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2003 Fiscal Year Final Research Report Summary

Smooth muscle regulation

Research Project

Project/Area Number 13307005
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionGunma University

Principal Investigator

KOHAMA Kazuhiro  Gunma University, Medicine, Professor, 医学部, 教授 (30101116)

Co-Investigator(Kenkyū-buntansha) KUMAGAI Hiroyuki  Gunma University, Medicine, Assistant, 医学部, 助手 (20321945)
NAKAMURA Akio  Gunma University, Medicine, Assistant, 医学部, 助手 (30282388)
ISHIKAWA Ryoki  Gunma University, Medicine, Lecturer, 医学部, 講師 (20212863)
Project Period (FY) 2001 – 2003
KeywordsSmooth muscle / regulation / myosin / myosin light chain kinase / down-regulation
Research Abstract

Myosin light chain kinase (MLCK) is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. Although this mode of action is well-established, there are numerous reports of smooth muscle contraction that is not associated with MLC20 phosphorylation. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal. The present study shows how such multifunctional properties of MLCK modify the actin-myosin interaction and presents our observations that the phosphorylation is not obligatory in induction of smooth muscle contraction.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] W.Iwasaki, H.Sasaki, A.Nakamura, K.Kohama, M.Tanokura: "Metal-free and Ca^<2+>-bound structures of a multi-domain EF-hand protein, CBP40, from the lower eukaryote Physarum polycephalum."Structure. 11. 75-85 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Gao, K.Kawano, S.Yoshiyama, H.Kawamiti, X.Wang, A.Nakamura, K.Kohama: "Myosin light chain kinae stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain."Biochem.Biophys.Res.Commun.. 305. 16-21 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] L.Farkas, A.Malnasi-Csizmadia, A.Nakamura, K.Kohama, L.Nyitray: "Localization and characterization of the inhibitory Ca^<2+>-binding site of Physarum polycephalum myosin II."J.Biol.Chem.. 278. 27399-27404 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Kato, E.Aihara, A.Nakamura, H.Xin, H.Matsui, K.Kohama, K.Takeuchi: "Expression of vanilloid receptors in rat gastric epithelial cells : role in cellular protection."Biochem.Pharmacol.. 66. 1115-1121 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sakamoto, M.Hori, M.Izumi, T.Oka, K.Kohama, H.Ozaki, H.Karaki: "Inhibition of high K^+-induced contraction by the ROCKs inhibitor Y-27632 in vascular smooth muscle : Possible involvement of ROCKs in a signal transduction pathway."J.Pharmacol.Sci.. 92. 56-69 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Ishikawa, T.Sakamoto, T.Ando, S.Higashi-Fujime, K.Kohama: "Polarized actin bundles formed by human fascin-1 : their sliding and disassembly on myosin II and myosin V in vitro."J.Neurochem.. 87. 676-685 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Li, T.Zhao, H.Xin, L.-H.Ye, Z.Zhang, H.Tanaka, A.Nakamura, K.Kohama: "Nicotinic acetylcholine receptor α7 subunit mediates migration of vascular smooth muscle cells toward nicotine."J.Pharmacol.Sci.. 94. 334-338 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] L.Farkas, A.Malnasi-Csizmadis, A.Nakamura, K.Kohama, L.Nyitray: "Localization and characterization of the inhibitory Ca^<2+>-binding site of Physarum polycephalum myosin II."J.Biol.Chem.. 278. 27399-27404 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Gao, Kawano, S.Yoshiyama, H.Kawamichi, X.Wang, A.Nakamura, K.Kohama: "Myosin light chain kinase stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain."Biophys.Res.Commun.. 305. 16-21 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Ishikawa, T.Sakamoto, T.Ando, S.Higashi-Fujime, K.Kohama: "Polarized actin bundles formed by human fascin-1 : their sliding and disassembly on myosin II and myosin V in Vitro."J.Neurochem.. 87. 676-685 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sakamoto, M.Hori, M.Izumi, T.Oka, K.Kohama, H.Ozaki, H.Karaki: "Inhibition of high K^+-induced contraction by the ROCKs inhibitor Y-27632 in vascular smooth muscle : Possible involvement of ROCKs in a signal transduction pathway."J.Pharmacol.Sci.. 92. 56-69 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] W.Iwasaki, H.Sakaki, A.Nakamura, K.Kohama, M.Tanakura: "Metal-free and Ca^<2+>-bound structures of a multi-domain EF-hand protein, CBP40, from the lower eukaryote Physarum polycephalum."Structure. 11. 75-85 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kato, E.Aihara, A.Nakamura, H.Xin, H.Matsui, K.Kohama, K.Takeuchi: "Expression of vanilloid receptors in rat gastric epithelial cells : role in cellular protection."Biochem.Pharmacol.. 66. 1115-1121 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Bao, K Oishi, T.Yamada, L.Liu, A.Nakamura, M.K.Uchida, K.Kohama: "Role of the short isoform of myosin light chain kinase in the contraction smooth muscle cells as by its down-regulation."Proc.Natl.Acad.Sci.USA. 99. 9556-9561 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Mimaki, S.Kagawa, M.Aoi, S.Kato, S.Thutumi, K.Kohama, K.Takeuchi: "Effect of lafutidine, a histamine H_2-receptor antagonist on gastric mucosal blood flow and duodanal HCO_3 secretion in rats : relation to capsaicin-sensitive afferent neurons."Digestive Diseases and Science. 47. 2696-2703 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Gao, L.-H.Ye, H.Kishi, I.Okagaki.K.Samizo, A.Nakamura, K.Kohama: "Myosin light chain kinase as a multifunctional regulatory protein of smooth muscle contraction."IUBMB Life. 51. 337-344 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Samizo, R.Ishikawa, A.Nakamura, K.Kohama: "A highly sensitive method for measurement of myosin ATPase activity by reversed-phase high-performance liquid chromatography."Anal.Biochem.. 293. 212-215 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Tsutumi, A.Tomioka, M.Sudo, A Nakamura, K.Shirakawa, K.Takegushi, K.Kohama: "Propofol activates vanilloid receptor channels expressed in human embryonic kidney 293 cells."Neurosci.Lett.. 312. 45-49 (2001)

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      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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