2003 Fiscal Year Final Research Report Summary
Nuclear transfer of mouse ES cells
| Project/Area Number |
13308050
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kinki University |
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Principal Investigator |
TSUNODA Yukio Kinki University, College of Agriculture, Professor, 農学部, 教授 (80217364)
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| Co-Investigator(Kenkyū-buntansha) |
TANI Tetsuya Kinki University, College of Agriculture, Research associate, 農学部, 助手 (70319763)
KATO Yoko Kinki University, College of Agriculture, Associate Professor, 農学部, 助教授 (40278742)
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| Project Period (FY) |
2001 – 2003
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| Keywords | ES cells / nuclear transfer / cloned mice |
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| Research Abstract |
Some of nuclear-transferred oocytes receiving mouse embryonic stem(ES) cells develop into fertile young. Although the developmental potential of nuclear-transferred oocytes that receive ES cells is high compared with those receive somatic cells, a large proportion die at various developmental stages and the rate of neonatal death is high. The present study examined the effect of 1)aggregation of nuclear-transferred oocytes to increase cell numbers, 2)genetic background of ES cells, 3)different nuclear transfer procedures, 4)cell cycle of ES cells, and 5)activation methods on cloning.
The following results were obtained. 1)The low potential of nuclear-transferred oocytes with ES cells to develop into young was not due to the lower cell number of nuclear transferred-embryos. 2)The cloning efficiency with hybrid ES cell lines was not superior to that of an inbred ES cell line. 3)The potential of nuclear-transferred oocytes to develop into blastocysts after fusion by Sendai virus was high compared with that after direct injection. 4)The developmental potential of oocytes receiving ES cells at the M phase was higher than that of oocytes receiving ES cells at the G1 phase. Different activation protocols did not affect the potential to develop into blastocysts. Since the developmental potential of nuclear-transferred oocytes to live pups was still low in all group(1 to 4%), further methodological studies to increase the viability of nuclear-transferred oocytes are required.
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