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2003 Fiscal Year Final Research Report Summary

GnRH in fish leukocytes

Research Project

Project/Area Number 13460084
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionThe University of Tokyo

Principal Investigator

SUZUKI Yuzuru  The University of Tokyo, Graduate School of Agricultural and Life Science, Professor, 大学院・農学生命科学研究科, 教授 (40107412)

Co-Investigator(Kenkyū-buntansha) SUETAKE Hiroaki  The University of Tokyo, Graduate School of Agricultural and Life Science, Research associate, 大学院・農学生命科学研究科, 助手 (00334326)
AIDA Katsumi  The University of Tokyo, Graduate School of Agricultural and Life Science, Professor, 大学院・農学生命科学研究科, 教授 (50012034)
Project Period (FY) 2001 – 2003
KeywordsGnRH / leukocyte / fish / reproductive endocrine system / immunity / fugu
Research Abstract

Fugu, which has very small genome size, was found to have three types of GnRH, i.e., sbGnRH, sGnRH and cGnRH II, and three GnRH receptors (GnRH-R). We determined their primary structures by cDNA cloning. RT PCR analyses showed that sbGnRH and sGnRH are expressed in various tissues including lymphoid organs and leukocytes, while cGnRH II is expressed only in the kidney, the head kidney and the spleen in addition to the brain, indicatin. Expression of GnRH-R was also recognized in variety of tissues including immune organs and leukocytes. Significance of GnRH in immune system has thus been clearly recognized. Each Affinity of the three types of GnRH against the three GnRH-R expressed on COS cells was tested, and found that all three receptors showed higher affinities against sGnRH and cGnRH II, than against sbGnRH. These results indicate a large variety of the GnRH functions via GnRH-R.
It is essential to classify the lymphocytes for the analysis on the function of GnRH in the immune system. We determined several immune factors of lymphocytes such as, CD3, CD4, CD8, IL-8, IL-12, TCRγ, IgM, and IgD, and now analyzing the effects of immunomodulators, such as PHA, ConA, Poly I : C, LPS and PMA, against those immune factors of lymphocytes, including GnRH.
In addition to these observations, analyses on the relationships between reproductive endocrine system and leukocyte functions. Downregulation of the expression of tyrosine phosphatases by GnRH was also discussed

