2003 Fiscal Year Annual Research Report

哺乳類および鳥類の生体内時系列機構と生体時計機構の解析

Research Project

Project/Area Number	13460134
Research Institution	University of Miyazaki
Principal Investigator	村上昇宮崎大学, 農学部, 助教授 (80150192)
Co-Investigator(Kenkyū-buntansha)	那須哲夫宮崎大学, 農学部, 助教授 (40108725) 塩田邦郎東京大学, 大学院・農学生命科学研究科, 教授 (80196352)
Keywords	ニューロメジンU / 生体リズム / 視交叉上核 / 位相変位 / サーカディアンリズム / 反射
Research Abstract	新規摂食抑制ホルモンであるニューロメジンU(NMU)のノックアウトマウスは行動が緩慢であり、行動リズムが消失していることが判明した。そこで、種々の反射機構を検討した結果、熱反射や痛み反射がNMUノックアウトマウスでは劣っていることが判明した。また、NMUを脳室内に投与するとこれらの反射機構は亢進した。NMUの免疫陽性細胞が脊髄に分布し、またNMUの投与により脊髄にcFosが発現した。NMUノックアウトマウスは光への同調も遅く、環境温度への適応も低下していた。以上のことから、NMUは種々の反射機構を調節するホルモンであると思われた。一方、NMUのレセプターmRNAが時計部位の視交叉上核に存在し、24時間のリズムを示していること、また、NMUの脳室内投与により、視交叉上核にもFosが発現すること、さらにNMUの免疫染色で、視交叉上核の外背側部で染まることから、NMUは視交叉上核内でオートクライン的作用により、時計を調節している可能性が示唆された。また、NMUの類似体として新たに同定したNMSは、体内時計の局在部位である視交叉上核(SCN)に限局して発現しており、脳室内投与によって、投与時刻依存的に、行動リズムの位相変位を起こした。また、位相前進が観察されたサーカディアン時間の6時(CT6)において、NMS投与後SCNでのc-Fos蛋白質の発現および多ニューロン発火活動頻度の上昇が観察された。以上の結果、NMSはSCNの時計機構そのものの構成物質ではなく、時計を調節する物質であることが示唆された。

Research Products

(20 results)

All Other

All Publications (20 results)

[Publications] N.Murakami: "Effect of melatonin on the circad n rhythm, locomotor activity and body temperature in intact house sparrow, Japanese quail and owl"Brain Res. 889(1-2). 220-224 (2001)
[Publications] M.Nakazato: "A role for ghrelin in the central regulation of feeding"Nature. 409. 194-198 (2001)
[Publications] G.Nishimura: "Auto-immunization against intestinal bacterial endotoxin prevents alcoholic fatty liver"J.Vet.Med.Sci.. 63. 275-280 (2001)
[Publications] Y.Date: "Ghrelin Acts in the Central Nervous System to Stimulate Gastric Acid Secretion"Biochem Biophys Res Commun. 280(3). 904-907 (2001)
[Publications] K.Toshinai: "Upregulation of ghrelin expressin in the stomach upon fasting, insulin-induced hypoglycemia and leptin administration"Biochem Biophys Res Commun. 281. 1220-1225 (2001)
[Publications] K Nakahara: "Intracellular Ca^<2+> signaling pathway is in volved in light-induced phase advance, cell. but may not be in phase delay, of o'rcadian melatonin rhythm in chick pineal"Journal of Pineal Research. 30. 234-242 (2001)
[Publications] T.Hayashida: "Ghrelin in domestic animals : distribution in stomach and its possible role Domestic Animal"Endocrinology. 21(1). 17-24 (2001)
[Publications] R.Hanada: "A role for neuromedin U in stress response"Biochem Biophys Res Commun. 289. 225-228 (2001)
[Publications] K.Nakahara: "Pituitary adenylate cyclase-activating polypeptide (PACAP) is involved in melatonin release via the speciffic receptor PACAP-rl, but not in the circadian oscillar, in chick pineal cells"Brain Research. 939. 19-25 (2002)
[Publications] T Hayashida: "Ghrelin in neonatal rats : distribution in stomach and its possible role"J.Endocrinology. 173. 239-245 (2002)
[Publications] N Murakami: "Role for central ghrelin in food intake and secretion profile of stomach ghrelin in rats"J.Endocrinology. 174. 283-288 (2002)
[Publications] Y.Date: "The role of gastric vagal afferent in feeding and growth hormone secretion effects of ghrelin"Gastroenterology. 123(4). 1120-1128 (2002)
[Publications] J C Rivero: "Androgen-and estrogen dependent sex difference in host resistance to Strongyloides venezuelensis infection in Wistar rats"J.Vet.Med.Sci.. 64. 457-461 (2002)
[Publications] J C Rivero: "Age-and sex-related changes in susceptibity of Wistar rats to strongyloides venezuelensis infection"J.Vet.Med.Sci.. 64. 519-521 (2002)
[Publications] Toshinai K: "Ghrelin-induce food intake is mediated via the orexin pathway"Endocrinology. 144(4). 1506-1512 (2003)
[Publications] Shimada M: "Somatostatin suppresses ghrelin secretion from the rat stomach"Biochem Biophys Res Commun. 302. 520-525 (2003)
[Publications] Mondal M S: "Nauromedin U Acts in the Central Nervous System to Inhibit Gastric Acid Secretion via Corticotropin-Releasing Hormone system"Am J Physiol Gastrointest Liver Physiol.Jun. 284(4). G963-G969 (2003)
[Publications] K Nakahara: "Effect of Chronic Treatments with Ghrelin on Milk Secretion in Lactating Rats"Biochem Biophys Res Commun. 303(3). 751-755 (2003)
[Publications] K Nakahara: "Effects of microinjection of melatonin into various brain regions of Japanese quail on the locomotor activity and body temperature"Neuroscience Letter. 345(2). 117-120 (2003)
[Publications] T Hanada: "Central actions of neuromedin U via corticotropin-releasing hormone"Biochem Biophys Res Commun.2003 Nov 28. 311(4). 954-958 (2003)

