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2004 Fiscal Year Final Research Report Summary

Search of developmental and inhibitory factors in pathogenesis of enzootic bovine leukosis

Research Project

Project/Area Number 13460138
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionIwate University

Principal Investigator

OKADA Kosuke  Iwate University, Faculty of Agriculture, Professor, 農学部, 教授 (50002077)

Co-Investigator(Kenkyū-buntansha) AIDA Yoko  RIKEN, Retrovirus Research Unit, Unit leader, 分子ウイルス学研究ユニット, ユニットリーダー (82401999)
ONUMA Misao  Hokkaido University, Graduate School of Veterinary Medicine, Professor, 大学院・獣医学研究科, 教授 (70109510)
GORYO Masanobu  Iwate University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80153774)
HIRATA Toh-ichi  Iwate University, Faculty of Agriculture, Research Associate, 農学部, 助手 (20241490)
Project Period (FY) 2001 – 2004
Keywordsbovine leukemia virus / CD5 B cells / enzootic bovine leukosis / lymphoma / MHC class II / persistent lymphocytosis / TNF-RI / TNF-RII
Research Abstract

Enzootic bovine leukosis (EBL) is caused by bovine leukemia virus (BLV). Because EBL occurs frequently and the BLV-infection rate is very high, the economic damage is serious in the Tohoku area. On the other hand, it has been demonstrated that BLV and human T-cell leukemia virus, which induces human adult T-cell leukemia, constitute a unique subgroup within the retrovirus family. This subgroup is characterized by distinct genetic content, genomic organization, and strategy for gene expression including the pX gene, so more expectation is placed on EBL as a good model for understanding human malignant diseases. However, there are many unclear points, such as the mechanism of oncogenesis due to BLV infection and pathogenesis in lymphoid organs. A few BLV-infected cattle develop leukemia after a long latency period. Many of the infected animals become serologically positive for the anti-BLV antibody, but they remain in a healthy, subclinical carrier state. Of the infected animals, 30 to 35% develop persistent lymphocytosis (PL). In these animals, BLV provirus dramatically increases in BoCD5-positive B-1a cells, and expression of the viral gene increases. Less than 1% of infected animals develop leukemia and lymphoma resulting in death. During the present study, which spans a 4-year period, it became clear that 1)the difference in the activity types of viral tax gene does not effect the pathogenicity in sheep, 2)the major histocompatibility antigen (MHC) haplotypes are closely related to the resistance and sensitivity to BLV-induced lymphoma, 3)humeral and cellular immune responses are strongly induced against viruses in sheep with BLV-resistant MHC allele, 4)the inhibition of lymphocytic apotosis occurs in BLV-infected sheep, so proliferation of lymphocytes will be promoted, and 5)expression of tumor necrosis factor receptors type 2 increase in cattle with PL or EBL. Thus, a number of the factors associated with the development and inhibition of EBL became clear.

  • Research Products

    (26 results)

All 2005 2004 2003 2002 2001

All Journal Article (25 results) Book (1 results)

  • [Journal Article] Imrnunohistochemical analysis of expression patterns of tumor necrosis factor receptors on lymphoma cells in enzootic bovine leukosis.2005

    • Author(s)
      Ikeda, M., Konnai, S., Onuma, M., Ishiguro, N., Goryo, M., Okada, K.
    • Journal Title

      J.Vet.Med.Sci. 67

      Pages: 425-432

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ex vivo survival of peripheral blood mononuclear cells in sheep induced by bovine leukemia virus (BLV) mainly occurs in CD5 B cells that express BLV.2004

    • Author(s)
      Takahashi, M., Tajima, S., Takeshima, S., Konnai, S., Yin, S., Okada, K., Davis, W.C., Aida, Y.
    • Journal Title

      Microb.Infec. 6

      Pages: 584-595

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Delayed-type hypersensitivity in sheep induced by synthetic peptids of bovine leukemia virus encapsulated in mannan-coated liposme.2003

    • Author(s)
      Okada K.et al.
    • Journal Title

      J.Vet.Med.Sci. 65

      Pages: 515-518

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Relation between phenotype of tumor cells and clinicopathology in bovine leukosis.2003

    • Author(s)
      Yin S.et al.
    • Journal Title

      J.Vet.Med.Sci. 85

      Pages: 599-606

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A mutant form of the Tax protein of bovine leukemia virus (BLV), with enhanced transactivation activity, increases expression and popagation2003

    • Author(s)
      Tajima S.et al.
    • Journal Title

      J.Virol. 77

      Pages: 1894-1903

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The influence of ovine MHC class II DRB1 alleles on immuneresponse in bovine leukemia virus infection.2003

    • Author(s)
      Konnai S.et al.
    • Journal Title

      Microbiol.Immunol. 47

      Pages: 223-232

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 御明神牧場におけるウシ白血病ウイルス接種実験概要2003

    • Author(s)
      岡田 幸助 他
    • Journal Title

      岩手大学農学部附属寒冷フィールドサイエンス教育研究センター年報 1

      Pages: 69-78

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunohistochemical analysis of expression patterns of tenor necrosis factor receptors on lymphoma cells in enzootic bovine leukosis.2003

    • Author(s)
      Ikeda M.et al.
    • Journal Title

      J.Vet.Med.Sci. 47

      Pages: 425-432

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Delayd-type hypersensitivity in sheep induced by synthetic peptids of bovine leukemia virus encapsulated in mannan-coated liposome.2003

