2002 Fiscal Year Final Research Report Summary
Isolation and characterization of stem cell-derived neural stem/progenitor cell supporting factor, SDNSF.
| Project/Area Number |
13470036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
TASHIRO Kei Kyoto University, Center for Molecular Biology and Genetics, Assistant Professor, 遺伝子実験施設, 助教授 (10263097)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Jun Kyoto University, Graduate School of Medicine, Research Associate, 医学研究科, 助手 (10270779)
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| Project Period (FY) |
2001 – 2002
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| Keywords | stem cell / inner ear / secreted molecule / signal-sequence trap / otocyst / soluble factor / rat / development |
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| Research Abstract |
Recent evidences suggests that adult neural stem/progenitor cells (ANSCs) secrete autocrine/paracrine factors and that these intrinsic factors are involved in the maintenance of adult neurogenesis. We identified a novel secretory molecule, stem cell-derived neural stem/progenitor cell supporting factor (SDNSF), from adult hippocampal neural stem/progenitor cell s by using the signal sequence trap method. The expression of SDNSF in adult central nervous system was localized to hippocampus including dentate gyrus, where the neurogenesis persists throughout life. In induced neurogenesis status seen in ischemically treated hippocampus, the expression of SDNSF was up-regulated. As functional aspects, SDNSF protein provided a dose-dependent survival effect for ANSC following basic fibroblast growth factor 2 (FGF-2) withdrawal. ANSCs treated by SDNSF also retain self-renewal potential and multipotency in the absence of FGF-2. However, SDNSF did not have mitogenic activity, nor was it a cofactor that promoted the mitogenic effects of FGF-2. These data suggested an important role of SDNSF as an autocrine/paracrine factor in maintaining stem cell potential and lifelong neurogenesis in adult central nervous system.
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