2003 Fiscal Year Final Research Report Summary
The study on the screen of genes for the regulation of cell differentiation using mutant mice created by heavy ion beams.
| Project/Area Number |
13308051
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | The Institute of physical and Chemical Research (RIKEN) |
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Principal Investigator |
YSHIKI Atsushi RIKEN, Experimental Animal Division, Senior Research Scientist, 実験動物開発室, 先任研究員 (40212310)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAIWA Noriko RIKEN, Experimental Animal Division, Senior Technical Scientist, 実験動物開発室, 先任技師(研究職) (30200380)
NORO Chikako RIKEN, Experimental Animal Division, Senior Technical Scientist, 実験動物開発室, 先任技師(研究職) (80311356)
IKE Fumio RIKEN, Experimental Animal Division, Senior Research Scientist, 実験動物開発室, 先任研究員 (40183157)
YANO Yasushige RIKEN, Chief Scientist, 加速器基盤研究部, 主任研究員
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| Project Period (FY) |
2001 – 2003
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| Keywords | heavy ion beam / mutagenesis / in vitro fertilization / embryonic development / cancer susceptibility / BAC CGH / Pseudogene / angiogenesis |
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| Research Abstract |
Recessive screen of fetal phenotypes in G3 embryos demonstrated that morphological anomalies including the neural tube disclosure and developmental retardation, suggesting the effectiveness of HIBs for mutant mice generation. We tested the feasibility of frozen-stored sperms after HIBs irradiation. The practical dosage of carbon, nitrogen and neon ions ranged from 1-5 Gy. In the dominant screen of skin cancer resistance genes indicated that the HIBs could induce deletion of the cancer resistance loci, resulting the cancer susceptible Fl hybrids. BAC CGH array can be useful to detect these deletion by the HIBs irradiation. We also explored the gene regulation mechanism in a transgenic mouse model and an angiogenesis chick model. We found a novel function of an expressed pseudogene in the transgenic mouse and important molecular mechanisms in angiogenesis. We will search mutations of these important genes and find the phenotype in the HIB-induced mutant mice in the future.
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