    • Author(s)
      Okada, K., Sonoda, K., Koyama, M., Yin, S., Ikeda, M., Goryo, M., Chen, SL., Kabeya, H., Ohashi, K, Onuma, M.
    • Journal Title

      J.Vet.Med.Sci. 65

      Pages: 515-518

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Relation between phenotype of tumor cells and clinicopathology in bovine leukosis.2003

    • Author(s)
      Yin, S., Makara, M., Pan, Y., Ishiguro, H., Ikeda, M., Numakunai, S., Goryo, M, Okada, K.
    • Journal Title

      J.Vet.Med.Sci. 65

      Pages: 599-606

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A mutant form of the Tax protein of bovine leukemia virus (BLV), with enhanced transactivation activity, increases expression and propagation of BLV in vitro but not in vivo.2003

    • Author(s)
      Tajima, S., Takahashi, M., Takeshima, S., Konnai, S., Yin, S.A., Watarai, S.T., Tanaka, Y., Onuma, M., Okada, K, Aida, Y.
    • Journal Title

      J.virol. 77

      Pages: 1894-1903

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The influence of ovine MHC class II DRB1 alleies on immune response in bovine leukemia virus infection.2003

    • Author(s)
      Konnai, S., Takeshima, S., Tajima, S., Yin, S., Okada, K., Onuma, M., Aida, Y.
    • Journal Title

      Microbiol.Immunol. 47

      Pages: 223-232

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Sequence and diversity of 17 new Gvar-DRB1 alleies from three breeds of sheep.2003

    • Author(s)
      Konnai, S., Nagaoka, Y., Takeshima, S., Onuma, M., Aida, Y.
    • Journal Title

      Eur.J.Immunogenet. 30

      Pages: 275-282

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] DNA typing for ovine MHC DRB1 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).2003

    • Author(s)
      Konnai, S., Nagaoka, Y., Takesima, S., Onuma, M., Aida, Y.
    • Journal Title

      J.Dairy Sci. 86

      Pages: 3362-3365

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The rapid quantitative analysis of bovine cytokine genes by real-time RT-PCR.2003

    • Author(s)
      Konnai, S., Usui, T., Ohashi, K., Onuma, M.
    • Journal Title

      Vet.Microbiol. 94

      Pages: 283-294

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Latency of viral expression in vivo is not related to CpG methylation in the U3 and part of the R region of the long terminal repeat of bovine leukemia virus.2003

    • Author(s)
      Tajima, S., Tsukamoto, M., Aida, Y.
    • Journal Title

      J.virol. 77

      Pages: 4423-4430

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The diversity of bovine MHC class II DRB3 genes in Japanese Black, Japanese Shorthorn, Jersey and Holstein cattle.2003

    • Author(s)
      Takeshima, S., Saito, N., Morita, M., Inoko, H., Aida, Y.
    • Journal Title

      Gene. 316

      Pages: 111-118

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Protective effects of vaccination with bovine leukemia virus (BLV) Tax DNA against BLV infection in sheep.2003

    • Author(s)
      Usui, T., Konnai, S., Tajima, S., Watarai, S., Aida, Y., Ohashi, K., Onuma, M.
    • Journal Title

      J.Vet.Med.Sci. 65

      Pages: 1201-1205

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Seroprevalence of bovine immunodeficiency virus and bovine leukemia virus in dairy and beef cattle in Hokkaido.2003

    • Author(s)
      Usui, T., Meas, S., Konnai, S., Ohashi, K., Onuma, M.
    • Journal Title

      J.Vet.Med.Sci. 65

      Pages: 287-289

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mutant Tax proteins from bovine leukemia virus with ability to activate the expression of c-fos.2002

    • Author(s)
      Tajima, S, Aida, Y.
    • Journal Title

      J.Virology. 76

      Pages: 2557-2562

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of DRB3 alleies in the MHC of Japanese Shorthorn cattle by polymerase chain reaction-sequence-based typing.2002

    • Author(s)
      Takeshima, S., Nakai, Y., Morita, M, Aida, Y.
    • Journal Title

      J.Dairy Sci. 85

      Pages: 1630-1632

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Tumor necrosis factor α and its receptors in experimentally BLV-infected sheep.2001

    • Author(s)
      Kabeya, H., Fukuda, A., Ohashi, K., Sugimoto, C, Onuma, M.
    • Journal Title

      Vet.Immunol.Immunopathol. 81

      Pages: 129-139

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Host immune responses in the course of BLV infection.2001

    • Author(s)
      Kabeya, H., Ohashi, K., Sugimoto, C, Onuma, M.
    • Journal Title

      J.Vet.Med.Sci. 63

      Pages: 895-901

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Growth inhibition of cancer cells by co-transfection of diphtheria toxin A-chain gene plasmid with bovine leukemia virus-Tax expression vector.2001

    • Author(s)
      Tana, Watarai, S., Aida, Y., Tajima, S., Kaikidani, H., Onuma, M, Kodama, M.
    • Journal Title

      Microbiol.Immunol. 45

      Pages: 447-455

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of new bovine BOLA-DRB3 alleies by sequence-based typing.2001

    • Author(s)
      Takeshima, S., Ikegami, M., Morita, M., Nakai, Y, Aida, Y.
    • Journal Title

      Immunogenetics. 53

      Pages: 74-81

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 動物病理学総論 第2版2001

    • Author(s)
      岡田幸助
    • Total Pages
      268
    • Publisher
      文永堂
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2006-07-11  

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