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2002 Fiscal Year Final Research Report Summary

Development of strategic therapy for severe hepatic failure using regenerative medicine-Isolation of hepatic stem cell expressing musashi-1 gene and cell therapy

Research Project

Project/Area Number 13470112
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionYamagata University

Principal Investigator

KAWATA Sumio  Yamagata Unv. Medical Sch., Professor, 医学部, 教授 (90183285)

Co-Investigator(Kenkyū-buntansha) SAITO Koji  Yamagata Unv. Medical Sch., Assistant Prof., 医学部, 講師 (90250919)
SAITO Takafumi  Yamagata Unv. Medical Sch., Assistent Prof., 医学部, 講師 (80250918)
TOGASHI Hitoshi  Yamagata Unv. Medical Sch., Associate Prof., 医学部, 助教授 (60192209)
WATANABE Hisayoshi  Yamagata Unv. Medical Sch., Assistant, 医学部, 助手 (00332536)
Project Period (FY) 2001 – 2002
KeywordsHepatic Stem cell / Musashi-1 gene / oval cell / hepatocytes / Hepatoma cell lines
Research Abstract

We are trying to develop an innovative therapy for sever hepatic failure to transplant hepatic stem cell. In this therapy, it is an important step to isolate hepatic stem cell expressing musashi-1 gene.
In 2001 we established a cell line of oval cells, bipotent stem cells, from rat livers. This cell line expressed musashi-1 gene with AFP and CK-18 ones. With differenciation of the cell line by spheroid formation, the musashi-1 gene expression was suppressed. Moreover, three of seven human hepatoma cell lines expressed musashi-1 gene. This implied that musashi-1 gene might participate in differentiation of hepatic stem cells and hepatic carcinogenesis.
In 2002, we found that the musashi-1 gene regulated notch signalin via suppression of Numb gene expression. Also, role of musashi-1 gene in liver development is under investigation using knockout mice of the gene.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Shu H-J, Saito T, Kawata S, et al.: "Expression of the Musashi1 gene encoding the RNA-binding protein in human hepatoma cell lines"Biochem.Biophys.Res.Commun.. 293. 150-154 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terui Y, Saito T, Kawata S, et al.: "Effect of angiotensin receptor antagonist on liver fibrosis of chronic hepatitis C"Hepatology. 36. 1022 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito T, Shinzawa H, Kawata S, et al.: "Lamivudine and rapid regeneration of the atrophic liver in decompensated cirrhosis due to Hepatitis B"Am J Gastroenterol. 97. 493-494 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saeki A, Tamura S, Kawata S, et al.: "Frequent impairment of the spindle assembly checkpoint in hepatocellular carcinoma"Cancer. 94. 2047-2054 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakon M, Nagano H, Kawata S et al.: "Combined intraarterial 5-FU and subcutaneous IFN-αtherapy for advanced hepatocellular carcinoma with tumor thrombi in the major portal branches"Cancer. 94. 435-442 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito T, Shinzawa H, Kawata S, et al.: "Home lactulose enema: prevention of hepatic encephalopathy at home"J.Gastroenterol. 97. 493-495 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shu H-J, Saito T, Kawata S, et al.: "Expression of the musashi-1 gene encoding the RNA-binding protein in human hepatoma cell lines"Biochem. Biophys. Res Commun.. 293. 150-154 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terui Y, Saito T, Kawata S, et al.: "Effect of angiotensin receptor antagonist on liver fibrosis of chronic hepatitis C"Hepatology. 36. 1022-1023 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito T, Shinzawa H, Kawata S, et al.: "Lamivudine and rapid regeneration of the atrophic liver in decompensated cirrhosis due to HBV"Am. J. Gastroenterol. 97. 493-494 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saeki A, Tamura S, Kawata S, et al.: "Frequency impairment of the spindle assembly checked point in hepatocellular carcinoma"Cancer. 94. 2047-2054 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakon M, Nagano H, Kawata S, et al.: "Combined intraarterial 5-FU and subcutaneous IFN-a therapy for advanced hepatocellular carcinoma"Cancer. 94. 435-442 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito T, Shinzawa H, Kawata S, et al.: "Home lactulose enema:prevention of hepatic encephalopathy at home"J. Gastroenterol. 37. 68-69 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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