  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Okubo, Aida 他: "Identification and characterization of two distinct GnRH receptor subtypes in teleost, the medaka, Oryzias latipes."Endocrinology. 142. 4729-4739 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim, Aida, 他: "Effects of olfactory tract section on the immunohistochemical distribution of brain GnRH fibers in the female goldfish, Carassius auratus"Zool.Sci.. 18. 241-248 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okubo, Aida: "Gonadotropin-releasing hormones (GnRHs) on a primitive teleost, the arowana : phylogenetic evidence that three, paralogous lineages of GnRH occurred prior to the emergence of teleosts."Gen.Comp.Endocrinol.. 124. 125-133 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okubo, Aida 他: "Structural characterization of GnRH loci in the medaka genome."Gene. 293. 181-189 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Amano, Aida 他: "Molecular cloning of three cDNAs encoding different GnRHs in the brain of barfin flounder."Gen.Comp.Endoclinol.. 126. 325-333 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okubo, Aida: "Gonadotropin-releasing hormone gene products downregulate the expression of their neighboring genes that encode protein tyrosine phosphatases α and ε."Biochem.Biophys.Res.Communications. 312. 531-536 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saha, Usami, Suzuki: "A double staining flow cytometric assay for the detection of steroid induced apoptotic leucocytes in common carp (Cyprinus carpio)."Dev.Comp.Immunol.. 27. 351-363 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshiura, Suetake, Suzuki 他: "Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunits."Immunogenetics. 55. 296-306 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saha, Suetake, Suzuki: "Characterization and expression of the immunoglobulin light chain in the Fugu : evidence of a solitaire type."Immunogenetics. 56. 47-55 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saha, Usami, Suzuki: "In vitro effects of steroid hormones on the IgM-secreting cells and IgM-secretion in common carp (Cyprinus carpio)."Fish Shellfish Immunol.. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suetake, Suzuki, Aida 他: "Differntial expression of two gonadotropin (GTH) β subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel"Fisheries Sci. 68. 290-298 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okubo, Suetake, Aida: "Three mRNA species for mammarian-type gonadotropin-releasing hormone in the brain of the eel, Anguilla japonica"Mol.Cell.Endocrinol.. 192. 17-25 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saha, Suetake, Suzuki 他: "Relationships between immune and reproductive endocrine systems in fish."Fisheries Sci.. 68 suppleII. 1135-1138 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saha, Usami, Suzuki: "Seasonal changes in the immune activities of common carp (Cyprinus carpio)."Fish Physiol.Biochem.. 26. 379-387 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okubo, Aida 他: "Conserved physical linkage of GnRH-R and RBM8 in the medaka and human genomes."Biochem.Biophys.Res.Communications. 293. 327-331 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木譲, 末武弘章他: "海洋生物の生体防御"月刊海洋. 号外29. 70-75 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okubo, Aida 他: "A novel third gonadotropin-releasing normore receptor in the medaka Oryzias latipes : evolutionary and fuctional implications."Gene. 314. 121-131 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Amano, Aida 他: "Ontogenic origin of Salmon GnRH neurons in the ventral telencephalon and the preoptic area in masu salmon."Gen.Comp.Endoclinol. 127. 256-262 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okubo, K., Nagata, S., Ko, R., Kataoka, H., Yoshiura, Y, Mitani, H., Kondo, M., Naruse, K., Shima, A., Aida, K.: "Identification and characterization of two distinct GnRH receptor subtypes in teleost, the medaka, Oryzias latipes."Endocrinology. 142. 4729-4739 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim, M.H., Kobayashi, M., Oka, Y, Amano, M., Kawashima, S., Aida, K.: "Effects of olfactory tract section on the immunohistochemical distribution of brain GnRH fibers in the female goldfish, Carassius auratus."Zool.Sci.. 18. 241-248 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okubo, K., Aida, K.: "Gonadotropin-releasing hormones (GnRHs) on a primitive teleost, the arowana : phylogenetic evidence that three, paralogous lineages of GnRH occurred prior to the emergence of teleosts"Gen.Comp.Endocrinol.. 124. 125-133 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okubo, K., Mitani, H., Naruse, K., Kondo, M., Shima, A., Tanaka, M., Asakawa, S., Shimizu, N., Yoshiura, Y., Aida, K.: "Structural characterization of GnRH loci in the medaka genome"Gene. 293. 181-189 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amano, M., Takahasi, A., Yamanome, T., Okubo, K., Aida, K., Yamamori, K.: "Molecular cloning of three cDNAs encoding different GnRHs in the brain of barfin flounder"Gen.Com.Endoclinol.. 126. 325-333 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amano, M., Okubo, K., Ikuta, K., Kitamura, S., Okuzawa, K., Yamada, H., Aida, K., Yamamori, K.: "Ontogenic origin of salmon GnRH neurons in the ventral telencephalon and the preoptic area in masu salmon."Gen.Comp.Endoclinol.. 127. 256-262 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suetake, H., Okubo, K., Sato, N., Yoshiura, Y, Suzuki, Y, Aida, I i: "Differential expression of two gonadotropin (GTH) β subunit genes during ovarian maturation induced by repeated injection of salmon GTH in the Japanese eel, Anguilla japonica."Fisheries Sci.. 68. 290-298 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okubo, K., Suetake, H., Aida, K.: "Three mRNA species for mammalian-type gonadotropin-releasing hormone in the brain of the eel, Anguilla japonica"Mol.Cell.Endocrinol.. 192. 17-25 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saha, N.R., Usami, T., Saito, T., Suetake, H., Suzuki, Y.: "Relationships between immune and reproductive endocrine systems in fish."Fisheries Sci.. 68 suppl.II.. 1135-1138 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saha, N.R., Usami, T.and Suzuki, Y.: "Seasonal changes in the immune activities of common carp (Cyprinus carpio)"Fish Physiol.Biochem.. 26. 379-387 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okubo, K., Mitani, H., Naruse, K., Kondo, M., Shima, A., Tanaka, M., Aida, K.: "Conserved physical linkage of GnRH-R and RBM8 in medeka and human genomes"Biochem.Biophys.Res.Communications. 293. 327-331 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, Y, Suetake, H., Tasumi, S., Tsutsui, S., Saha, H.R., Usami, T., Saito, T., Hamuro, K., Yumoto, S., Nakamura, O., Watanabe, T.: "Kaiyoseibutu no seitaibougyo"Gekkan Kaiyo. Suppl.29. 70-75 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okubo, K., Ishii, S., Ishida, J., Mitani, H., Naruse, K., Kondo, M., Shima, A., Tanaka, M., Asakawa, S., Shimizu, N., Aida, K.: "A novel third gonadotropin-releasing hormone receptor in the medaka Oryzias latipes : evolutionary and fuctional implications"Gene. 314. 121-131 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okubo, K, Aida, K.: "Gonadotropin-releasing hormone gene products downregulate the expression of their neighboring genes that encode protein tyrosine phosphatases a and β"Biochem.Biophys.Res.Communications.. 312. 531-536 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saha, N.R., Usami, T., Suzuki, Y.: "A double staining flow cytometric assay for the detection of steroid induced apoptotic leucocytes in common carp (Cyprinus carpio)"Dev.Comp.Immunol.. 27. 351-363 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiura, Y., Kiryu, I., Fujiwara, A., Suetake, H., Suzuki, Y., Nakanishi, T., Ototake, M: "Identification and characterization of Fugu orthologues of mammalian interleukin-12 subunit"Immunogenetics. 55. 296-306 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saha, N.R., Suetake, H., Suzuki, Y.: "Characterization and expression of the immunoglobulin light chain in the Fugu : evidence of a solitaire type"Immunogenetics. 56. 47-55 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saha, N.R., Usami, T, Suzuki, Y.: "In vitro effects of steroid hormones on the IgM-secreting cells and IgM-secretion in common carp (Cyprinus carpio)"Fish Shellfish Immunol.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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