol levels in the eel"General and Comparative Endocrinology. 130. 317-323 (2003)
[Publications] Eckert S.M.et al.: "Effects of angiotensin II and natriuretic peptides on prolactin and growth hormone release in the tilapia, Oreochromis mossambics"General and Comparative Endocrinology. 130. 333-339 (2003)
[Publications] Li Y.-Y.et al.: "Steroidogenic acute regulatory protein in eels: characterization of mRNA and its regulation by ACTH and seawater transfer"Zoological Science. 20. 211-219 (2003)
[Publications] Yuge S.et al.: "A novel guanylin family (guanylin, uroguanylin and renoguanylin) in eel : possible osmoregulatory hormones in intestine and kidney"Journal of Biological Chemistry. 278. 22726-22733 (2003)
[Publications] Inoue K.et al.: "Growth hormone and insulin-like growth factor I of a euryhaline sculpin, Cottus kazaka"General and Comparative Endocrinology. 131. 77-84 (2003)
[Publications] Kawakoshi, A.et al.: "A novel natriuretic peptide that is exclusively expressed in the heard and brain of the most primitive extant vertebrate, the hagfish"Journal of Molecular Endocrinology. 31. 209-220 (2003)
[Publications] Inoue K.et al.: "Four functionally distinct C-type natriuretic peptides found in fish reveal new evolutionary history of the natriuretic system"Proceedings of the national Academy of Sciences of the USA. 100. 10079-10084 (2003)
[Publications] Li Y.-Y.et al.: "Interrenal steroid 21-hydroxylase in eels : primary structure, progesterone-specific activity and enhanced expression by ACTH"Journal of Molecular Endocrinology. 31. 327-340 (2003)
[Publications] Takei Y.: "A collection from the international symposium on "Function of Marine Organisms-Mechanisms of adaption to diverse environments""Comparative Biochemistry and Physiology. 136B. 575 (2003)
[Publications] Hirose S.et al.: "Molecular biology of major components of chloride cells"Comparative Biochemistry and Physiology. 136B. 593-620 (2003)
[Publications] Inoue K.et al.: "Asian medaka fishes offer new models for studying seawater adaptation"Comparative Biochemistry an Physiology. 136B. 635-645 (2003)
[Publications] Ogoshi M.et al.: "Identification of a novel adrenomedullin gene family in teleost fish"Biochemical and Biophysical Research Comm. 311. 1072-1077 (2003)
[Publications] 岩谷芳自他: "カマキリ(Cottus kazika)仔稚魚期の淡水耐性"SUISANZOUSHOKU. 51. 423-428 (2003)
[Publications] Takei Y.et al.: "Mammalian homolog of fish adrenomedullin 2 : Identification of a novel cardiovascular and renal regulator"Federation of European Biochemical Societies Letters. 556. 53-58 (2003)
[Publications] Hyodo S.et al.: "A facilitative urea transporter is localized in the renal collecting tubule of dogfish, Triakis scyllia"Journal of Experimental Biology. 207. 347-356 (2004)
[Publications] Rankin J.C.et al.: "Identification of angiotensin I in a cyclostome, Lampetra fluvatilis"Zoological Science. 21. 173-179 (2004)
[Publications] Takei Y.et al.: "Identification of angiotensin I from several vertebrate species : Its structural and functional evolution"General and Comparative Endocrinology. 135. 286-292 (2004)
[Publications] Kawakoshi A.et al.: "Four natriuretic peptides (ANP, BNP, VNP and CNP) coexist in the sturgeion New evidence for the presence of BNP in fish lineage"Journal of Molecular Endocrinology. (in Press).
[Publications] 竹井祥郎: "動物生理学"朝倉書店. 61 (2003)

2003 Fiscal Year Annual Research Report

ナトリウム利尿ペプチドは魚類の広塩性を支配するか:トランスジェニック法による解析

Principal Investigator

竹井 祥郎 東京大学, 海洋研究所, 教授 (10129249)

Research Products

[Publications] 岩谷芳自 他: "異なる環境浸透圧で飼育したカマキリ(Cottus kazaka)の成長と生残率"日本水産学会誌. 69. 52-56 (2003)

[Publications] Balment R.J.et al.: "Isolation, synthesis and biological activity of flounder [Asn1, Ile5,Thr9] angiotensin I"General and Comparative Endocrinology. 130. 92-98 (2003)

