2003 Fiscal Year Final Research Report Summary
Restoration of various species of Galliformes using chickens
| Project/Area Number |
13460132
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Shinshu University |
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Principal Investigator |
ONO Tamao Shinshu University, Faculty of Agriculture, Professor, 農学部, 教授 (10177264)
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| Co-Investigator(Kenkyū-buntansha) |
KAGAMI Hiroshi Shinshu University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80308303)
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| Project Period (FY) |
2001 – 2003
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| Keywords | chicken / quail / Blue-breasted quail / embryogenesis / primordial germ cells / chimera / PCR / in situ hybridization |
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| Research Abstract |
In order to rescue the endangered species, and production and preservation of variable breeds in bird, interspecific transfers of primoridal germ cells (PGCs) have been performed. We developed the protocol to obtain a large amount of PGCs from gonads of chicken and quail embryos using PGC specific monoclonal antibodies (anti-SSEA1 and QCR1) and magnetism activated cell sorting (MACS). MACS treatment greatly increased the population ratio of avian PGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. We have worked toward practical use of PCR and in situ hybridization to identify the species specific cell (genome) type among chickens, quail and blue-breasted quail. PGCs of chick embryos were transferred into quail embryos, and the chicken-genome-specific PCR product was observed in the semen of chick-PGC-transferred chimeric quail. We made a progeny test. Females of the putative chimeras were artificially inseminated with chicken semen. We obtained hatchlings but PCR analysis showed that they were hybrid between chicken sperm and donor derived quail ovum. Silky PGCs were introduced to White Leghorn chickens and the prpgeny test is underway.
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Research Products
(36 results)
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[Publications] Obata, K, Koide, M., Nagata, K., Iio, A., Yazawa, S., Ono, T., Yamada, Y., Tuan, R.S., Yokota, M.: "Role of FK506-binding protein 12 in development of the chick embryonic heart."Biochemical and Biophysical Research Communications. 283(3). 621-626 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Harumi, T., Maruyama, K., Kagami, H., Sano, A., Matsubara, Y., Tagami, T., Naito, M.: "Cloning of porcine IGF1 receptor cDNA and detection of sequence polymorphisms using RT-PCR."Animal Genetics. 32(6). 386-389 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Li, Z.D., Liu, C.H., Huang, J.S., Sha, J., Wei, H., Sun, M.J., Zhao, C., Jiang, S.H., Kagami, H.: "Preparation of interspecific chicken (Gallus Domesticus)/duck (Anas Domesticus) chimeras."Acta Zoologica Sinica. 48(4). 543-548 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Obata, K., Koide, M., Nagata, K., Iio, A., Yazawa, S., Ono, T., Sasaki, S., Yamada, Y., Tuan, R.S., Yokota, M.: "Effects of FK506 and rapamycin on formation of the neural tube in chick embryos."Animal Science Journal. 73(3). 229-234 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kagami, H., Yasuda, J., Tagami, T., Naito, M., Matsubara, Y., Harumi, T., Noguchi, T., Yamamoto, Y., Takahashi, T., Matsuyama, J., Komatsu, H., Ono, T.: "Effect of the strain combination in donor and recipient for production efficiency of the W-bearing sperm in mixed-sex germline chimeric chickens."Animal Science Journal. 73(6). 543-456 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Yazawa, S., Obata, K., Iio, A., Koide, M., Yokota, M., Sasaki, S., Kagami, H., Ono, T.: "Heart-selective expression of the chicken FK506-binding protein (FKBP) 12.6 gene during embryonic development."Developmental Dynamics. 226(1). 33-41 (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] Yazawa, S., Obata, K., Iio, A., Koide, M., Yokota, M., Sasaki, S., Kagami, H., Ono, T.: "Gene expression of FK506-binding proteins 12.6 and 12 during chicken development."Comparative Biochemistry and Physiology, Part A. 136(2). 391-399 (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kim, J.N., Kim, M.A., Park, T.S., Kim, D.K., Ono, T., Lim, J.M., Han, J.Y.: "Enriched gonadal migration of donor-derived primordial germ cells by immunomagnetic cell sorting in birds."Molecular Reproduction and Develolment. 68(1). 81-87 (2004)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kim, M.A., Park, T.S., Kim, J.N., Kim, Park, H.J., Lee, Y.M., Ono, T., Lim, J.M., Han, J.Y.: "Production of quail (Coturnix japonica) germline chimeras by transfer of gonadal primordial germ cells into recipient embryos"Theriogenology. 62(印刷中). (2004)
Description
「研究成果報告書概要(和文)」より
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[Publications] Obata, K, Koide, M., Nagata, K., Ito, A., Yazawa, S., Ono, T., Yamada, Y., Tuan, R.S., Yokota, M.: "Role of FK506-binding protein 12 in development of the chick embryonic heart."Biochemical and Biophysical Research Communications. 283(3). 621-626 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Harumi, T., Maruyama, K., Kagami, H., Sano, A., matsubara, Y., Tagami, T., Naito, M.: "Identification of transferred chicken germ cells in quail gonad and semen by amplification of chicken-specific PCR products."Animal Genetics. 32(6). 386-389 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Li, Z.D., Liu, C.H., Huang, J.S., Sha, J., Wei, H., Sun, M.J., Zhao, C., Jiang, S.H., Kagami, H.: "Preparation of interspecific chicken (Gallus Domesticus)/duck (Anas Domesticus) chimeras."Acta Zoologica Sinica. 48(4). 543-548 (2002)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Obata, K., Koide, M., Nagata, K., Iio, A., Yazawa, S., Ono, T., Sasaki, S., Yamada, Y., Tuan, R.S., Yokota, M.: "Effects of FK506 and rapamycin on formation of the neural tube in chick embryos."Animal Science Journal. 73(3). 229-234 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kagami, H., Yasuda, J., Tagami, T., Naito, M., Matsubara, Y., Harumi, T., Noguchi, T., Yamamoto, Y., Takahashi, T., Matsuyama, J., Komatsu, H., Ono, T.: "Effect of the strain combination in donor and recipient for production efficiency of the W-bearing sperm in mixed-sex germline chimeric chickens."Animal Science Journal. 73(6). 453-456 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yazawa, S., Obata, K., Iio, A., Koide, M., Yokota, M., Sasaki, S., Kagami, H., Ono, T.: "Heart-selective expression of the chicken FK506-binding protein (FKBP) 12.6 gene during embryonic development."Developmental Dynamics. 226(1). 33-41 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yazawa, S., Obata, K., Iio, A., Koide, M., Yokota, M., Sasaki, S., kagami, H., Ono, T.: "Gene expression of FK506-binding proteins 12.6 and 12 during chicken development."Comparative Biochemistry and Physiology, Part A.. 136(2). 391-399 (2003)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Kim, J.N., Kim, M.A., Park, T.S., Kim, D.K., Ono, T., Lim, J.M., Han, J.Y.: "Enriched gonadal migration of donor-derived primordial germ cells by immunomagnetic cell sorting in birds."Molecular Reproduction and Develolment.. 68(1). 81-87 (2004)
Description
「研究成果報告書概要(欧文)」より
-
-
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[Publications] Kim, M.A., Park, T.S., Kim, J.N., Kim, Park, H.J., Lee, Y.M., Ono, T., Lim, J.M., Han, J.Y.: "Production of quail (Conturnix japonica) germline chimeras by transfer of gonadal primordial germ cells into recipient embryos."Theriogenology.. 62(in press). (2004)
Description
「研究成果報告書概要(欧文)」より
