2002 Fiscal Year Final Research Report Summary
Etiological agents responsible for systemic lupus erythrmatosus
| Project/Area Number |
13470106
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Tohoku University |
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Principal Investigator |
SASAKI Takeshi Tohoku Univ., Graduate Sch. Med., Professor, 大学院・医学系研究科, 教授 (50110656)
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| Co-Investigator(Kenkyū-buntansha) |
MUNAKATA Yasuhiko Tohoku Univ., Hospital, Research Associate, 医学部附属病院, 助手 (20271950)
HARIGAE Hideo Tohoku Univ., Hospital, Instructor, 医学部附属病院, 助手 (50302146)
KAMEOKO Jynichi Tohoku Univ., Hospital, Research Associate, 医学部附属病院, 助手 (30261621)
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| Project Period (FY) |
2001 – 2002
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| Keywords | systemic lupus erythematosus / anti-DNA antibody / lupus nephritis |
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| Research Abstract |
1. First, we confirmed that human monoclonal anti-DNA antibody O-81 would be nephritogenic antibodies responsible for tissue injury in systemic lupus erythematosus(SLE). We established SLE model in SCID mice, where active SLE lymphocytes were injected.
The coexistence of anti-81 ribozyme prevented the formation of immune deposits and elevation of anti-DNA activity in vivo.
2. O-81 scFV was obtained by using E coil-Pin Point TM system for the expression of protein from O-81 VH gene. O-81 scFV bound to DNA and anti-idiotypic antibodies to O-81. We also obtained cDNA library originated from SLE lymphocytes. The peptides expressing on the clones derived from SLE cDNA library, were examined for the binding ability to O-8 l scFV. As a result, 16 clones were obtained and the recombinant protein was obtained from some clones.
3. The recombinant protein reacted with O-81 indicating. This indicates that the determined protein may be specific to SLE.
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