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2002 Fiscal Year Final Research Report Summary

APPLICATION OF GENOME ANALYSIS BY AFLP FINGERPRINT TO CATTLE BREEDING

Research Project

Project/Area Number 13460126
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionKOBE UNIVERSITY

Principal Investigator

TSUJI Soichi  KOBE UNIV. AGRICULTURE PROFESSOR, 農学部, 教授 (10031220)

Co-Investigator(Kenkyū-buntansha) MANNEN Hideyuki  KOBE UNIV. AGRICULTURE ASSOCIATE PROFESSOR, 農学部, 助教授 (20263395)
MUKAI Fumio  KOBE UNIV. AGRICULTURE PROFESSOR, 農学部, 教授 (50093323)
Project Period (FY) 2001 – 2002
KeywordsJAPANESE BLACK / MARKER ASSISTED SELECTION / AFLP / BREEDING VALUE / IMPROVEMENT OF REPRODUCTIVE COWS / BREED IDENTIFICATION / SNPs
Research Abstract

1) BREED IDENTIFICATION; By using DNA markers (SNPs) developed by AFLP fingerprinting, we tried to identify Japanese Black, Holstein and their F1. More than 2000 primer sets were tested between two breeds and found 45 effective breed identifiable AFLP fragments were obtained. By testing 100 animals of each breeds using these 45 AFLP fragments, we could clearly discriminated these animals from each other. This method could prevent or reduce false or miss labeled beef in markets.
2) APPLICATION OF AFLP METHOD TO CATTLE BREEDING; By selecting 48 extreme animals from about 5,000 reproductive cows, 25 were the highest breeding value of beef marbling standard (BMS) and 23 were the lowest breeding value of BMS, a kind of association study was done. By AFLP fingerprinting, DNA from those extreme animals were compared about 500 primer sets, which is equivalent to mapping of more than 2,000 markers on a cattle linkage map and finally get 16 effective AFLP fragments which discriminate two extremes, high and low. Quite higher correlation coefficient, r=0.92 were obtained between breeding value of BMS and general genetic value (GGV) estimated using those 16 fragments. This GGV value was applied to additional 121 animals if the GGV is useful or not. Again higher correlation coefficient between BMS breeding value and GGV, r=0.70, was obtained another animals from the herd with an additional higher correlation coefficient, r=0.63, between rib eye muscle area (REA) breeding value and GGV. In generally, higher correlation between BMS and REA is well known, so that this result is reasonable from the data of statistical genetics. This result offers an epoch-making strategy in animal breeding.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Mannen H.: "Identification of mitochondrial DNA substitutions related to meat quality in Japanese Black cattle"J.Anim.Sci.. 81. 68-73 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimogiri T.: "Linkage mapping of the mitochondrial aconitase (ACO2) gene to chicken chromosome 1"Anim.Genet.. 29. 1 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lee Un Jun: "Localization of the muscular dystrophy AM locus using a chicken linkage map constructed with the Kobe University resource family"Anim.Genet.. 33. 42-48 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mannen H.: "Mitochondrial DNA reveal that domestic goat (Capra hircus) are genetically affected by two subspecies of bezoar (Capra aegagurus)"Biochem.Genet.. 39. 145-154 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 笹崎晋史: "AFLPマーカーを用いた肉用牛5品種の系統分析"日本畜産学会報. 72. J1-J5 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oyama, K.: "Heritability and repeatability estimated for reproductive traits of Japanese Black cows"Asia-Australian J.Anim.Sci.. 15. 1680-1685 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 辻 荘一: "動物遺伝育種学事典"シュプリンガー・ヘアラーク東京. 636 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Mannen, Y. Nagata, & S. Tsuji: "Mitochondrial DNA reveal that domestic goat (Capra hircus) are genetically affected by two subspecies of bezoar (Capra aegagurus)"Biochemical Genetics. 39. 145-154 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] E. J. Lee, K. Yoshizawa, H. Mannen, H. Kikuchi, T. Kikuchi, M. Mizutani, & S. Tsuji: "Localization of the muscular dystrophy AM locus using a chicken linkage map constructed with the Kobe University resource family"Anim. Genet.. 33. 42-48 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Shimogiri, S. Miyagawa, E. J. Lee, H. Mannen, S. Okamoto, Y. Maeda, & S. Tsuji: "Linkage mapping of the mitochondrial aconitase (ACO2) gene to chicken chromosome 1"Anim. Genet.. 33. 312 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Mannen, M. Morimoto, K. Oyama, F. Mukai, and S. Tsuji: "Identification of mitochondrial DNA substitutions related to meat quality in Japanese Black cattle"J. Anim. Sci.. 81. 68-73 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, T., Honda, T., and Mukai, F.: "Inbreeding and effective population size of Japanese Black cattle"J. Anim. Sci.. 79(2). 366-370 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oyama, K., and Mukai, F.: "Optimization of mating design via a simple genetic algorithm with constrained inbreeding and mating frequency in a long-term selection"Anim. Sci. J.. 72(4). 279-284 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, T., Nomura, T., Fukushima, M., and Mukai, F.: "Genetic diversity of a closed population of Japanese Black cattle in Hyogo Prefecture"Anim. Sci. J.. 72(5). 378-385 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, T., Mukai, F., and Yamamoto, A.: "Comparison of selection schemes for achieving desired gains in closed broiler lines"Anim. Sci. J.. 72(5). 386-394 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, T., Nomura, T., Fukushima, M., and Mukai, F.: "Gene dropping analysis of founder contribution in a closed Japanese Black cattle population"Anim. Sci. J.. 73(2). 105-111 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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