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2002 Fiscal Year Final Research Report Summary

Characterization of the functional role of zinc-finger motif in acetyle-CoA carboxylase

Research Project

Project/Area Number 13460040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionSaga University (2002)
Nagoya University (2001)

Principal Investigator

NAGANO Yukio  Saga University, Analytical Research Center for Experimental Sciences, Associate Professor, 総合分析実験センター, 助教授 (00263038)

Co-Investigator(Kenkyū-buntansha) SASAKI Yukiko  Genesis Research Institute, INC., Executive Research Scientist, 特別研究員
Project Period (FY) 2001 – 2002
Keywordsfatty acid synthesis / acetyl-CoA carboxylase / zinc-finger motif / metal ions / zinc ions / acetyl-CoA / malonyl-CoA / carboxyltransferase
Research Abstract

Acetyl-CoA carboxylase (ACCase) [EC 6.4.1.2] catalyzes the first committed step of fatty acid synthesis, namely the carboxylation of acetyl-CoA to form malonyl-CoA. Chloroplast ACCase is a multienzyme complex composed of biotin carboxylase and carboxyltransferase (CT), which is made up of α_2β_2 heterodimer. The CT β polypeptide has the conserved zinc forger-like domain (C-x_2-C-x_<(12-15)>-C-x_2-C) in the N-terminal region. We examined the role of this domain of pea chloroplast CT. The recombinant enzymes without this domain did not have the enzymatic activities. The measurements of metal contents by ruing the inductively coupled argon plasma spectrometer showed that this domain contains the zinc ions. Additionally, the zinc chelator 1,10-o-phenanthroline inhibited the enzymatic activities. These results indicate that the conserved zinc forger-like domain has the ability to bind the zinc ions, and that zinc-binding activity is required for the enzymatic activities.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Kozaki, A.他: "Thiol-Disulfide Exchange between Nuclear-encoded and Chloroplast-encoded Subunits of Pea Acetyl-CoA Carboxylase"Journal of Biological Chemistry. 276. 33919-33925 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Madoka, Y.他: "Chloroplast Transformation with Modified accD Operon Increases Acetyl-CoA Carboxylase and Causes Extension of Leaf Longevity sad Increase is Seed Yield in Tobacco"Plant and Cell Physiology. 43. 1518-1525 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kozaki, A. et al.: "Thiol-Disulfide Exchange between Nuclear-encoded and Chloroplast-encoded Subunits of Pea Acetyl-CoA Carboxylase"Journal of Biological Chemistry. 276-43. 39919-39925 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Madoka, Y. et al.: "Chloroplast transformation with modified accD operon increases acetyl-CoA carboxylase and causes extension of leaf longevity and increase in seed yield in tobacco"Plant and Cell Physiology. 43-12. 1518-1525 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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