[Publications] Inoue K.et al.: "C-type natriuretic peptide of rainbow trout (Oncorhynchus mykiss) : primary structure and vasorelaxant activities"General and Comparative Endocrinology. 130. 185-192 (2003)

[Publications] Li, Y.-Y.et al.: "Ambient salinity-dependent effects of homologous natriuretic peptides (ANP, VNP and CNP) on plasma cortisol levels in the eel"General and Comparative Endocrinology. 130. 317-323 (2003)

[Publications] Eckert S.M.et al.: "Effects of angiotensin II and natriuretic peptides on prolactin and growth hormone release in the tilapia, Oreochromis mossambics"General and Comparative Endocrinology. 130. 333-339 (2003)

[Publications] Li Y.-Y.et al.: "Steroidogenic acute regulatory protein in eels: characterization of mRNA and its regulation by ACTH and seawater transfer"Zoological Science. 20. 211-219 (2003)

[Publications] Yuge S.et al.: "A novel guanylin family (guanylin, uroguanylin and renoguanylin) in eel : possible osmoregulatory hormones in intestine and kidney"Journal of Biological Chemistry. 278. 22726-22733 (2003)

[Publications] Inoue K.et al.: "Growth hormone and insulin-like growth factor I of a euryhaline sculpin, Cottus kazaka"General and Comparative Endocrinology. 131. 77-84 (2003)

[Publications] Kawakoshi, A.et al.: "A novel natriuretic peptide that is exclusively expressed in the heard and brain of the most primitive extant vertebrate, the hagfish"Journal of Molecular Endocrinology. 31. 209-220 (2003)

[Publications] Inoue K.et al.: "Four functionally distinct C-type natriuretic peptides found in fish reveal new evolutionary history of the natriuretic system"Proceedings of the national Academy of Sciences of the USA. 100. 10079-10084 (2003)

[Publications] Li Y.-Y.et al.: "Interrenal steroid 21-hydroxylase in eels : primary structure, progesterone-specific activity and enhanced expression by ACTH"Journal of Molecular Endocrinology. 31. 327-340 (2003)

[Publications] Takei Y.: "A collection from the international symposium on "Function of Marine Organisms-Mechanisms of adaption to diverse environments""Comparative Biochemistry and Physiology. 136B. 575 (2003)

[Publications] Hirose S.et al.: "Molecular biology of major components of chloride cells"Comparative Biochemistry and Physiology. 136B. 593-620 (2003)

[Publications] Inoue K.et al.: "Asian medaka fishes offer new models for studying seawater adaptation"Comparative Biochemistry an Physiology. 136B. 635-645 (2003)

[Publications] Ogoshi M.et al.: "Identification of a novel adrenomedullin gene family in teleost fish"Biochemical and Biophysical Research Comm. 311. 1072-1077 (2003)

[Publications] 岩谷芳自 他: "カマキリ(Cottus kazika)仔稚魚期の淡水耐性"SUISANZOUSHOKU. 51. 423-428 (2003)

[Publications] Takei Y.et al.: "Mammalian homolog of fish adrenomedullin 2 : Identification of a novel cardiovascular and renal regulator"Federation of European Biochemical Societies Letters. 556. 53-58 (2003)

[Publications] Hyodo S.et al.: "A facilitative urea transporter is localized in the renal collecting tubule of dogfish, Triakis scyllia"Journal of Experimental Biology. 207. 347-356 (2004)

[Publications] Rankin J.C.et al.: "Identification of angiotensin I in a cyclostome, Lampetra fluvatilis"Zoological Science. 21. 173-179 (2004)

[Publications] Takei Y.et al.: "Identification of angiotensin I from several vertebrate species : Its structural and functional evolution"General and Comparative Endocrinology. 135. 286-292 (2004)

[Publications] Kawakoshi A.et al.: "Four natriuretic peptides (ANP, BNP, VNP and CNP) coexist in the sturgeion New evidence for the presence of BNP in fish lineage"Journal of Molecular Endocrinology. (in Press).

[Publications] 竹井祥郎: "動物生理学"朝倉書店. 61 (2003)

竹井祥郎東京大学, 海洋研究所, 教授 (10129249)

[Publications] 岩谷芳自他: "異なる環境浸透圧で飼育したカマキリ(Cottus kazaka)の成長と生残率"日本水産学会誌. 69. 52-56 (2003)

[Publications] 岩谷芳自他: "カマキリ(Cottus kazika)仔稚魚期の淡水耐性"SUISANZOUSHOKU. 51. 423-428 (2003)