2003 Fiscal Year Annual Research Report

哺乳類および鳥類の生体内時系列機構と生体時計機構の解析

Principal Investigator

村上 昇 宮崎大学, 農学部, 助教授 (80150192)

Research Products

[Publications] N.Murakami: "Effect of melatonin on the circad n rhythm, locomotor activity and body temperature in intact house sparrow, Japanese quail and owl"Brain Res. 889(1-2). 220-224 (2001)

[Publications] M.Nakazato: "A role for ghrelin in the central regulation of feeding"Nature. 409. 194-198 (2001)

[Publications] G.Nishimura: "Auto-immunization against intestinal bacterial endotoxin prevents alcoholic fatty liver"J.Vet.Med.Sci.. 63. 275-280 (2001)

[Publications] Y.Date: "Ghrelin Acts in the Central Nervous System to Stimulate Gastric Acid Secretion"Biochem Biophys Res Commun. 280(3). 904-907 (2001)

[Publications] K.Toshinai: "Upregulation of ghrelin expressin in the stomach upon fasting, insulin-induced hypoglycemia and leptin administration"Biochem Biophys Res Commun. 281. 1220-1225 (2001)

[Publications] K Nakahara: "Intracellular Ca^<2+> signaling pathway is in volved in light-induced phase advance, cell. but may not be in phase delay, of o'rcadian melatonin rhythm in chick pineal"Journal of Pineal Research. 30. 234-242 (2001)

[Publications] T.Hayashida: "Ghrelin in domestic animals : distribution in stomach and its possible role Domestic Animal"Endocrinology. 21(1). 17-24 (2001)

[Publications] R.Hanada: "A role for neuromedin U in stress response"Biochem Biophys Res Commun. 289. 225-228 (2001)

[Publications] K.Nakahara: "Pituitary adenylate cyclase-activating polypeptide (PACAP) is involved in melatonin release via the speciffic receptor PACAP-rl, but not in the circadian oscillar, in chick pineal cells"Brain Research. 939. 19-25 (2002)

[Publications] T Hayashida: "Ghrelin in neonatal rats : distribution in stomach and its possible role"J.Endocrinology. 173. 239-245 (2002)

[Publications] N Murakami: "Role for central ghrelin in food intake and secretion profile of stomach ghrelin in rats"J.Endocrinology. 174. 283-288 (2002)

[Publications] Y.Date: "The role of gastric vagal afferent in feeding and growth hormone secretion effects of ghrelin"Gastroenterology. 123(4). 1120-1128 (2002)

[Publications] J C Rivero: "Androgen-and estrogen dependent sex difference in host resistance to Strongyloides venezuelensis infection in Wistar rats"J.Vet.Med.Sci.. 64. 457-461 (2002)

[Publications] J C Rivero: "Age-and sex-related changes in susceptibity of Wistar rats to strongyloides venezuelensis infection"J.Vet.Med.Sci.. 64. 519-521 (2002)

[Publications] Toshinai K: "Ghrelin-induce food intake is mediated via the orexin pathway"Endocrinology. 144(4). 1506-1512 (2003)

[Publications] Shimada M: "Somatostatin suppresses ghrelin secretion from the rat stomach"Biochem Biophys Res Commun. 302. 520-525 (2003)

[Publications] Mondal M S: "Nauromedin U Acts in the Central Nervous System to Inhibit Gastric Acid Secretion via Corticotropin-Releasing Hormone system"Am J Physiol Gastrointest Liver Physiol.Jun. 284(4). G963-G969 (2003)

[Publications] K Nakahara: "Effect of Chronic Treatments with Ghrelin on Milk Secretion in Lactating Rats"Biochem Biophys Res Commun. 303(3). 751-755 (2003)

[Publications] K Nakahara: "Effects of microinjection of melatonin into various brain regions of Japanese quail on the locomotor activity and body temperature"Neuroscience Letter. 345(2). 117-120 (2003)

[Publications] T Hanada: "Central actions of neuromedin U via corticotropin-releasing hormone"Biochem Biophys Res Commun.2003 Nov 28. 311(4). 954-958 (2003)

村上昇宮崎大学, 農学部, 助教授 (80150192)