2003 Fiscal Year Final Research Report Summary
Development of intracellular targeting liposomes for boron neutron-capture therapy
| Project/Area Number |
13470190
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Teikyo University |
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Principal Investigator |
MARUYAMA Kazuo Teikyo University, School of Pharmaceutical Science, Professor, 薬学部, 教授 (30130040)
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| Co-Investigator(Kenkyū-buntansha) |
YANAGIE Hironobu University of Tokyo, RCAST, Associate Professor, 先端科学技術センター, 助教授 (30212278)
KASAOKA Satoshi Teikyo University, School of Pharmaceutical Science, Research Instructor, 薬学部, 助手 (90338690)
TAKIZAWA Tomoko Teikyo University, School of Pharmaceutical Science, Research Instructor, 薬学部, 助手 (90260934)
ONO Koji Kyoto University, Institute For Atomic Reactor, Professor, 原子炉実験場, 教授 (90122407)
SHINOHARA Atsuko Juntendo University School of Medicine, Assistant Professor, 医学部, 講師 (90157850)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Neutron-Capture Therapy / DDS / Liposome / Transferrin |
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| Research Abstract |
The successful treatment of cancer by BNCT requires the selective concentration of ^<10>B within malignant tumor cells. Intracellular targeting ability and cytotoxic effects of ^<10>B entrapped TF-PEG-liposomes, in which TF is covalently linked to the distal terminal of PEG chains on the external surface of PEG-liposomes, were examined in Colon 26 tumor-bearing mice. TF-PEG-liposomes readily bound to tumor cells in vitro, and were internalized by receptor-mediated endocytosis. ^<10>B-PEG-liposomes and ^<10>B-TF-liposomes showed prolonged residence time in the circulation and low RES uptake in tumor-bearing mice, resulting in enhanced extravasation of the liposomes into the solid tumor tissue and reached high level of ^<10>B content in tumor. After thermal neutron irradiation of mice injected with ^<10>B-PEG-liposomes or ^<10>B-TF-liposomes, tumor growth was suppressed relative to controls. These results suggest that intravenous injection of ^<10>B TF-PEG-liposome can increase the intracellular retention of ^<10>B atoms, which were introduced by receptor mediated endocytosis after binding, causing tumor growth suppression in vivo upon thermal neutron irradiation.
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