Apoptosis and anti-apoptosis in pancreatic diseases

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 13470123
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: OTSUKI Makoto University of Occupational and Environmental Health, Japan, Third Department of Internal Medicine, Professor and chair, 医学部, 教授 (00030916)
• Co-Investigator(Kenkyū-buntansha): TAGUCHI Masashi University of Occupational and Environmental Health, Japan, Third Department of Internal Medicine, Research Associate, 医学部, 助手 (80369058) ; NAKAMURA Hayato University of Occupational and Environmental Health, Japan, Third Department of Internal Medicine, Associate Professor, 医学部, 助教授 (90207902) ; TASHIRO Mitsuo University of Occupational and Environmental Health, Japan, Third Department of Internal Medicine, Research Associate, 医学部, 助手 (20341498)
• Project Period (FY): 2001 – 2003
• Keywords: survivin / acute pancreatitis / apoptosis / antiapoptotic effect / regeneration / proliferation

Research Abstract

Survivin is one of the inhibitors of the apoptosis family and has dual effects : antiapoptotic effect and regulation of the cell cycle. To show involvement of survivin in acute pancreatitis, acute necrohemorrhagic pancreatitis was induced in male Wistar rats by intraductal infusion of 4% sodium taurocholate. By Northern blotting, the survivin mRNA level was significantly increased at 36 hours and peaked at 48 hours after induction of acute pancreatitis. Survivin protein was found in cytoplasm of ductal cells by immunohistochemical analysis at 48-72 hours. It was also observed in nuclei of both acinar and ductal cells as well as infiltrating cells. Apoptotic cells were observed in pancreatic acinar cells. Survivin protein partially colocalized with 5-bromo-2'-deoxyuridine in some nuclei of ductal cells. In conclusion, we showed involvement of survivin in acute pancreatitis in rats. Survivin may have some roles in the regulation of pancreatic regeneration and proliferation as well as an antiapoptotic effect after acute pancreatitis.

Research Products

• [Publications] Tashiro M et al.: "Expression of survivin after acute necrohemorrhagic pancreatitis in rats."Pancreas. 26. 160-165 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakamura H et al.: "Increased expression of 19-KD interacting protein-3-like protein and the relationship to apoptosis in the lung of rats with severe acute pancreatitis."Critical Care Medicine. 31. 2527-2534 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tashiro M, Nakamura H, Taguchi M, Yoshikawa H, Otsuki M.: "Expression of survivin after acute necrohemorrhagic pancreatitis in rats."Pancreas. 26(2). 160-165 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakamura H, Honda H, Tashiro M, Taguchi M, Yoshikawa H, Otsuki M.: "Increased expression of 19-KD interacting protein-3-like protein and the relationship to apoptosis in the lung of rats with severe acute pancreatitis."Critical Care Medicine. 31(10). 2527-2534 (2003)
  • Description: 「研究成果報告書概要(欧文)」より